The invention discloses an air-conditioner regulating method for keeping human comfort degree dynamically under a refrigeration mode. The air-conditioner regulating method includes the steps of firstly, presetting a mapping relation between the real-time ambient temperature (including real-time outdoor ambient temperature and real-time indoor ambient temperature) and the target comfort degree (including target indoor ambient temperature, target indoor humidity, target indoor wind velocity, target indoor oxygen content and target noise level); secondly, detecting the real-time ambient temperature, real-time indoor humidity, real-time indoor wind velocity, real-time indoor oxygen content and real-time indoor noise level, calculating to obtain the target comfort degree according to the preset mapping relation, and regulating the air-conditioner to reach the target comfort degree. After the air-conditioner is turned on, ambient temperature and current indoor comfort degree are detected in real time, the indoor comfort degree is then regulated to the target comfort degree, manual setting of temperature is avoided, and over-cool, over-heat, air-conditioning diseases and the like are also avoided.