Detection kit for vibrio cholerae O1 group and detection method thereof

Abstract

The invention relates to a detection kit for a vibrio cholerae O1 group, which comprises BstDNA polymerase, reaction liquid, sample pretreatment liquid, coloration liquid, stabilizing solution and positive control, and also comprises two pairs of primers which take vibrio cholerae RfbN genes as target genes and two pairs of primers which are designed based on the loop-mediated isothermal amplification technology, namely inner primers FIP / BIP and outer primers F3 / B3. The detection kit for the vibrio cholerae O1 group has the advantages of comprehensive detection effect, high specificity and low omission factors, and is suitable for the quick detection of the vibrio cholerae O1 group.

Description

technical field

[0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a detection kit for Vibrio cholerae O1 group and a detection method thereof.

[0002] Background technique

[0003] Cholera is a severe infectious disease with diarrhea as the main symptom. So far, seven world pandemics have occurred. The seventh world pandemic of cholera, caused by Vibrio cholerae Eltor, began in 1961 and affected 140 countries and regions. More than 4 million cases were reported. According to the WHO expert meeting, there are about 5.5 million cases worldwide each year, among which Asia, Africa and Latin America are more serious, causing 100,000 deaths in Asia and 20,000 deaths in Africa. To this day, cholera remains one of the most dangerous infectious diseases. Therefore, early rapid and correct diagnosis is of great significance to the treatment and prevention of the spread of this disease.

[0004] At present, Vibrio cholerae has...

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