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High-efficiency mixing renaturation device beneficial to rPA (radar Performance Analyzer) large-scale renaturation

A large-scale and high-efficiency technology that can be used in biochemical cleaning devices, enzymology/microbiology devices, bioreactor/fermenter combinations, etc. , to achieve the effect of improving the renaturation rate, improving the renaturing rate and easy operation

Active Publication Date: 2013-02-06
EAST CHINA UNIV OF SCI & TECH +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention provides a high-efficiency mixing renaturation device that is beneficial to rPA large-scale renaturation, so as to solve the technical problems in the above-mentioned background technology, that is, the shear force generated by flat-leaf pulp is large, which easily leads to aggregation of denatured proteins and Denaturation and inactivation of refolding proteins

Method used

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  • High-efficiency mixing renaturation device beneficial to rPA (radar Performance Analyzer) large-scale renaturation
  • High-efficiency mixing renaturation device beneficial to rPA (radar Performance Analyzer) large-scale renaturation
  • High-efficiency mixing renaturation device beneficial to rPA (radar Performance Analyzer) large-scale renaturation

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Embodiment 1100

[0027] Example 1100L-scale high-efficiency mixed renaturation device

[0028] figure 1 It is a schematic diagram of the first embodiment of the present invention. As shown in the figure, in this embodiment:

[0029] 1. Propeller: Single-layer propeller is adopted to reduce shearing force.

[0030] 2. The liquid inlet pipe for adding denatured protein to the renaturing liquid: the liquid outlet is designed with a single tube and multiple nozzles, and the nozzles are all below the liquid surface, which ensures that the denatured protein is added into the renaturing liquid quickly and evenly, and avoids local instantaneous excessive concentration. high, resulting in aggregated precipitation.

[0031] Implementation effect: Compared with the conventional renaturation device, the renaturation rate of this device is increased by more than 25%.

Embodiment 2300

[0032] Example 2300L-scale high-efficiency mixed renaturation device

[0033] figure 2 It is a schematic diagram of the second embodiment of the present invention. As shown in the figure, in this embodiment:

[0034] 1. Propeller: Double-layer propeller is adopted to reduce shearing force.

[0035] 2. The liquid inlet pipe for adding denatured protein to the renaturing liquid: the liquid outlet adopts a double-pipe multi-nozzle design, and the nozzles are all below the liquid level to ensure that the denatured protein is added into the renaturing liquid quickly and evenly, avoiding local instantaneous high concentration. resulting in aggregated precipitates.

[0036] Implementation effect: Compared with the conventional renaturation device, the renaturation rate of this device is increased by more than 22%.

Embodiment 3500

[0037] Example 3500L-scale high-efficiency mixed renaturation device

[0038] The high-efficiency mixing and renaturing device is shown in the attached image 3 , the key structure is described as follows:

[0039] 1. Propeller: Three-layer propeller is adopted to reduce shearing force.

[0040] 2. The inlet pipe for adding denatured protein to the renaturing solution: the liquid outlet adopts a three-tube multi-nozzle design, and the nozzles are all below the liquid level, to ensure that the denatured protein is added into the renaturing solution quickly and evenly, avoiding local instantaneous high concentration resulting in aggregated precipitates.

[0041] Implementation effect: Compared with the conventional renaturation device, the renaturation rate of this device is increased by more than 20%.

[0042] The denatured protein used in the large-scale renaturation of the rPA inclusion body of the present invention is added to the mixing device, which can effectively inhi...

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PUM

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Abstract

The invention relates to a high-efficiency mixing renaturation device which is beneficial to rPA large-scale renaturation and is used for adding a denatured protein solution into a renaturation buffer solution. The device comprises a container, a liquid inlet pipe and a propeller, wherein the renaturation buffer solution is loaded in the container; the liquid inlet pipe is provided with a plurality of nozzles, one end of the liquid inlet pipe is a liquid outlet end, the other end is a liquid inlet end, and the denatured protein solution is introduced into the liquid inlet end; and the propeller is inserted into a position below the liquid level of the renaturation buffer solution. Therefore, the invention can effectively inhabit the agglutination reaction of an rPA inclusion body when in renaturation and markedly improve the renaturation ratio of target protein.

Description

technical field [0001] The invention belongs to the field of biopharmaceutical engineering, in particular to a high-efficiency mixing device which is beneficial to the large-scale renaturation of recombinant human tissue-type plasminogen kinase derivatives (rPA). Background technique [0002] Acute myocardial infarction is a disease that seriously endangers human health and has a very high mortality rate. According to statistics, more than 1 million people are killed by acute myocardial infarction every year. Epidemiological surveys show that the incidence of acute myocardial infarction in China is about 5 / 10,000, and heart disease is the second leading cause of death among Chinese residents. Thrombolytic therapy has become a recognized and effective method for the treatment of acute myocardial infarction because of its fast, simple, economical and easy-to-operate characteristics. Therefore, the research and development of high-efficiency, safe, and less adverse effects of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/02
CPCC12M27/02C12M29/06
Inventor 秦玉峰尤金花张元兴周祥山田守生解福生郝向慧刘海峰庞甲佩
Owner EAST CHINA UNIV OF SCI & TECH
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