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Method for quickly detecting leucine content in rape leaves

A detection method, leucine technology, applied in the direction of measuring devices, scattering characteristics measurement, color/spectral characteristics measurement, etc., can solve the problems of laborious, complicated operation, high cost, etc., shorten the detection time, reduce environmental pollution, reduce The effect of testing costs

Inactive Publication Date: 2010-12-01
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a rapid detection method for leucine content in rapeseed leaves, which solves the problems of time-consuming, laborious, complicated operation, high cost, and environmental pollution in the existing detection method

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  • Method for quickly detecting leucine content in rape leaves
  • Method for quickly detecting leucine content in rape leaves
  • Method for quickly detecting leucine content in rape leaves

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Embodiment Construction

[0017] 248 rapeseed leaves were randomly collected. In order to make the collected rapeseed leaf samples more representative and diverse, the collected 248 leaves not only included rapeseed leaves under normal growth state (62 pieces in total), but also included sprayed rapeseed leaves. 186 pieces of rapeseed leaves with different concentrations (100, 500, 1000mg / L) of acetofen herbicide (62 pieces of rapeseed leaves were collected for each concentration gradient of 100, 500, 1000mg / L), and the spraying time was the five-leaf one-heart period. The method is foliar spraying. Propoxyfen herbicide is a kind of leucine inhibitor, which has inhibitory effect on leucine in rapeseed leaves, and can make the leucine content in rapeseed leaves have a wider range, so that the established model has better adaptability and robustness.

[0018] Put 248 rapeseed leaves in an oven at 105°C for 30 minutes, then dry them at 80°C to constant weight, then use a mortar to grind the dried rapesee...

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Abstract

The invention discloses a method for quickly detecting leucine content in rape leaves. The method comprises the following steps of: fixing green and drying the rape leaves and crushing the rape leaves to obtain leaf powder; acquiring the reflectivity of the rape leaves corresponding to 2,360nm optical wave, 2,386nm optical wave, 2,306nm optical wave, 2,314nm optical wave, 2,330nm optical wave, 1,194nm optical wave, 2,198nm optical wave, 1,420nm optical wave, 1,462nm optical wave, 2,106nm optical wave, 1,602nm optical wave, 1,878nm optical wave, 2,426nm optical wave, 1,814nm optical wave, 2,274nm optical wave, 1,676nm optical wave, 1,910nm optical wave and 2,052nm optical wave respectively; and substituting the reflectivity into a multiple linear regression respectively to calculate the leucine content in the rape leaves. In the method, multiple linear regression analysis is performed through minor characteristic wavelengths; and thus, the leucine content in the rape leaves is quickly and accurately detected, the detection time is greatly shortened, the environmental pollution is reduced, and the detection cost is reduced.

Description

technical field [0001] The invention relates to the field of rapeseed detection, in particular to a rapid detection method for leucine content in rapeseed leaves. Background technique [0002] Rapeseed is one of the main oil crops and nectar crops in my country. The planting area of ​​rapeseed accounts for more than 40% of the total area of ​​oil crops in the country, and its output accounts for more than 60% of the total output of edible vegetable oil in the country, ranking first in the world. The growth status of rapeseed in different periods is related to the yield and quality of rapeseed. The amino acid content in rapeseed leaves is a very important physiological index in the growth process of rapeseed, which is of great significance to physiological processes such as rapeseed leaf physiology and organic matter accumulation. Therefore, the growth status of rapeseed can be obtained by detecting the amino acid content in rapeseed leaves. In recent years, due to the redu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/49G01N21/35G01N21/3563
Inventor 何勇刘飞孔汶汶
Owner ZHEJIANG UNIV