Method for establishing airway-free high-reactivity eosinophilic granulocyte airway inflammatory mouse model

A technology of airway hyperresponsiveness and eosinophils, applied in the field of applied basic medical research, can solve problems such as affecting judgment, research constraints, and few biochemical and immune reagents, and achieve the goal of reducing differences, wide application range, and good operating background Effect

Inactive Publication Date: 2010-08-11
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT) +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the model uses polymyxin nasal drops, which does not conform to the epidemiological situation of human EB
And the drug changed the internal state of the guinea pig, which may affect the final judgment
In addition, this model is not used for comparison with asthma, so it is not suitable for the study of the mechanism of airway hyperresponsiveness
Moreover, there are very few biochemical immune reagents for guinea pigs, and further research in the future is restricted.
Therefore, the application of this model is greatly limited, and no other studies have app

Method used

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  • Method for establishing airway-free high-reactivity eosinophilic granulocyte airway inflammatory mouse model
  • Method for establishing airway-free high-reactivity eosinophilic granulocyte airway inflammatory mouse model
  • Method for establishing airway-free high-reactivity eosinophilic granulocyte airway inflammatory mouse model

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Embodiment 1

[0021] (1) Animals and grouping: 30 SPF female Balb / c mice, 6-7 weeks old, weighing 18-20g, provided by Guangdong Experimental Animal Center (Purchase date: July 16, 2009; quality of experimental animals is qualified Proof: No.0052732), raised in the SPF animal room of Guangzhou Institute of Respiratory Diseases, free to eat and drink SPF feed and water, alternate day and night for 12 / 12 hours; randomly divided into normal saline (NS) control group and model Group and asthma group, 8 in each group.

[0022] (2) Reagents: chicken ovalbumin (OVA, grade V), aluminum hydroxide solution, methacholine (MCh), all purchased from Sigma Company in the United States, MCh excitation solution was prepared with normal saline, the concentration was 3.12mg / ml , 6.25mg / ml, 12.5mg / ml, 25mg / ml and 50mg / ml.

[0023] (3) Main instruments: Mouse pulmonary function instrument invasive detection system (RC System), American Buxco company; ordinary optical microscope, German ZEISS company.

[0024] (4) Mod...

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Abstract

The invention discloses a method for establishing an airway-free high-reactivity eosinophilic granulocyte airway inflammatory mouse model. The method comprises the following steps of: 1. preparation of a mouse: preparing a SPF (Specific Pathogen Free) level female Balb/c mouse with 5-6 week-ages; 2. molding of the mouse: respectively injecting 200mul of physiological saline solution mixed suspension of 10mug of chicken ovalbumin and 1.3mg of aluminum hydroxide into the abdominal cavity of the mouse at the 0th, the 7th and the 14th days of an experiment for carrying out sensitization; carrying out light anaesthesia on the mouse at the 21st, the 22nd and the 23rd days of the experiment, and carrying out nasal dripping excitation through 50mul of chicken ovalbumin solution with the mass percent concentration of 0.02 percent; and 3. treatment of the mouse: carrying out wounded lung function detection and bronchoalveolar lavage fluid cellular classification counting analysis on the mouse after the excitation of the last time for 24-48 hours, collecting the lung of the mouse to observe the histological change of the lung to obtain the hominine airway-free high-reactivity eosinophilic granulocyte airway inflammatory mouse model.

Description

Technical field [0001] The invention belongs to the field of applied basic medicine research, and particularly relates to a method for establishing an airway hyperresponsive eosinophilic airway inflammation mouse model. Background technique [0002] Asthma is a disease that seriously threatens human health worldwide. About 200 million people in our country suffer from asthma. If it cannot be controlled, it will seriously affect daily life and even cause life-threatening. Airway hyperresponsiveness and airway inflammation are the two main pathophysiological characteristics of asthma. Airway hyperresponsiveness is the severe obstructive response of the airway to various irritants. There are various stimuli, including direct stimuli and indirect stimuli. The environment and genes are all related to the airway hyperresponsiveness mechanism. Airway responsiveness testing has been widely used in the diagnosis and testing of asthma epidemiology and clinical research, and has become an...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61M31/00
Inventor 赖克方陈莉延钟南山谢佳星
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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