Selenium-enriched yeast hydrolysate and production method thereof

A technology of selenium-enriched yeast and production method, which is applied in the directions of animal feed, animal feed, application, etc., can solve the problems of inability to achieve the effect of selenium supplementation, low absorption rate, animal harm, etc., and achieve the improvement of animal production performance and biological utilization. The effect of improving the degree and improving the color of the flesh

Active Publication Date: 2010-10-27
GUANGZHOU LEADER BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Selenium is an indispensable trace element in animal physiology. The existing sources of selenium supplementation in feed include inorganic compounds such as sodium selenite. These products have disadvantages such as low absorption rate and high toxicity. Therefore, adding inorganic seleni

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Strain preparation: Take 0.01ml of fresh beer yeast sludge, dilute it and spread it on a YPD plate; after culturing in a 30°C incubator for 24 hours, pick a single colony for microscopic examination; screen even and full beer yeast single colonies and transfer them to YPD test tubes After incubation at a constant temperature of 30°C for 24 hours on a slant, store in a refrigerator at 0°C for later use.

[0034] (2) Seed expansion and cultivation: the bacterial classification that step (1) makes is gradually expanded and cultivated in 1 ton of seed tanks with triangular flask and Karst jar;

[0035] (3) Fermentation and cultivation: the culture medium is put into 10 tons of fermentation tanks, and sterilized at 118° C. under high pressure; the seeds obtained in step (2) are inserted into the fermentation tanks by the differential pressure method for fermentation and cultivation; 2 tons of yeast sludge are produced The amount of medium required is as follows:

[0036...

Embodiment 2

[0051] (1) Strain preparation: Take 1ml of fresh beer yeast sludge, dilute it and spread it on the YPD plate; after culturing in a 20°C incubator for 48 hours, pick a single colony for microscopic examination; screen the uniform and plump single colony of beer yeast and transfer it to the inclined surface of the YPD test tube After culturing at a constant temperature of 20°C for 48 hours, they were stored in a refrigerator at 10°C for later use.

[0052] (2) Seed expansion and cultivation: the bacterial classification that step (1) is made is gradually expanded and cultivated in the 500L seed tank with a triangular flask and a Karl's jar;

[0053] (3) Fermentation culture: Put the culture medium into ten tons of fermentation tanks, and sterilize at 110°C under high pressure; the seeds obtained in step (2) are inserted into the fermentation tanks by the differential pressure method to carry out fermentation culture; produce 2 tons of yeast sludge The amount of medium required i...

Embodiment 3

[0068] (1) Strain preparation: take 0.05ml of fresh beer yeast sludge, dilute it and spread it on a YPD plate; after culturing in a 25°C incubator for 40 hours, pick a single colony for microscopic examination; screen even and full beer yeast single colonies and transfer them to YPD test tubes After culturing at a constant temperature of 25°C for 38 hours on a slant, store in a refrigerator at 5°C for later use.

[0069] (2) Seed expansion and cultivation: the bacterial classification that step (1) is made is gradually expanded and cultivated in the 500L seed tank with a triangular flask and a Karl's jar;

[0070] (3) Fermentation and cultivation: the culture medium is put into 10 tons of fermentation tanks, and sterilized at 110° C. under high pressure; the seeds obtained in step (2) are inserted into the fermentation tanks by the differential pressure method for fermentation and cultivation; 2 tons of yeast sludge are produced The amount of medium required is as follows:

...

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Abstract

The invention relates to a selenium-enriched yeast hydrolysate and a production method thereof. The selenium-enriched yeast hydrolysate product of the invention is obtained from the production technology of the invention, wherein every 1 kg of selenium-enriched yeast hydrolysate product contains 500-5000 mg of selenium. The production technology of the selenium-enriched yeast hydrolysate of the invention comprises the following steps of: (1) culturing beer yeast strains in a culture medium consisting of molasses, sulphuric acid, calcined soda, ammonium salt, phosphate, carbamide and sodium selenite raw materials; (2) centrifugal separating yeast culture solution, and obtaining selenium-enriched yeast cell; (3) adopting an autolysis method and an enzymatic method to break the wall of the selenium-enriched yeast cell; and (4) drying selenium-enriched yeast hydrolysate after wall breakage, and obtaining the selenium-enriched yeast hydrolysate product. The selenium-enriched yeast hydrolysate product produced by the invention can be applied on the feed field.

Description

technical field [0001] The invention relates to a feed additive, in particular to a selenium-enriched yeast hydrolyzate and a production method thereof. technical background [0002] Selenium is an indispensable trace element in animal physiology. The existing sources of selenium supplementation in feed include inorganic compounds such as sodium selenite. These products have disadvantages such as low absorption rate and high toxicity. Therefore, adding inorganic selenium can not only achieve the ideal The effect of supplementing selenium also pollutes the environment and is potentially harmful to animals; the other type is selenium-enriched yeast, which is difficult to digest because the yeast cell wall is relatively strong, so the absorption rate is not very high. Contents of the invention [0003] The object of the present invention is to provide a selenium-enriched yeast hydrolyzate that is easy to digest and absorb, and its organic selenium content reaches 500-5000 mg / ...

Claims

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Application Information

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IPC IPC(8): A23K1/16
Inventor 陶正国肖明红周应芳赵典惠
Owner GUANGZHOU LEADER BIO TECH
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