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Application of dermatopontin in preparing kit for detecting cardiac failure and detection kit

A heart failure and kit technology, which is applied to the application field of skin pontin in the preparation of heart failure detection kits, can solve the problems of low detection efficiency and difficult to obtain heart failure, and achieves low cost, high serum dilution ratio and high efficiency. high effect

Inactive Publication Date: 2010-07-21
FUWAI HOSPITAL OF CARDIOVASCULAR DESEASE CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using traditional molecular biology methods can only analyze the expression of one or several genes at a time, so the detection efficiency is low, that is to say, it is difficult to obtain all the genes with expression changes related to heart failure by using traditional methods.

Method used

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  • Application of dermatopontin in preparing kit for detecting cardiac failure and detection kit
  • Application of dermatopontin in preparing kit for detecting cardiac failure and detection kit
  • Application of dermatopontin in preparing kit for detecting cardiac failure and detection kit

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Experimental program
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Effect test

Embodiment 1D

[0038] Embodiment 1DPT and heart failure relation experiment

[0039] The etiology of arrhythmogenic right ventricular heart disease (ARVC) is still unclear, and the main feature is that the right ventricular myocardium is replaced by fibrofatty tissue, causing repeated ventricular arrhythmias. Our 5 heart failure patients with ARVC mainly showed left ventricular dysfunction, and the pathology confirmed that the left ventricular free wall was replaced by fibrofatty tissue. Each specimen was obtained from the left ventricular free wall of a failing heart after heart transplantation and a non-failing donor heart. Some specimens were immediately frozen and stored in liquid nitrogen for gene chip and real-time PCR analysis, and the rest were fixed in 10% formalin for pathological examination and immunohistochemical analysis.

[0040] 1. Specific steps:

[0041] 1) Gene chip: use the gene chip to detect the genes differentially expressed in the myocardial tissues of ARVC patients...

Embodiment 2

[0051] Embodiment 2 uses kit of the present invention to detect the expression of serum DPT in end-stage heart failure group and normal group

[0052] Ninety-six cases of end-stage heart failure and 100 healthy controls were used to detect the expression of serum DPT in end-stage heart failure group and normal group, and the sensitivity and specificity of DPT in detecting heart failure were analyzed by ROC curve of SPSS17.0 software.

[0053] 1. Specific steps: DPT was assessed by enzyme-linked immunosorbent assay. According to the commercial DPT ELISA kit (product catalog) instructions. Set blank wells, standard wells and sample wells to be tested respectively. Add 100 μl of sample diluent to the blank well, add 100 μl of standard substance or test sample to the remaining wells, shake gently to mix, cover or cover the microtiter plate, and react at 37°C for 120 minutes. In order to ensure the validity of the experimental results, a new standard solution was used for each ex...

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Abstract

The invention discloses novel application of dermatopontin in preparing a kit for detecting cardiac failure and a detection kit. Through experimental verification, the DPT of the invention can be used to detect the cardiac failure. A method for rapidly detecting the DPT by using blood serum to estimate end-stage heart diseases in the invention has great clinical significance and popularization significance. The expression on the heat failure group DPT caused by the ARVC is up regulated by 3.5 times. The kit for detecting the cardiac failure, which is prepared by the DPT in the invention, has the advantages of high sensitiveness and specificity and high dilution rate of the blood serum. And compared with the conventional method for detecting the cardiac failure, the method of the inventionhas the advantages of low cost, high efficiency, convenient use and the like.

Description

technical field [0001] The invention relates to a new application of skin bridge protein (DPT), in particular to the application of skin bridge protein in the preparation of detection kits for heart failure. Background technique [0002] Dermatopontin (Dermatopontin, DPT) is a small molecular weight protein widely present in the extracellular matrix, which can interact with decorin, transforming growth factor β (TGF-β), type I collagen, etc. Functions such as cell adhesion and promotion of collagen fiber formation play a role in a variety of physiological and pathological processes related to the extracellular matrix. (Dermatopontin, a NovelPlayer in the Biology of the Extracellular Matrix. Okamoto O, Fujiwara S. Connective Tissue Research, 47:177-189, 2006.) [0003] The gene of skin bridge protein is located on human chromosome 1q12-1q23. It is a secreted protein consisting of 183 amino acid residues with a molecular weight of 22 kDa. The isoelectric points of different...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/53G01N33/543
Inventor 魏英杰胡盛寿刘晓艳张晓玲张浩郑哲张芃黄洁
Owner FUWAI HOSPITAL OF CARDIOVASCULAR DESEASE CHINESE ACAD OF MEDICAL SCI
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