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Tissue culture rapid breeding technology of betula luminfera leaf bud

A technology of tissue culture and tissue culture medium, which is applied in the field of rapid propagation of fine clone seedlings of Birch japonica, can solve the problems of different growth phenotypes, and it is difficult to achieve high population yields and the like.

Inactive Publication Date: 2010-08-11
中国林业科学研究院热带林业实验中心
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Problems solved by technology

At present, the birch glabra seedlings cultivated in my country are mainly seedlings, which have different growth phenotypes after afforestation due to the diversity of genetic materials, so it is difficult to achieve the goal of high yield in groups

Method used

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  • Tissue culture rapid breeding technology of betula luminfera leaf bud
  • Tissue culture rapid breeding technology of betula luminfera leaf bud
  • Tissue culture rapid breeding technology of betula luminfera leaf bud

Examples

Experimental program
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Embodiment Construction

[0021] exist figure 1 In this study, the selection and evaluation of excellent parent trees of Betula glabrata that reached the age of forest germplasm evaluation were carried out.

[0022] exist figure 2 In the process, the young leaf bud explants of the selected excellent mother tree were cut and collected with scissors.

[0023] exist image 3 In the process, the collected leaf buds were brought back to the laboratory for processing, and then disinfected with alcohol (concentration 75%) and mercury solution (concentration 3‰).

[0024] exist Figure 4 In this study, the effects of different basic medium components, different hormone types and concentrations on the induction and differentiation of leaf bud explant tissues were solved.

[0025] exist Figure 5 In the process, different media, hormone types and concentrations are used to germinate and root lateral shoots of tissue cultured seedlings at the same time to achieve the effect of proliferation.

[0026] exist...

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Abstract

The invention relates to a tissue culture rapid breeding technology of a betula luminfera leaf bud, which is a seed breeding technology for asexually expanding and breeding excellent individuals. The breeding technology is a biological technology developed according to the totipotency of plant cells. By the technical processes of selection of a betula luminfera superior tree, inducing differentiation of a superior tree leaf bud, proliferating, rooting culture and seedling transplantation, an excellent seedling with high yield and high efficiency can be rapidly cultured, and the single yield of wood of a forest land is improved.

Description

Technical field [0001] The patent of the invention belongs to forestry biotechnology, and is mainly applied to the rapid propagation of excellent clone seedlings of Betula glabra. Background technique [0002] Plant tissue culture is a biotechnology developed based on the totipotency of plant cells. It provides a set of advanced breeding methods for forest tree genetics and breeding. Its further application can better play the role of forest tree genetics and breeding in forestry production. At present, the seedlings of birch glabra cultivated in my country are mainly seedlings. Due to the diversity of genetic materials, seedlings have different growth phenotypes after afforestation, and it is difficult to achieve the purpose of high yield of the group. Clonal seedlings have the same phenotype after afforestation because of the same gene. A large number of excellent clone seedlings can be propagated by using the tissue culture rapid propagation technology of Betula glabra, ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 谌红辉
Owner 中国林业科学研究院热带林业实验中心