Device and method for judging bacterium drug susceptibility by utilizing oxidation reaction of bacterium

A technology of oxidation reaction and sensitivity, applied in the direction of biochemical equipment and methods, electrochemical variables of materials, microorganism determination/inspection, etc.

Inactive Publication Date: 2010-10-06
解宇
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research model of microbial cell oxidation reaction using electr

Method used

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  • Device and method for judging bacterium drug susceptibility by utilizing oxidation reaction of bacterium
  • Device and method for judging bacterium drug susceptibility by utilizing oxidation reaction of bacterium
  • Device and method for judging bacterium drug susceptibility by utilizing oxidation reaction of bacterium

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Embodiment 1

[0031] The clinical diffusion method and dilution method susceptibility test results showed that Escherichia coli (K9512) strain was sensitive to common concentration antibiotic Ampicillin, and its minimum effective concentration was 5 μg / ml.

[0032] Take 3 parts each of 2ml of the Escherichia coli (K9512) solution, and the cells are centrifuged; the first part is placed in the antibiotic Ampicillin with a concentration of 50 μg / ml, mixed for 30 minutes, and the cells are centrifuged and washed. Finally, store the first Escherichia coli in 0.1M phosphate buffer (pH7.0); take the second Escherichia coli (K9512) liquid 2ml, and set the concentration of the bacteria to 5 μg after centrifugation Ampicillin / ml antibiotics, mixed reaction for 30min, and then the cells were centrifuged and washed, and the second part of Escherichia coli was stored in 0.1M phosphate buffer (pH7.0); the third part was taken Escherichia coli (K9512) liquid 2ml is not mixed with the antibiotic Ampicilli...

Embodiment 2

[0039] According to the results of the clinical diffusion method, take three parts of Escherichia coli (K9510) 2ml each that are sensitive to the antibiotic Erythromycin and moderately sensitive to the antibiotic Escherichia coli (K9510), and the bacterial cells are separated by centrifugation. , mixed reaction for 30min, after the cells were centrifuged and washed, the first part of Escherichia coli was stored in 0.1M phosphate buffer (pH7.0); the second part of Escherichia coli (K9510 ) liquid 2ml, after the bacterial cells were centrifuged, put in the antibiotic Ampicillin with a concentration of 50μg / ml, mixed for 30min, after the bacterial cells were centrifuged and washed, the second part of Escherichia coli was stored in 0.1M phosphate buffer (pH7.0). The third Escherichia coli (K9510) liquid 2ml was not mixed with antibiotics, and the bacterial cells were centrifuged and washed, and then stored directly in 0.1M phosphate buffer (pH7.0).

[0040] Take a few drops of th...

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Abstract

The invention relates to a device for judging bacterium drug susceptibility by utilizing oxidation reaction of bacterium, comprising a virtual voltage signal generator, a computer, a working electrode and a pair electrode; the bacterium subject to medicine treatment is adhered on the working electrode; wherein the voltage signal generator is connected with the computer and can apply voltage changing in preset voltage range between the working electrode and the pair electrode under the control of the computer; and meanwhile the computer samples the current flowing through the working electrode and the pair electrode, thus obtaining the volt-ampere map of the voltage and the current. In the device and method in the invention, bacterium subject to medicine treatment is placed on the surface of the working electrode, a scanning voltage is applied between the electrodes, when the scanning voltage reaches the oxidation level of the bacterium, auxiliary enzyme A in the bacterium joins the oxidation reaction to release electron, thus judging bacterium drug susceptibility according to the fact whether a peak valley is existed in the volt-ampere map.

Description

technical field [0001] The invention relates to a medical device and method for conducting drug sensitivity test on bacteria, specifically a device and method for judging the drug sensitivity of bacteria by utilizing the oxidation reaction of bacteria. Background technique [0002] Various pathogenic bacteria have different sensitivities to antimicrobial agents. It is of great significance to measure the susceptibility of bacteria to different drugs, corresponding to the choice of drugs in clinical treatment, so as to control infection in time and effectively. The current bacterial susceptibility test methods mainly include two types: diffusion method and dilution method. In the diffusion method, the paper method is commonly used, that is, the circular paper that has adsorbed the drug is covered on the surface of the bacterial culture medium, usually after 1 day of cultivation, the size of the inhibition zone is used to determine whether the bacteria are sensitive to the dr...

Claims

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Application Information

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IPC IPC(8): G01N27/48C12Q1/02
Inventor 解宇郑瑾
Owner 解宇
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