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Method for establishing porphyria cutanea tarda (PCT) transgenic mouse model

A technology for transgenic mice and porphyria, applied in genetic engineering, plant genetic improvement, botany equipment and methods, etc., can solve the problems of heme oxidase-1, etc.

Active Publication Date: 2010-12-22
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research report on human or animal PCT induced by Heme Oxygenase-1 (HO-1)

Method used

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  • Method for establishing porphyria cutanea tarda (PCT) transgenic mouse model
  • Method for establishing porphyria cutanea tarda (PCT) transgenic mouse model
  • Method for establishing porphyria cutanea tarda (PCT) transgenic mouse model

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Embodiment

[0017] With reference to the accompanying drawings, the following embodiments are only used to illustrate the present invention without limiting the scope of the present invention.

[0018] 1. Obtain the target gene:

[0019] Primers were designed to amplify the HO-1 gene sequence in the mouse genome and cloned into pCAGG vector. HO-1 cDNA is initiated by chicken β-actin promoter and CMV enhancer, and HO-1 is connected with rabbit β-globin poly(A) termination signal ( figure 1 ). The plasmid was digested with SalI and DraI to obtain the linear transgene sequence, which was purified and recovered for microinjection.

[0020] 2. HO-1 was introduced into BDF-1 mouse fertilized eggs by microinjection and the production of positive mice

[0021] The linearized target gene fragment is injected into the pronucleus of BDF-1 mouse fertilized eggs to integrate with the mouse genome, and each cell will carry the fragment as the cells continue to divide. Three weeks after the pups wer...

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Abstract

The invention relates to the technical field of biology, in particular to a method for establishing a porphyria cutanea tarda (PCT) transgenic mouse model. The invention provides an animal model capable of stably inheriting and spontaneously generating a series of biochemical and pathologic characteristics conforming to the PCT. So far, the pathogeny and the pathogenesis of the PCT are not completely clear, and research reports about HO-1 participating in the PCT pathogenesis of human beings or animals are not seen. In the invention, a mouse model capable of spontaneously representing the PCT symptoms is established by overexpressing the HO-1 through a transgenic technology, thereby not only theoretically supplementing the pathogeny and the pathogenesis of the PCT, but also providing more valuable new ideas for the treatment of the PCT.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for establishing a transgenic mouse model of porphyria cutanea tarda. Background technique [0002] Porphyria cutanea tarda (PCT) is caused by molecular defects or reduced activity of the fifth enzyme in the biosynthesis of heme—uroporphyrinogen decarboxylase (URO-D) One of the most common porphyrias, it usually occurs after middle age, and it is more common in men. Research on PCT was first seen in 1950. In Turkey, people ate wheat seeds sprayed with the fungicide hexachlorobenzene, which triggered a PCT outbreak. Since then, people have used chlorinated hydrocarbons such as hexachlorobenzene to induce PCT in animals. Rodents can also induce PCT by ingesting iron, the precursor of heme synthesis, δ-amino-γ-levulinic acid, and alcohol. Another animal model used to study PCT is the URO-D knockout mouse. However, there is no research report on human or animal PCT induced b...

Claims

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Application Information

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IPC IPC(8): C12N15/63C12N15/53A01K67/027
Inventor 周凌云高旭马宁彭亚会邹朝霞惠洋
Owner HARBIN MEDICAL UNIVERSITY