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Preparation method of chitosan/sodium polyphosphate porous microcarrier

A technology of sodium polyphosphate and chitosan acid, which is applied in the preparation of microspheres, microcapsule preparations, animal cells, etc., can solve problems such as unevenness, poor controllability, and uncontrollable particle size, and achieve roundness The effect of high density and uniform particle size distribution

Inactive Publication Date: 2010-09-29
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the preparation method of chitosan / sodium polyphosphate porous microcarrier is the emulsification method, which is not controllable, and the roundness of the formed microspheres is not good, and the particle size is uncontrollable and uneven.

Method used

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  • Preparation method of chitosan/sodium polyphosphate porous microcarrier
  • Preparation method of chitosan/sodium polyphosphate porous microcarrier
  • Preparation method of chitosan/sodium polyphosphate porous microcarrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of preparation method of chitosan / sodium polyphosphate porous micro-carrier high-pressure pulse into balls, comprising the following steps:

[0036] (1), solution preparation

[0037] Preparation concentration is 2.5% chitosan acid solution and pH is 10 sodium polyphosphate aqueous solution;

[0038] Wherein the reaction ratio of chitosan acid solution and sodium polyphosphate aqueous solution is calculated by volume, i.e. chitosan acid solution: sodium polyphosphate aqueous solution is 2: 1;

[0039] The chitosan deacetylation degree ≥ 90%;

[0040] (2), constant temperature oscillation

[0041] Oscillate the 2.5% chitosan acid solution obtained in step (1) in a constant temperature oscillator, control the temperature of the water bath at 37° C., and leave it to defoam after 24 hours of constant temperature;

[0042] (3), high-voltage pulse into a ball

[0043] Step (2) After constant temperature oscillation and defoaming, the concentration is 2.5% chitosan ...

Embodiment 2

[0054] A kind of preparation method of chitosan / sodium polyphosphate porous micro-carrier high-pressure pulse into balls, comprising the following steps:

[0055] (1), solution preparation

[0056] Preparation concentration is 2% chitosan acid solution and pH is 10 sodium polyphosphate aqueous solution;

[0057] Wherein the reaction ratio of chitosan acid solution and sodium polyphosphate aqueous solution is calculated by volume, i.e. chitosan acid solution: sodium polyphosphate aqueous solution is 2: 1;

[0058] The chitosan deacetylation degree ≥ 90%;

[0059] (2), constant temperature oscillation

[0060] Oscillate the 2% chitosan acid solution obtained in step (1) in a constant temperature oscillator, control the temperature of the water bath at 37° C., and leave it to defoam after 24 hours of constant temperature;

[0061] (3), high-voltage pulse into a ball

[0062] Step (2) After constant temperature oscillation and defoaming, the concentration is 2% chitosan acid sol...

Embodiment 3

[0072] A kind of preparation method of chitosan / sodium polyphosphate porous micro-carrier high-pressure pulse into balls, comprising the following steps:

[0073] (1), solution preparation

[0074] Preparation concentration is 3% chitosan acid solution and pH is 10 sodium polyphosphate aqueous solution;

[0075] Wherein the reaction ratio of chitosan acid solution and sodium polyphosphate aqueous solution is calculated by volume, i.e. chitosan acid solution: sodium polyphosphate aqueous solution is 2: 1;

[0076] The chitosan deacetylation degree ≥ 90%;

[0077] (2), constant temperature oscillation

[0078] Vibrate the 3% chitosan acid solution obtained in step (1) in a constant temperature oscillator, control the temperature of the water bath at 37° C., and leave it to defoam after 24 hours of constant temperature;

[0079] (3), high-voltage pulse into a ball

[0080] Step (2) After constant temperature oscillation and defoaming, the concentration is 3% chitosan acid sol...

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PUM

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Abstract

The invention discloses a preparation method of chitosan / sodium polyphosphate porous microcarrier, which comprises the following steps: dropping chitosan into sodium polyphosphate solution under high pressure through a high-pressure pulse microcapsule forming device to form microspheres, cross-linking the microspheres with glutaric dialdehyde, and freezing-drying, thereby forming the chitosan / sodium polyphosphate porous microcarrier of a three-dimensional structure. The chitosan / sodium polyphosphate porous microcarrier prepared in the method has the advantages of good roundness and uniform particle size distribution. In addition, due to the adoption of the freezing-drying technology under the condition that the pH of the sodium polyphosphate is ensured during the preparation of the chitosan / sodium polyphosphate porous microcarrier, the particle size of the prepared chitosan / sodium polyphosphate porous microcarrier is 15-20 mum, the porosity thereof is close to 90 percent, and the water absorbing capacity thereof is up to 8 times.

Description

technical field [0001] The invention relates to a preparation method of a chitosan / sodium polyphosphate porous microcarrier, in particular to a preparation method of a chitosan / sodium polyphosphate porous microcarrier formed into balls by high-voltage pulses. Background technique [0002] Microcarrier culture animal cell technology can obtain a large number of cells in a short period of time, and the cell passage only needs to add new microcarriers, which can basically avoid the damage of cells during the trypsinization process. Therefore, it is very convenient and meaningful to culture cells with microcarriers. [0003] At this stage, whether it is laboratory or industrial production, the cell culture microcarriers used are mainly solid Cytodex series carriers. This kind of carrier uses dextran as the matrix, and the surface is covered with gelatin, which is expensive, and the mechanical strength and particle elasticity of the matrix dextran are not very ideal. More impor...

Claims

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Application Information

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IPC IPC(8): B01J13/04C12N5/07
Inventor 周燕刘宝林韩宝三杨晓昀王欣杨波彭承宏
Owner UNIV OF SHANGHAI FOR SCI & TECH
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