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Method for extracting total flavone from ampelopsis grossedentata

A technology of total flavonoids and vine tea, applied in the field of vine tea research, can solve the problems of cumbersome extraction process and low extraction rate, and achieve the effect of improving extraction rate, easy leaching, and strong selection

Inactive Publication Date: 2010-08-25
安徽省瑞森生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the extraction of total flavonoids mainly comes from plants of the genus Vitis genus, and the extraction rate is low or the extraction process is cumbersome.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Weigh 10 kg of dry leaves of vine tea, soak them in 180 L of 70% ethanol solvent for 120 min; filter the above alcohol solution, and after filtering, carry out microwave radiation treatment for 5 min with the wet tea leaves in a microwave oven, the microwave power is 800W, after the filter residue is subjected to microwave radiation treatment, Combine with the filtrate, add it to water at 98°C, and heat and extract at 98°C for 90 minutes; filter under reduced pressure, heat reflux to concentrate the filtrate, concentrate the filtrate to 1 / 3 of its volume, and then add water to it , crystallized and separated at 10°C to obtain yellow-brown total flavonoids with an extraction rate of 38.3%.

Embodiment 2

[0018] Weigh 10 kg of dry leaves of vine tea, soak in 150 L of 95% ethanol solvent for 90 min; filter the above alcohol solution, and after filtering, carry out microwave radiation treatment for 2 min with the wet tea leaves in a microwave oven, the microwave power is 800W, and after the filter residue is subjected to microwave radiation treatment, Combine with the filtrate, add it to water at 90°C, and heat and extract at 90°C for 60 minutes; filter under reduced pressure, heat reflux to concentrate the filtrate, concentrate the filtrate to 1 / 4 of its volume, and then add water to it , crystallized and separated at 10°C to obtain yellow-brown total flavonoids with an extraction rate of 35.9%.

Embodiment 3

[0020] Take by weighing 10 kg of dry leaves of rattan tea, soak in 160L of 50% ethanol solvent for 200 min; filter the above-mentioned alcohol solution, after filtering, carry out microwave radiation treatment for 5 min with the wet tea leaves in a microwave oven, the microwave power is 800W, after the filter residue is subjected to microwave radiation treatment, Combine with the filtrate, add it to water at 90°C, and heat and extract at 90°C for 120 minutes; filter under reduced pressure, heat reflux to concentrate the filtrate, concentrate the filtrate to 1 / 3 of its volume, and then add water to it , and crystallized at 5°C to obtain yellow-brown total flavonoids with an extraction rate of 36.3%.

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Abstract

The invention discloses a method for extracting total flavone from ampelopsis grossedentata. The method includes the following steps that: (1) dried ampelopsis grossedentata leaves are soaked in alcohol solvent for 1 to 5 hours; (2) the alcohol solution is filtered, filter residue is irradiated by microwaves, and are then merged in filtrate, and the filter residue and the filtrate are added into water with the temperature of 85 DEG C to 100 DEG C together, and are heated and extracted under the temperature of 85 DEG C to 100 DEG C for 50 to 140 minutes; (3) the extract is filtered and concentrated to one third to one fourth of the volume of the extract, water is then added into the extract, and crystallization takes place under the temperature of 0 DEG C to 10 DEG C. The adoption of alcohol assistance can remarkably increase the extraction rate, the extraction process adopting the microwave treatment technique is characterized by high efficiency, high selectivity and the like, moreover, operation is easy, byproduct is less, and the extraction rate of total flavone is as high as 38.3 percent.

Description

technical field [0001] The invention relates to the research field of vine tea, in particular to a method for extracting total flavonoids from vine tea. Background technique [0002] Vine tea (also known as wild vine tea, white tea, white tea cake), belongs to the grape family, the genus Snake Grape, and its scientific name is Snake Grape. It is a perennial vine and is also a very ancient traditional Chinese medicine resource and tea Plant resources and medicinal and edible plant resources. It is mainly distributed in Guangdong, Guangxi, Hainan, Yunnan, Guizhou, Hunan, Hubei, Jiangxi, Fujian and other provinces and regions, and grows in damp and fertile soils such as hillsides, forests, and river beaches at an altitude of 400-1300m. Compared with tea in the "Food Composition Table", its nutritional components are relatively complete. According to analysis, in vine tea, the crude protein content is 13.94%, the water-soluble protein content is 0.55%, the total amino acid cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/87A61P39/06A61P31/04A61P11/14A61P29/00A61P1/16A61P3/06A61P9/12A61P39/00A61P7/02A61P3/10A61K127/00
Inventor 韦洋向忠菊蔡菊刘小成
Owner 安徽省瑞森生物科技有限责任公司
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