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Alcohol preparation method by fermenting cane juice with foamed ceramic immobilized yeast

A technology for immobilizing yeast and foam ceramics, applied in biochemical equipment and methods, microorganism-based methods, immobilization on or in inorganic carriers, etc. The yeast activation time is long and the non-production cycle is prolonged to achieve the effect of improving equipment utilization, saving production time and not easy to contaminate

Inactive Publication Date: 2012-05-23
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cheng Jiangfeng and He Guoqing's research "Study on the Preparation Process of Honeycomb Ceramic Immobilized Yeast Cell Biocatalyst" (Food Science, 2003, 24(5): 91-94), the number of immobilized yeast cells was 10 6 ~10 7 cfu / unit carrier, used for continuous main fermentation of beer (Journal of Process Engineering, 2003, 3(5): 419-422), which saves the cost of yeast, but due to the low pore density of honeycomb ceramics, the amount of yeast adsorbed is small, The activation time of immobilized yeast is longer, which prolongs the non-production period. Due to the prolongation of the start-up fermentation time, the plan of contaminating miscellaneous bacteria such as lactic acid bacteria and acetic acid bacteria is increased, and the production efficiency is reduced.

Method used

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  • Alcohol preparation method by fermenting cane juice with foamed ceramic immobilized yeast
  • Alcohol preparation method by fermenting cane juice with foamed ceramic immobilized yeast
  • Alcohol preparation method by fermenting cane juice with foamed ceramic immobilized yeast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] In the first step, in terms of weight percentage, the Angel Super Brewing high-activity dry yeast powder (sugar raw material) was added to the first sugarcane juice medium of 6°BX with pH 4.0 at 0.1%, and cultured at 20°C, 150rpm 12h, it becomes the yeast seed liquid; the raw material formula of the first sugarcane juice medium is: peptone 5.0kg, yeast extract 2.0kg, diammonium citrate 1.0kg, dipotassium hydrogen phosphate 1.0kg, sugarcane juice 700kg, production Drink 291kg of drinking water, adjust the pH to 4.0 with 1.0mol / L sulfuric acid solution; stir and dissolve evenly, sterilize it with a plate heat exchanger under 0.4bar steam, keep it at 110°C for 10s, and cool to 20°C for standby; Angel Super brewing high-activity dry yeast powder is produced by Angel Yeast Co., Ltd. It has the characteristics of acid tolerance (pH 2.5), ethanol tolerance (>18%v / v), sugar tolerance (60% glucose), strong reproduction ability, and rapid main fermentation. .

[0032] In the second...

Embodiment 2

[0039] In the first step, the Angel Super Brewing High Active Dry Yeast Powder (carbohydrate raw material, same as in Example 1) was added to the first sugarcane juice medium at 10°BX with pH 6.0 at 1.0% in weight percentage. Cultivate at 300 rpm for 6 hours to become seed liquid; the first sugarcane juice medium formula is: peptone 20kg, yeast extract 10kg, diammonium citrate 5kg, dipotassium hydrogen phosphate 5kg, sugarcane juice 700kg, production and drinking Water 260kg, adjust pH to 6.0 with 0.1mol / L sulfuric acid solution; stir and dissolve evenly, use plate heat exchanger to sterilize under 0.6bar steam, keep at 140℃ for 5s, and cool to 30℃ for standby;

[0040] In the second step, in terms of volume percentage, 10% of the yeast seed liquid was inserted into the 20°BX mother starter medium, and cultured at 35°C and 300rpm for 6 hours to become the mother starter yeast suspension; the mother fermentation The medium formula is: 20kg of peptone, 10kg of yeast extract, 5kg of...

Embodiment 3

[0046] In the first step, in terms of weight percentages, the Angel Super Brewing High Active Dry Yeast Powder (sugar raw material, same as in Example 1) was added at 0.5% into a 9°BX sugarcane juice medium with pH 5.0 at 25.0°C, Cultivate for 9 hours at 175rpm to become seed liquid; in parts by weight, the sugarcane juice medium formula is: peptone 12kg, yeast extract 6kg, diammonium citrate 3kg, dipotassium hydrogen phosphate 3kg, sugarcane squeezed juice 600kg, Produce 376kg of drinking water, adjust pH to 5.0 with 0.5mol / L sulfuric acid solution; stir and dissolve evenly, sterilize with plate heat exchanger under 0.5bar steam, keep at 125℃ for 7.0s, and cool to 25℃ for standby;

[0047] In the second step, in terms of volume percentage, insert the yeast seed liquid into the 15°BX mother starter medium at 6%, and incubate at 27.5°C and 175rpm for 9 hours to become the mother starter yeast suspension; in parts by weight In total, the mother starter culture medium formula is: pe...

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Abstract

The invention discloses an alcohol preparation method by fermenting cane juice with foamed ceramic immobilized yeast, comprising the following steps: enlarging the cultivation of saccharomyces cerevisiae powder by the 'two-step method' to ensure the cells are in synchronous growth period to the greatest extent, then soaking and washing the foamed ceramic with clean water, drying, then soaking the foamed ceramic completely in yeast suspension of mother culture to absorb for 1-4h, taking out the foamed ceramic which is through adsorption to be used in production; or drying at 40-55 DEG C, adding the foamed ceramic through adsorption in cane juice culture medium in a ratio of 1:2-5 for multiplication, controlling the pH to 3.0-5.0 to culture for 10-15h, inoculating 3-5% of fermented liquor in cane juice sugar solution to perform continuous fermentation at 25-35 DEG C for 24-40h, and pumping fermentation liquor to a distillation column for distillation, wherein, the content of alcohol is 12-18%. The invention saves the time for enlarging the cultivation of yeast and shortens the production cycle of alcohol.

Description

Technical field [0001] The invention relates to a method for preparing alcohol by fermenting sugarcane juice, in particular to a method for preparing alcohol by fermenting sugarcane juice with foam ceramic immobilized yeast. Background technique [0002] Chinese invention patent 200710032548.4 (CN 101225407A) discloses "a method for producing alcohol by fermenting sugarcane as a raw material with immobilized enzymes". In this method, sugarcane (cut into 1 to 2cm) is used as a carrier, and the effect is very good in the laboratory, but Because sugarcane is too short, when used in large-scale production, it is easy to accumulate and affect the mass transfer of mash, and ultimately affect the fermentation yield. Chinese invention patent 200510041888.4 (CN 1837370A) discloses "the production method of immobilized yeast cells for alcohol fermentation". The method uses polyvinyl alcohol (PVA) as the main raw material, and polyvinyl alcohol is a chemical raw material that can be inhaled...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12N11/14C12R1/865
CPCY02E50/17Y02E50/10
Inventor 于淑娟刘冬梅李丹
Owner SOUTH CHINA UNIV OF TECH
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