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Method for preparing chondroitin sulfates

A chondroitin sulfate and pretreatment technology, applied in the field of bioengineering, can solve the problems of long production cycle and high cost, and achieve the effects of improving the crushing speed, reducing the cost and improving the extraction efficiency.

Inactive Publication Date: 2012-07-04
CHENGDU FANXINJIA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, dozens of pharmaceutical factories in China are producing ChS, which generally adopt dilute alkali method and concentrated alkali method, and trypsin is used, resulting in long production cycle and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A. Pretreatment: boil the pork throat bone in boiling water for 8 minutes, remove the fat and meat residue, and cut it into 1.5cm square pieces;

[0021] B. Extraction: Submerge 500 g of the pretreated laryngeal bone into 3000 ml of 20 g / l NaOH solution, place it in an ultrasonic cleaner for ultrasonic treatment for 1.2 hours, and filter it with gauze to obtain the filtrate;

[0022] C. Ultrasonic impurity removal: the filtrate was ultrasonically treated for another 6 hours to remove impurities such as protein to obtain a treatment solution;

[0023] D. Decolorization: adjust the pH of the treatment solution to 7.5, add kaolin according to 9.5% of the dry weight of the throat bone, add an equal weight of activated activated carbon according to the volume of 4% of the treatment solution, quickly heat up to 90°C for 1 hour, and after rapid cooling, suction filtration get the filtrate;

[0024] E. Precipitation: add equal weight NaCl according to 1% of the volume of the f...

Embodiment 2

[0027] A. Pretreatment: boil the pork throat bone in boiling water for 13 minutes, remove the fat and meat residue, and cut it into 2.5cm square pieces;

[0028] B. Extraction: Submerge 350 g of the pretreated larynx into 2450 ml of 20 g / l NaOH solution, place it in an ultrasonic cleaner for ultrasonic treatment for 2.5 hours, and filter it with gauze to obtain the filtrate;

[0029] C. Ultrasonic impurity removal: ultrasonically treat the filtrate for another 2 hours to remove impurities such as protein to obtain a treatment liquid;

[0030] D. Decolorization: adjust the pH of the treatment solution to 7, add kaolin according to 7.8% of the dry weight of the throat bone, add an equal weight of activated activated carbon according to the volume of the treatment solution 4.2%, quickly heat up to 90°C for 2 hours, and after rapid cooling, suction filtration get the filtrate;

[0031] E. Precipitation: add equal weight NaCl according to 1% of the volume of the filtrate, filter w...

Embodiment 3

[0034] A. Pretreatment: boil the pork throat bone in boiling water for 6 minutes, remove the fat and meat residue, and cut it into 1cm square pieces;

[0035] B. Extraction: Submerge 620 g of the pretreated laryngeal bone into 3100 ml of 20 g / l NaOH solution, place it in an ultrasonic cleaner for ultrasonic treatment for 3 hours, and filter it with gauze to obtain the filtrate;

[0036] C. Ultrasonic impurity removal: the filtrate is ultrasonically treated for another 7 hours to remove impurities such as protein to obtain a treatment solution;

[0037] D. Decolorization: adjust the pH of the treatment solution to 6.2, add kaolin according to 11% of the dry weight of the throat bone, add an equal weight of activated activated carbon according to the volume of 0.9% of the treatment solution volume, quickly heat up to 90°C for 1.8h, and after rapid cooling, pump filtrate;

[0038] E. Precipitation: add equal weight NaCl according to 1% of the volume of the filtrate, filter with ...

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Abstract

The invention relates to a method for preparing chondroitin sulfates, and belongs to the field of bioengineering. An ultrasonic method is adopted and comprises technical steps of: pretreatment, extraction, ultrasonic impurity removal, decoloration, precipitation, dehydration, and the like. The method has the advantages of retaining bioactivity, improving crushing speed, reducing crushing time, improving extraction efficiency greatly, and reducing cost.

Description

technical field [0001] The invention relates to a preparation method of chondroitin sulfate, which belongs to the field of bioengineering. Background technique [0002] Chondroitin Sulfate (ChS) is widely distributed in tissues (such as cartilage, umbilical cord, blood vessel walls, etc.) of humans and some animals such as pigs, cattle, sheep, and fish, and its main component is 4-ChS (ChS A) And 6-ChS (ChS C) and other isomer mixtures. [0003] Chondroitin sulfate is an acidic mucopolysaccharide with strong water absorption. It is a white powder, easily soluble in water to form a solution with high viscosity, and insoluble in organic solvents such as ethanol and acetone. Domestically, it is extracted and prepared from cartilage tissues such as pig's throat bone, nasal middle bone, and trachea. It is used for certain nervous headaches, neuralgia, arthralgia, migraine, arteriosclerosis, etc., and can also be used for hearing impairment caused by streptomycin and adjuvant th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
Inventor 谭晶
Owner CHENGDU FANXINJIA TECH
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