High-efficiency mixing renaturation device beneficial to rPA (radar Performance Analyzer) large-scale renaturation

A large-scale and high-efficiency technology, which can be used in biochemical cleaning devices, enzymology/microbiology devices, bioreactor/fermenter combinations, etc. , to achieve the effect of improving the renaturation rate, improving the renaturing rate and easy operation

Active Publication Date: 2010-09-15
EAST CHINA UNIV OF SCI & TECH +1
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  • Application Information

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Problems solved by technology

[0008] The present invention provides a high-efficiency mixing renaturation device that is beneficial to rPA large-scale renaturation, so as to solve the technical problems in the above-mentioned background technology, that is, the shear force generated by flat-leaf pulp is large, which easily leads to aggregation of denatured proteins and Denaturation and inactivation of refolding proteins

Method used

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  • High-efficiency mixing renaturation device beneficial to rPA (radar Performance Analyzer) large-scale renaturation
  • High-efficiency mixing renaturation device beneficial to rPA (radar Performance Analyzer) large-scale renaturation
  • High-efficiency mixing renaturation device beneficial to rPA (radar Performance Analyzer) large-scale renaturation

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Embodiment 1100

[0027] Example 1100L-scale high-efficiency mixing and renaturation device

[0028] figure 1 It is a schematic diagram of the first embodiment of the present invention, as shown in the figure, in this embodiment:

[0029] 1. Propeller: Single-layer propeller is used to reduce shear force.

[0030] 2. The liquid inlet pipe for adding denatured protein into the refolding solution: the liquid outlet adopts a single-tube multi-nozzle design, and all the nozzles are below the liquid level to ensure that the denatured protein is quickly and evenly added to the refolding solution to avoid excessive local instantaneous concentration. high to produce aggregated precipitates.

[0031] Implementation effect: Compared with conventional renaturation devices, this device can increase the renaturation rate by more than 25%.

Embodiment 2300

[0032] Example 2300L-scale high-efficiency mixing and renaturation device

[0033] figure 2 It is a schematic diagram of the second embodiment of the present invention, as shown in the figure, in this embodiment:

[0034] 1. Propeller: Double-layer propeller is used to reduce shear force.

[0035] 2. The liquid inlet pipe for adding denatured protein into the refolding solution: the liquid outlet adopts a double-tube multi-nozzle design, and all the nozzles are below the liquid level to ensure that the denatured protein is quickly and evenly added to the refolding solution to avoid excessive local instantaneous concentration resulting in aggregated precipitation.

[0036] Implementation effect: Compared with conventional renaturation devices, this device can increase the renaturation rate by more than 22%.

Embodiment 3500

[0037] Example 3500L-scale high-efficiency mixing and renaturation device

[0038] The high-efficiency mixed refolding device is shown in the attached image 3 , the key structure description is as follows:

[0039] 1. Propeller: Three-layer propeller is used to reduce shear force.

[0040] 2. The liquid inlet pipe for adding denatured protein into the refolding solution: the liquid outlet adopts a three-pipe multi-nozzle design, and all the nozzles are below the liquid level, so as to ensure that the denatured protein is quickly and evenly added to the refolding solution to avoid excessive local instantaneous concentration resulting in aggregated precipitation.

[0041] Implementation effect: Compared with conventional renaturation devices, this device achieves an increase of more than 20% in renaturation rate.

[0042] Adding the denatured protein used in the large-scale renaturation of the rPA inclusion body of the present invention into the mixing device can effectively...

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PUM

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Abstract

The invention relates to a high-efficiency mixing renaturation device which is beneficial to rPA large-scale renaturation and is used for adding a denatured protein solution into a renaturation buffer solution. The device comprises a container, a liquid inlet pipe and a propeller, wherein the renaturation buffer solution is loaded in the container; the liquid inlet pipe is provided with a plurality of nozzles, one end of the liquid inlet pipe is a liquid outlet end, the other end is a liquid inlet end, and the denatured protein solution is introduced into the liquid inlet end; and the propeller is inserted into a position below the liquid level of the renaturation buffer solution. Therefore, the invention can effectively inhabit the agglutination reaction of an rPA inclusion body when in renaturation and markedly improve the renaturation ratio of target protein.

Description

technical field [0001] The invention belongs to the field of biopharmaceutical engineering, in particular to a high-efficiency mixing device for improving the renaturation rate of recombinant human tissue plasminogen kinase derivatives (rPA) in large-scale renaturation. Background technique [0002] Acute myocardial infarction is a disease that seriously endangers human health, and the mortality rate is extremely high. According to statistics, more than 1 million people are killed by acute myocardial infarction every year. Epidemiological surveys show that the incidence of acute myocardial infarction in China is about 5 / 10,000, and heart disease ranks second as the cause of death among Chinese residents. Thrombolytic therapy has become a recognized and effective method for the treatment of acute myocardial infarction because of its fast, simple, economical, and easy-to-operate characteristics, and it has become the main method for treating acute myocardial infarction in my ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/02
CPCC12M29/06C12M27/02
Inventor 秦玉峰尤金花张元兴周祥山
Owner EAST CHINA UNIV OF SCI & TECH
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