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Method for preparing iron nano-magnetic particles by taking T4 phage as template

A technology of magnetic particles and bacteriophages, applied in the directions of viruses/bacteriophages, biochemical equipment and methods, magnetic materials, etc., can solve the problems of limiting the application of Fe nanoparticles, irregular particle morphology, and lack of ferromagnetism, and achieve large-scale Large-scale production, uniform distribution, small particle size effect

Inactive Publication Date: 2010-09-01
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

People also use iron oleate as a precursor to obtain Fe nanoparticles through thermal degradation, but the prepared particles have irregular morphology, relatively large particle size, and do not have ferromagnetic properties, which limits the magnetic properties of Fe nanoparticles. Applications in the Field of Recording Materials

Method used

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  • Method for preparing iron nano-magnetic particles by taking T4 phage as template
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  • Method for preparing iron nano-magnetic particles by taking T4 phage as template

Examples

Experimental program
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Effect test

Embodiment 1

[0020] 1. Amplification and purification of T4 phage

[0021] First, insert T4 bacteriophage strains into 50ml of pre-incubated Escherichia coli suspension with a concentration of 10mg / ml, and culture at 37°C on a shaking table at a speed of 100r / min for 8h. Centrifuge the suspension containing Escherichia coli residual shell and T4 phage at 4°C at speeds of 4,000, 5,000, and 6,000 r / min, respectively, for 15 minutes each time, remove the precipitate, and take the supernatant. Finally, ultracentrifuge the pure T4 phage liquid at 4°C, 45000r / min for 2.5h, remove the supernatant, and disperse the T4 phage pellets into 2ml of deionized water.

[0022] 2. Preparation of Fe nanomagnetic particles using T4 phage as template

[0023] Take 300 ul of the enriched T4 phage liquid, adjust the pH value to 8.5 with 1 mol / l NaOH, and incubate on a shaking table at 4°C and 60 r / min for 3 hours. Take 300ul of FeCl with a concentration of 5mM 3 The solution was added to the above-mentioned ...

Embodiment 2

[0025] 1. Amplification and purification of T4 phage

[0026] First, insert T4 phage strains into 50 ml of pre-incubated Escherichia coli suspension with a concentration of 12 mg / ml, and culture at 37° C. on a shaking table at a speed of 130 r / min for 12 hours. Centrifuge the suspension containing Escherichia coli residual shell and T4 phage at 10°C at speeds of 4,000, 5,000, and 6,000 r / min, respectively, for 17 minutes each time, remove the precipitate, and take the supernatant. Finally, ultracentrifuge the pure T4 phage liquid at 10°C, 42100r / min for 3h, remove the supernatant, and disperse the T4 phage pellets into 2ml of deionized water.

[0027] 2. Preparation of Fe nanomagnetic particles using T4 phage as template

[0028] Take 400 ul of the enriched T4 phage liquid, adjust the pH value to 8.5 with 1 mol / l NaOH, and incubate at 10°C and 90 r / min on a shaking table for 4 hours. Take 400ul of FeCl with a concentration of 10mM 3The solution was added to the above-mentio...

Embodiment 3

[0030] 1. Amplification and purification of T4 phage

[0031] First, insert T4 bacteriophage strains into 50 ml of pre-incubated Escherichia coli suspension at a concentration of 15 mg / ml, and culture at 37° C. on a shaking table at a speed of 150 r / min for 15 hours. Centrifuge the suspension containing Escherichia coli residual shell and T4 phage at 6°C at speeds of 4,000, 5,000, and 6,000 r / min, respectively, for 20 minutes each time, remove the precipitate, and take the supernatant. Finally, ultracentrifuge the pure T4 phage liquid at 6°C and 40,000 r / min for 3 hours, remove the supernatant, and disperse the T4 phage pellets into 2 ml of deionized water.

[0032] 2. Preparation of Fe nanomagnetic particles using T4 phage as template

[0033] Take 500 ul of the enriched T4 phage liquid, adjust the pH value to 9.0 with 1 mol / l NaOH, and incubate at 6° C. for 5 h on a shaker at 110 r / min. Take 500ul of FeCl with a concentration of 10mM 3 The solution was added to the above-...

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Abstract

The invention discloses a method for preparing iron nano-magnetic particles by taking a T4 phage as a template in the field of magnetic recording materials. In the method, the T4 phage obtained by enrichment and the solution of iron chloride are incubated, centrifuged and reduced by utilizing intrinsic structural characteristics of an organism and a molecular recognition function thereof to obtain iron nano-particles which have small particle sizes and high dispersion degrees and are regularly distributed on the capsid surface of the T4 phage, and the prepared iron nano-particles have the characteristics of nonreversible magnetization process, high coercive force and the like, so that the iron nano-particles prepared by the method have wide application prospect in the field of high-intensity magnetic recording materials. Moreover, in the method, a biological nano-structure which exists in nature is directly taken as the template, so that the method has simple preparation processes, moderate reaction conditions and environment friendliness. Since a biological template has the characteristics of self-producing capability, high appearance repeatability and the like, large scale production is easy to realize.

Description

technical field [0001] The invention belongs to the field of magnetic recording materials, and in particular relates to a method for preparing iron nanoparticles with small diameter and ferromagnetism by using T4 bacteriophage as a template. Background technique [0002] Transition metal iron (Fe) nanoparticles have the characteristics of small size, single magnetic domain structure, and high coercive force. The magnetic recording materials made with it have the advantages of good stability, clear image, high signal-to-noise ratio, and low distortion. It is because of these that nano-magnetic materials have broad application prospects in the field of high-density magnetic recording materials. However, with the development of information technology, the amount of information that needs to be recorded is also increasing. Therefore, the requirements for magnetic recording materials are getting higher and higher, and the high performance of recording materials is required, espec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24H01F1/047C12N7/00
Inventor 高发明侯莉孙红敬
Owner YANSHAN UNIV
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