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Immunochromatography assay detection reagent and preparation method thereof

A technology for immunochromatography and detection reagents, which is used in measurement devices, analytical materials, instruments, etc., and can solve problems such as inability to correctly identify quality control lines, misjudgment, and inability of users to correctly judge analytes.

Inactive Publication Date: 2010-10-06
NANJING LIMING BIO PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for some users, they cannot correctly distinguish which is the quality control line and which is the test line, especially when there are multiple red lines at different positions on a test strip representing different analytes, users cannot Correctly judge the analyte corresponding to the red line, which may easily lead to misjudgment

Method used

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  • Immunochromatography assay detection reagent and preparation method thereof
  • Immunochromatography assay detection reagent and preparation method thereof
  • Immunochromatography assay detection reagent and preparation method thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0024] see image 3 , the preparation method of this immunochromatography reagent, comprises the following steps:

[0025] 1) Two kinds of antibodies, anti-biotin antibody and anti-early pregnancy antibody, are labeled with blue and red colored latex particles respectively:

[0026] 1.1). Label the anti-biotin antibody with blue latex particles: a, dilute the blue latex particles to a final concentration of 1% (mass percentage) with 0.01M PBS of pH7.4, then add anti-biotin antibody to make it final The concentration is 0.1mg / ml; b, put the above solution at 4°C to react overnight; c, add BSA with a final concentration of 1% to the solution obtained in step b and block for 1 hour; d, put the solution in step c The solution is centrifuged to obtain the precipitate of the latex antibody conjugate; e, the precipitate in step d is redissolved with 0.01M pH7.4 PBS containing 0.1% BSA, and the concentration after reconstitution is 1% (mass percentage) , and kept at 4°C for later us...

Embodiment 2

[0033] see Figure 4 , the preparation method of this immunochromatography reagent comprises the following steps

[0034] 1). Anti-biotin antibody, anti-rotavirus (ROTA) antibody and anti-adenovirus (ADENO) antibody are labeled with red, blue and green colored latex particles respectively:

[0035] 1.1). Label the anti-biotin antibody with red latex particles: a, dilute the red glue particles to a final concentration of 1% (mass percentage) with 0.01M PBS of pH7.4, then add the anti-biotin antibody to make the final concentration 0.1mg / ml; b, put the above solution at 4°C to react overnight; c, add BSA with a final concentration of 1% to the solution obtained in step b and block for 1 hour; d, put the solution in step c Centrifuge to obtain the precipitate of the latex antibody conjugate; e, the precipitate in step d is reconstituted with 0.01M PBS of pH7.4 containing 0.1% BSA, and the concentration after reconstitution is 1% (mass percentage), and Store at 4°C for later use...

Embodiment 3

[0046] see Figure 4 , the immunochromatography detection reagent comprises a PVC sheet 1, and the PVC sheet 1 is successively provided with a sample application area 2, a first reaction area 3, a second reaction area 4 and a water absorption area 5; the sample application area 2, the first The reaction zone 3, the second reaction zone 4 and the water absorption zone 5 are successively connected and arranged on the surface of the PVC sheet 1, the sample loading zone 2 is a glass fiber layer, the first reaction zone 3 is a polyester film, and the The second reaction zone 4 is a nitrocellulose membrane, the water-absorbing zone 5 is a water-absorbing filter paper, and the polyester film is provided with at least two latex-antibody conjugates, and the latex-antibody conjugates are marked with different colors; The nitrocellulose membrane is provided with multiple antibodies identical to the antibodies in the latex-antibody conjugate on the polyester membrane, and the multiple ant...

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Abstract

The invention discloses an immunochromatography assay reagent and a preparation method thereof. The immunochromatography assay reagent comprises a PVC sheet on which a sample adding zone, a first reaction zone, a second reaction zone and a water adsorption zone are successively arranged, wherein the sample adding zone is a glass fiber layer, the first reaction zone is a polyester membrane on which at least two emulsion antibody binding substances are arranged, the second reaction zone is a nitrocellulose membrane, the water adsorption zone is water adsorption filter paper, and the different emulsion antibody binding substances are marked with different colors; and the nitrocellulose membrane is provided with multiple antibodies the same as the antibodies in the emulsion antibody binding substances on the polyester membrane and an antigen, and the multiple antibodies and the antigen are respectively in a strip shape and are arranged on the nitrocellulose membrane in parallel. The preparation method of the immunochromatography assay reagent comprises the following four steps: utilizing color emulsions to mark the antibodies, carrying out film dispensing, carrying out emulsion dispensing and assembling. Therefore, the invention can simultaneously detect multiple substances, and can display the multiple substances through different colors.

Description

technical field [0001] The invention relates to a medical testing product and a production method thereof, in particular to an immunochromatography detection reagent and a production method thereof. Background technique [0002] Immunochromatography is a rapid diagnostic technique based on immune colloidal particle technology that emerged in the 1990s. Its principle is to fix specific antibodies on a certain zone of the nitrocellulose membrane, and when the dry nitrocellulose After one end of the film is immersed in the sample, the sample will move forward along the film due to capillary action. When moving to the area where the antibody is immobilized, the corresponding antigen in the sample will specifically bind to the antibody. If immunocolloid particles are used This area can be made to display a certain color, so as to realize specific immunodiagnosis. There are many types of test specimens: it can be used not only for blood test, but also for urine or saliva test, so...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/558
Inventor 刘光明
Owner NANJING LIMING BIO PROD CO LTD