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Method for increasing yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells

An extracellular polysaccharide, deep fermentation technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve problems such as no related literature reports on the yield of Nostoc polysaccharide, achieve high production efficiency, increase yield, Yield improvement effect

Inactive Publication Date: 2010-10-20
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the production of Nostocchia polysaccharides by submerged fermentation of Nostocchis cells mainly focuses on the optimization of fermentation conditions, such as the optimization of the medium and the optimization of the culture conditions; however, there is no relevant literature report on the use of controlled culture conditions to increase the yield of Nostocs polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: comparative example

[0015] The Nostocella cell species used in the present invention is isolated and preserved by Tianjin Key Laboratory of Industrial Microbiology (Chinese patent ZL031191101.0, inventors: Jia Shiru, Su Jianyu, Qiao Changsheng, date of authorization: March 8, 2006).

[0016] This invention separates and obtains the long-term preservation of Nostocchia cell species from the wild Nostocchis algae, carries out the suspension culture of Nostocys cells, adopts high-density culture technology, and adds the mixed nutrition mode of organic carbon source (Chinese patent 2006100113589.4, inventor: Jia Shiru Yu Haifeng, Su Jianyu, Lin Yongxian, published), using BG-11 medium supplemented with glucose, cultured for 7 days, the temperature was 24°C, the rotation speed was 140rpm, and the light intensity was 40-90μmol / m 2 ·s, the yield of polysaccharides of Nostocaria spp. was 2.25g / L.

Embodiment 2

[0018] The light reactor adopts the method of controlling light intensity and culture medium in stages, and the conditions such as temperature and rotation speed are the same as those in Comparative Example 1.

[0019] In the 4th day of the first stage of fermentation, nitrogenous BG-11 medium with 4g / L glucose was used, and the light intensity was controlled at 40~60μmol / m 2 s, on the fifth day of culture, feed nitrogen-free BG-11 medium with 2g / L sodium acetate and 3-3.5g / L glucose, adjust the pH above 9.0, and control the average light intensity of the liquid surface at 100-160μmol / m 2 ·s.

[0020] Using this step-by-step control strategy to produce polysaccharides from Nostoconia spp., the yield of polysaccharides obtained by ethanol precipitation was 3.5g / L.

Embodiment 3

[0022] The light reactor adopts the method of controlling light intensity and culture medium in stages, and the conditions such as temperature and rotation speed are the same as those in Comparative Example 1.

[0023] In the 4 days of the first stage of submerged fermentation, the aerobic BG-11 medium supplemented with 4g / L glucose was used, and the light intensity was controlled at 40-60μmol / m 2 s, harvest the cells on the 5th day of cultivation, all transfer to the nitrogen-free BG-11 medium that adds 2g / L sodium acetate and 4g / L glucose, adjust the pH of the medium above 9.0, and light intensity (average value of the liquid level) ) is controlled at 100~160μmol / m 2 ·s.

[0024] Using this step-by-step control strategy to produce the polysaccharides of Nostoconia spp., the yield of polysaccharides obtained by the two-stage fermentation broth was 4g / L by ethanol precipitation.

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Abstract

The invention belongs to the field of the submerged fermentation of microalgae cells, in particular to a method for increasing the yield of extracellular polysaccharide produced by the submerged fermentation of long thread moss cells. The method for increasing the yield of the extracellular polysaccharide of the long thread moss cells by controlling illumination intensity by stages and by a culture medium fermentation process has simple process and high production efficiency and is suitable for amplifying industrial production, and the polysaccharide of the long thread moss cells obtained by production can be used as a natural antiviral medicine or a food raw material after being separated and purified.

Description

(1) Technical field [0001] The invention belongs to the field of submerged fermentation of microalgae cells, and in particular relates to a method for increasing the yield of exopolysaccharide produced by submerged fermentation of Nostocchis cells. (2) Background technology [0002] Fa Cai is a terrestrial economic cyanobacteria that grows in the arid regions of Northwest and North my country, and has high nutritional value. Due to its harsh growth environment, the cells of Nostocia spp. secrete a large amount of colloidal substances to the outside of the cells during the growth process, wrapping the cells and algal filaments outside the body to form a colloidal sheath to protect the cells of Nostia sativa from drought, high temperature, and ultraviolet radiation. harm. The main components of colloids are polysaccharides, and these polysaccharides are of great biological significance for the resistance of the unfavorable growth environment of the Nostocs and the normal grow...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/89
Inventor 于海峰董永胜逄圣慧崔波宁维颖
Owner QILU UNIV OF TECH