Caragana korshinskii transcription factor ERF and gene thereof

A transcription factor and caragana technology, applied in genetic engineering, plant genetic improvement, angiosperms/flowering plants, etc., can solve problems such as unsatisfactory stress-resistant varieties and limited genetic resources related to stress resistance

Inactive Publication Date: 2010-10-20
INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently available stress-resistance-related gene resources are limited, and there are problems such as unsati

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1. Construction of a full-length cDNA library of twisted leaves:

[0010] Caragana korshinskii plants are cultivated in a culture bowl, cultivated in a greenhouse for 60 days, and subjected to drought treatment until the whole plant appears wilting. The young leaves are quickly frozen and ground in liquid nitrogen. The total RNA is extracted by TRIzole and dissolved in water. The mRNA is obtained by processing with the mRNA purification kit. Take mRNA for reverse transcription reaction, and then synthesize the second strand. After homogenization, connect with pDNR-LIB vector (see Creator for specific steps) TM SMART TM cDNA Library Construction Kit (Clontech Cat. No. 634903).

Embodiment 2

[0011] Example 2. Random EST sequencing of cDNA library:

[0012] Randomly select 3000 single clones from the library for 5'-end EST sequencing, then BLAST the sequenced sequences, and select ESTs similar to the verified drought-resistant genes.

Embodiment 3

[0013] Example 3 ESTs related to drought resistance by BLAST were selected, and their corresponding clones were tested to obtain the full-length DNA sequence of the candidate stress resistance gene.

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PUM

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Abstract

The invention discloses a drought correlative transcription factor ERF of caragana korshinskii and a gene thereof. The whole length of the gene is 1343bp, and comprises a 774bp opening reading frame; a coding product is drought induced ERF transcription factor composed of 286 amino acid residues, has an EREB/DNA binding region, can be combined with some drought reaction elements of a promoter region of a drought-related downstream gene, so as to regulate and control the transcription of a drought-enduring and cold-resistant correlative gene, and is a key element of a plant drought-enduring mechanism; the similarity of the amino acid sequence of the gene and an arabidopsis ERF gene is 47%; if the plant of the gene is constructed to express a carrier, and the drought enduring gene is introduced into all plants, thus the drought-enduring and cold-resistant performance can be improved.

Description

Technical field [0001] The present invention relates to a plant ERF transcription factor and its coding gene. Its function is to improve the stress resistance of plants and belongs to the technical field of plant bioengineering. Background technique [0002] Various abiotic stresses, including drought, salinity, low temperature and other abiotic stresses, are important factors restricting the production and development of crops, forests, grasses, and vegetables. my country’s arid and semi-arid arable land totals 570 million mu, accounting for 38% of the country’s arable land. my country’s annual grain output is reduced by 70-80 billion kilograms due to lack of water. Cultivating and popularizing stress-resistant plant varieties is the most effective way to increase their yield and quality, ensure national food security and improve the ecological environment. Although using plant's own disease resistance genes and some resistance-related genes, some progress has been made in resi...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N15/82C12N5/10A01H5/00
Inventor 郭九峰孙国琴白晨李晓东乔慧蕾
Owner INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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