Microcosmic optical field acquisition and three-dimensional reconstruction method and device

Abstract

The embodiment of the invention provides a microcosmic optical field acquisition and three-dimensional reconstruction method. The method comprises the following steps of: acquiring and recording optical field information of a microcosmic sample to acquire an image stack by controlling tiny movement of the microcosmic sample along the direction of an optical axis; building a statistical theory model according to the acquired image stack and a Poisson model corresponding to the image stack, and acquiring the gradient of the microcosmic sample according to a compressed sensing theory model; and building a joint model according to the statistical theory model and the compressed sensing theory model, and performing iterative solution on the joint model to acquire the three-dimensional structure of the microcosmic sample. Compared with the prior art, the method and a device provided by the embodiment of the invention have the advantages of higher universality, more remarkable effect and more flexible operation.

Description

technical field [0001] In order to achieve the above purpose, an aspect of the embodiment of the present invention discloses a method for collecting and three-dimensional reconstruction of micro-optic field, including the following steps: [0002] Collecting and recording the light field information of the microscopic sample to obtain an image stack by controlling the microscopic movement of the microscopic sample along the optical axis direction; Background technique [0003] Observing, analyzing and understanding the tissue structure of microscopic samples such as cells in three-dimensional form is a research hotspot of experts and scholars at home and abroad. Microscopic field collection and three-dimensional reconstruction can realize three-dimensional imaging and visualization of microscopic samples. [0004] At present, optical imaging systems that can realize three-dimensional imaging and visualization of microscopic samples mainly include stereomicroscopic imaging s...

AI Technical Summary

Problems solved by technology

[0005]The 3D-X-ray microscopic imaging system needs to use X-rays as the light source, which will cause great damage to the sample, and because the sample needs to be rotated on the workbench, several hundred to Thousands of images are used for 3D modeling, which generally takes about half an hour to an hour, plus the later 3D modeling time, the overall time-consuming is very long

[0009]2) The pinhole device makes the number of photons detected by the detector extremely small, so the image contrast is far inferior to that of the wide-field microscope imaging system

[0010]3) The use of the pinhole device determines that only one point of the sample can be focused and imaged at a time. Even if a more advanced pinhole device appears, multiple imaging at one point, the efficiency is also very low

[0011]4) The confocal microscopic imaging system uses laser light as the light source, which will cause photobleaching and photocleavage of the fluorescent probes carried in the sample, and seriously affect the viability of living samples

[0012]Aiming at the current situation that the existing 3D microscopic imaging system is difficult to take into account various requirements such as high image quality, no sample damage, high processing efficiency, low equipment cost and human comfort , it is of great significance to design and implement a 3D microscopic imaging system that can meet these requirements at the same time

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