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Method for enriching agaric polysaccharide in purified black agaric by using macroporous absorption resin

A technology of fungus polysaccharides and black fungus, which is applied to the purification of fungus polysaccharides, using macroporous adsorption resins to enrich and purify fungus polysaccharides in black fungus, which can solve the problems of low extraction purity and low extraction rate, and achieve high extraction purity and adsorption Fast, clear separation effect

Active Publication Date: 2010-08-25
HEILONGJIANG DINGHENGSHENG PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a method for enriching and purifying polysaccharides from black fungus using macroporous adsorption resins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: a kind of method utilizing macroporous adsorption resin to enrich and purify Schizandrin A in Schisandra chinensis, its steps are as follows:

[0027] (1) After crushing the dried black fungus, pass through a 60-mesh sieve, heat and reflux for extraction twice at a material-to-liquid ratio of 1:20, each time for 1 hour;

[0028] (2) high-speed centrifugation of the black fungus extract treated in (1), collecting the supernatant and filter residue respectively, and merging the filtrate to obtain the initial extract;

[0029] (3) Add an appropriate amount of water to the harvested initial extract, so that the weight of the mixed solution reaches 50 times the dry weight of the raw material, adjust its pH value to 3.0, then add the above filter residue to the mixed solution, heat to 40°C, and add pectin Enzyme (1 million u / ml) 1.0%, keep enzymatic hydrolysis for 3h after mixing. Cool to room temperature, let stand, filter, discard the filter residue, and conc...

Embodiment example 2

[0035] Example 2: A method for enriching and purifying polysaccharides from black fungus using macroporous adsorption resin, the steps are as follows:

[0036] (1) After crushing the dried black fungus, pass through a 10-mesh sieve, heat and reflux for extraction twice at a material-to-liquid ratio of 1:30, each time for 1 hour;

[0037] (2) high-speed centrifugation of the black fungus extract treated in (1), collecting the supernatant and filter residue respectively, and merging the filtrate to obtain the initial extract;

[0038] (3) Add an appropriate amount of water to the harvested initial extract, so that the weight of the mixed solution reaches 50 times the dry weight of the raw material, adjust its pH value to 5.0, then add the above filter residue to the mixed solution, heat to 50°C, and add pectin Enzyme (1 million u / ml) 1.5%, keep enzymatic hydrolysis for 3h after mixing. Cool to room temperature, let stand, filter, discard the filter residue, and concentrate the ...

Embodiment example 3

[0044] Example 3: A method for enriching and purifying polysaccharides from black fungus using macroporous adsorption resin, the steps are as follows:

[0045] (1) After crushing the dried black fungus, pass through an 80-mesh sieve, heat and reflux extraction twice with a material-to-liquid ratio of 1:20-50, each time for 1 hour;

[0046] (2) high-speed centrifugation of the black fungus extract treated in (1), collecting the supernatant and filter residue respectively, and merging the filtrate to obtain the initial extract;

[0047] (3) Add an appropriate amount of water to the harvested initial extract, so that the weight of the mixed solution reaches 50 times the dry weight of the raw material, adjust its pH value to 7.5, then add the above filter residue to the mixed solution, heat to 60°C, and add pectin Enzyme (1 million u / ml) 2.0%, keep enzymatic hydrolysis for 3h after mixing. Cool to room temperature, let stand, filter, discard the filter residue, and concentrate th...

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Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for enriching agaric polysaccharide in purified black agaric by using macroporous absorption resin. The invention is characterized in that a macroporous absorption technology is applied to separate the purified black agaric polysaccharide, the absorption selectivity to the agaric polysaccharide is good, the absorption and analysis are both fast, and the absorption capacity is larger; and the extraction is convenient and fast, the raw material source is rich, the production is low, the separating effect is obvious, the extracting purity is high, semi-finished products of agaric polysaccharide with the content of more than 35 percent and final products of agaric polysaccharide with the content of more than 98 percent can be obtained, and the defects that the conventional method has lower extraction rate and low extraction purity are overcome. The method selects macroporous absorption resin with stable physical-chemical properties, larger surface area, faster exchange velocity, high mechanical strength, strong anti-pollution capability and good heat stability, can absorb the agaric polysaccharide selectively from solution by physical absorption, and has fast absorption and analysis and larger absorption capacity.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for purifying polysaccharides from fungus, in particular to a method for enriching and purifying polysaccharides in black fungus by using a macroporous adsorption resin. Background technique: [0002] Black fungus: also known as light fungus. Mycological classification belongs to Basidiomycetes, Auricularia, Agaricaceae. It is distributed in Northeast China, Hubei, Zhejiang, and Yunnan. It is the production area of ​​Chinese fungus. It grows on the decayed wood of more than 120 kinds of broad-leaved trees such as oak, poplar, banyan, and locust tree, solitary or in groups. At present, artificial cultivation is mainly based on basswood and bag materials. The fungus fruiting body is gelatinous, disc-shaped, and the ear shape is irregular, with a diameter of 3-12 cm. Soft when fresh, horny when dry. It has a delicate taste and a special flavor. It is a well-known...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 姚德坤姚德利闫峰姚航
Owner HEILONGJIANG DINGHENGSHENG PHARM CO LTD
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