Method for enhancing high intensity focused ultrasound (HIFU) induced anti-tumor immune response

An anti-tumor immunity, focused ultrasound technology, applied in the medical field to improve the treatment effect and shorten the treatment time

Inactive Publication Date: 2010-12-01
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These studies can improve the therapeutic effect of HIFU on primary t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for enhancing high intensity focused ultrasound (HIFU) induced anti-tumor immune response
  • Method for enhancing high intensity focused ultrasound (HIFU) induced anti-tumor immune response
  • Method for enhancing high intensity focused ultrasound (HIFU) induced anti-tumor immune response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: HIFU treatment can attract DCs to accumulate in the surrounding areas of thermally denatured areas.

[0021] Attached figure 1 The shown HIFU tumor treatment device performs HIFU treatment on the MC-38 colon cancer tumor tissue inoculated subcutaneously on the right hind limb of the mouse. In the continuous wave mode (continuous wave, CW), the ultrasonic pressure is P + = 19.5 / P- = -7.2 (MPa), HIFU can rapidly increase the temperature of the focal area of ​​the tumor to 80°C in a short time of 4 seconds (attached figure 2 A), and produced a typical heat-denatured zone with clear margins in the treated area, measuring 1 × 5 mm (transverse × longitudinal direction) (attached figure 2 B). This is consistent with the thermal effect of tumor tissue produced by current clinical HIFU treatment. According to the results of intratumoral temperature measurement, the peripheral tissue temperature around the thermal injury area also increased, but the peak temperat...

Embodiment 2

[0022] Example 2: Tumor cells in the marginal region of thermal injury caused by HIFU have a strong immunostimulatory ability to activate DC.

[0023] Tumor cells cultured in vitro were treated with different HIFU for 4s, and their temperature was raised to 55°C and 80°C during the treatment time (simulating the heating of tumors around and within the focal region, respectively), and then compared with the cultured mouse bone marrow Derived DCs (BMDCs) were co-cultured, and markers of DC maturation (CD80 / CD86 expression levels and IL-12 secretion levels) were detected to detect whether tumor cells around the focal area and within the focal area had different abilities to stimulate DC maturation. attached Figure 4 The results shown indicated that 55°C-HIFU-treated tumor cells showed stronger immunostimulatory activity than 80°C-HIFU-treated tumor cells in inducing IL-12p70 secretion and stimulating the upregulation of surface CD80 and CD86 expression of DCs. It is shown that ...

Embodiment 3

[0024] Example 3: Sparse scanning strategy HIFU can produce similar therapeutic effects on primary tumors as dense scanning strategy, but the specific anti-tumor response induced by it is much higher than dense scanning strategy HIFU.

[0025] Using MC-38 mouse colon cancer and B16 melanoma tumor-bearing mice as animal models, two different HIFU scanning treatment strategies (ie, dense scanning and sparse scanning) were used for HIFU treatment of tumors. The dense scanning strategy adopts 1 mm interval of HIFU treatment at each point to generate densely arranged thermally denatured areas to simulate the current clinical treatment strategy; the sparse scanning adopts 2 mm interval of HIFU treatment at each point to preserve the surrounding tissue of each thermally denatured area. attached Figure 5 A shows densely arranged and spaced thermally denatured regions on MC-38 tumors caused by two scanning strategies. attached Figure 5 B and C show that although the sparse scanning...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the medical technology field, in particular relating to a method for enhancing high intensity focused ultrasound (HIFU) induced anti-tumor immune response. On the premise of having no change on the existing HIFU oncotherapy ultrasound strength, the method utilizes a sparse scanning strategy to enable tumor tissue heat denaturation spots generated by single HIFU treatment to have a proper spacing so as to reserve tissues around each heat denaturation area. The method can enable the tumor issues to have stronger capacity of recruiting and activating interdigitate cells and inducing out stronger anti-tumor immune response while having no influence on primary tumor thermal ablation curative effect by HIFU, thus killing epibiotic viable cells and preventing and curing tumour distant metastases. The method can improve overall tumor curative effect of HIFU and shorten curative time.

Description

technical field [0001] The invention belongs to the field of medical technology, and in particular relates to a method for enhancing the anti-tumor immune response level of a body when HIFU is used to treat tumors. Background technique [0002] As a new technology for tumor treatment, high-intensity focused ultrasound (HIFU) has been widely used in clinical practice. Its working principle is to use the characteristics of ultrasound penetration, directionality, and focus to focus low-energy ultrasound outside the body on the tumor tissue in the body, and high-energy ultrasound in the focal area can be absorbed in a short time (1 to 4 seconds) by the high thermal effect. ) to rapidly raise the temperature of the target tissue to above 65°C, cause irreversible coagulation necrosis of the target tissue, and kill tumor cells more accurately without damaging the surrounding tissue. Generally, the focus size of HIFU is 10 × 3 × 3 mm, so the treatment of the entire tumor needs to b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61N7/02
Inventor 胡振林刘芳丘磊丁力厉建中张俊平
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap