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Injectable bone growth factor-carrying microspheres and preparation method thereof

A technology of bone growth factor and growth factor, which is applied in the directions of medical preparations without active ingredients, medical preparations containing active ingredients, bone diseases, etc., can solve the problems of low bioavailability, diffusion dilution, etc., and achieves a simple preparation method. , Long shelf life, the effect of increasing half-life

Inactive Publication Date: 2010-09-01
SHENZHEN LANDO BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main difficulty in clinical application of BMP is that it is easy to diffuse and dilute in the body, and its bioavailability is extremely low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, preparation of osteogenic factor microspheres:

[0034] (1). Preparation of inner emulsion: Take 0.4 mL of BMP-2 solution with a concentration of 30 ug / ml into 4 mL of 20% PLGA (5 KDa) in dichloromethane, and homogenize until a uniform w / o emulsion is formed.

[0035] (2). Preparation of the external water phase: Take a certain amount of PVA solution into a certain amount of deionized water, stir at high speed to form a 0.1% solution.

[0036] (3). Preparation of w / o / w double emulsion: take the inner emulsion prepared in (1) and add the outer aqueous phase prepared in (2), and homogenize to form a uniform double emulsion. The ratio of internal emulsion to external aqueous phase is 1:15

[0037] (4). Solvent volatilization: continue to mechanically stir the double emulsion prepared in (3) for 2 hours to completely volatilize the dichloromethane.

[0038] (5). Freeze-drying the microsphere suspension prepared in (4) to obtain dry osteogenic factor microsph...

Embodiment 2

[0043] Embodiment 2, preparation of osteogenic factor microspheres:

[0044] (1). Preparation of inner emulsion: Take 0.4 mL of BMP-7 solution with a concentration of 10 ug / ml into 4 mL of 5% PLGA (100 KDa) in dichloromethane solution, and homogenize until a uniform w / o emulsion is formed.

[0045] (2). Preparation of the external water phase: Take a certain amount of PVA solution into a certain amount of deionized water, stir at high speed to form a 2% solution.

[0046] (3). Preparation of w / o / w double emulsion: take the inner emulsion prepared in (1) and add the outer aqueous phase prepared in (2), and homogenize to form a uniform double emulsion. The ratio of inner emulsion to outer aqueous phase is 1:5.

[0047] (4). Solvent volatilization: Continue to mechanically stir the double emulsion prepared in (3) for 4 hours to completely volatilize the chloroform.

[0048] (5). Freeze-drying the microsphere suspension prepared in (4) to obtain dry osteogenic factor microsphere...

Embodiment 3

[0053] Embodiment 3, preparation of osteogenic factor microspheres:

[0054] (1). Preparation of inner emulsion: Take 0.4 mL of BMP-1 solution with a concentration of 20 ug / ml into 4 mL of 5% PLA (10 KDa) in dichloromethane solution, and homogenize until a uniform w / o emulsion is formed.

[0055] (2). Preparation of the external water phase: Take a certain amount of PVA solution into a certain amount of deionized water, and stir at a high speed to form a 0.5% solution.

[0056] (3). Preparation of w / o / w double emulsion: take the inner emulsion prepared in (1) and add the outer aqueous phase prepared in (2), and homogenize to form a uniform double emulsion. The ratio of inner emulsion to outer aqueous phase is 1:10.

[0057] (4). Solvent volatilization: Continue to mechanically stir the double emulsion prepared in (3) for 3 hours to completely volatilize the dichloromethane.

[0058] (5). Freeze-drying the microsphere suspension prepared in (4) to obtain dry osteogenic factor...

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Abstract

The invention discloses microspheres which are used for curing osteoporosis and have a bone growth factor slow release function and injectablity, and a preparation method thereof. The bone growth factor-carrying microspheres are prepared by adopting a solution emulation-solvent volatilization method, and taking biodegradable polylactic acid or polyglycolic acid or poly lactic-co-glycolic acid as a starting material of the microspheres, wherein embedded bone growth factors are BMP-2 or BMP-7 or BMP-1; the average grain diameters of the prepared slow release microspheres are between 1.1 and 20.4 microns; and the release of the bone growth factors has a controllable slow release characteristic, and the release period thereof is 20 to 45 days.

Description

technical field [0001] The invention belongs to the field of medical equipment. More precisely, the present invention relates to a microsphere for treating osteoporosis, which has bone growth factor sustained-release function and injectability and a preparation method thereof. technical background [0002] Osteoporosis is a metabolic disease caused by endocrine, nutritional, immune, and genetic factors. According to the report of the World Health Organization, there are more than 200 million osteoporosis patients in the world, and it is a common and frequently-occurring disease. There are 75 million osteoporosis patients in the United States, Europe and Japan, and the annual cost of treatment and hospitalization is as high as 25 billion US dollars. Osteoporosis is recognized as the second largest health killer after cardiovascular disease. There are nearly 100 million osteoporosis patients in my country. At present, my country's social population has entered an aging peri...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61K9/10A61K47/42A61K47/34A61P19/10
Inventor 佘振定王明波谭荣伟
Owner SHENZHEN LANDO BIOMATERIALS
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