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Immune enhanced gene vaccine of hepatitis B virus core antigen and preparation method thereof

A core antigen, hepatitis B virus technology, applied in the field of genetic engineering, can solve the problems of peripheral immune tolerance, low immune response, insufficient activation of specific cellular immunity, etc., achieves low cost, simple preparation method, and promotes HBcAg-specific T cells. effect of immune response

Inactive Publication Date: 2010-11-17
ARMY MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, chronic HBV patients have problems such as low immune response, insufficient activation of specific cellular immunity, and peripheral immune tolerance. When designing therapeutic vaccines, it is necessary to enhance the ability of the immune system to recognize HBV to stimulate powerful specific cellular immunity.

Method used

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  • Immune enhanced gene vaccine of hepatitis B virus core antigen and preparation method thereof
  • Immune enhanced gene vaccine of hepatitis B virus core antigen and preparation method thereof
  • Immune enhanced gene vaccine of hepatitis B virus core antigen and preparation method thereof

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Experimental program
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Embodiment Construction

[0027] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0028] One, the preparation of the immunoenhanced gene vaccine of HBcAg

[0029] 1. Construction of recombinant eukaryotic vector for co-expression of HBcAg and OX40L genes

[0030] (1) Cloning of HBcAg full-length cDNA

[0031] According to the HBcAg gene sequence with GenBank accession number NC_003977.1, the following primers were designed and synthesized to amplify the full-length cDNA of HBcAg with restriction restriction sites at both ends: upstream primer F1: 5’-aat ctgcag ggcatggacatcg...

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Abstract

The invention relates to the technical field of gene engineering, in particular to an immune enhanced gene vaccine of a hepatitis B virus core antigen (HBcAg). The vaccine is an immune stimulating complex (ISCOM) vaccine consisting of HBcAg and OX40 ligand (OX40L) dual-gene co-expression recombinant eukaryotic vector, saponin Quil A, cholesterol and lecithin. The vaccine can promote immune response of specific T cells of the HBcAg and efficiently induce the anti-virus replication capacity of immune cells, and has good application prospect in the field of hepatitis B virus infection resistance. The invention also relates to a preparation method for the vaccine with simple and convenient operation and low cost.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to an immunoenhanced genetic vaccine of hepatitis B virus core antigen, and also relates to a preparation method of the vaccine. Background technique [0002] Hepatitis B virus (HBV) is a hepatotropic DNA virus. Hepatitis B is caused by HBV, mainly through blood, body fluids and mother-to-child transmission, and is an infectious disease with a chronic carrier state. At present, there are more than 300 million chronic HBV infections in the world, about half of which are in my country. This kind of infection is easy to develop into chronic hepatitis and cirrhosis, and a small number of cases can be transformed into primary hepatocellular carcinoma. It is currently the ninth cause of death among human diseases announced by WHO. Therefore, seeking an ideal anti-HBV drug has always been an urgent research topic. At present, chronic HBV infection is mainly treated with anti...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K39/29A61K39/39C12N15/79A61P1/16A61P31/20
Inventor 吴玉章杨曌
Owner ARMY MEDICAL UNIV
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