Method for preparing direct seeding compression nutrition pot raw material
A nutrient bowl and raw material technology, which is applied in the preparation of organic fertilizers, organic fertilizers, and the treatment of biological organic parts, etc., can solve the problems of early growth of difficult seedlings, low labor intensity, and high labor intensity of strong seedlings, and achieve the goal of increasing the content Effect
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Embodiment 1
[0026] The preparation of embodiment 1 mold composite bacterial agent
[0027] 1) The preparation process of four single-strain preparations of Aspergillus niger, Aspergillus oryzae, Trichoderma viride and Geotrichum candidum
[0028] Aspergillus niger, Aspergillus oryzae, Trichoderma viride and Geotrichum candidum were inoculated into the Erlenmeyer flask with PDA liquid medium, cultivated at 30°C and 180rpm, so that the number of spores reached 2.5-6.0×10 8 Individual / mL promptly gets seed liquid, by 5-10% inoculum amount, seed liquid is inoculated to the substratum composition ratio and is: stalk 90%, bran 6%, (NH 4 ) 2 SO 4 2%, urea 1%, KH 2 PO 4 0.6%, MgSO 4 0.2%, CaCl 2 0.2%, in a solid-state fermentation medium with a moisture content of 60-70% of the total solid content, ferment at 30°C and turn over every 2-3 hours, and the fermentation product is vacuum-dried to moisture at 40°C ≤10% is a single-strain preparation. The spore content in the bacterial agent is...
Embodiment 2
[0031] The preparation of embodiment 2 yeast compound bacterial agents
[0032] 1) The preparation process of three single-strain preparations of Saccharomyces cerevisiae, Candida tropicalis and Candida utilis
[0033] In complete medium (glucose 2%, yeast extract 1%, peptone 2%, pH natural) solid slant medium, through twice activated Saccharomyces cerevisiae, Candida tropicalis, Candida utilis strains inoculated into the In the Erlenmeyer flask of liquid culture medium with complete medium, cultivate at 28°C and rotate at 180rpm, so that the number of cells reaches 7-9×10 8 Each / mL is the seed solution, inoculate the seed solution into the sterilized wort with a concentration of 8-15Bx according to the inoculum amount of 4-6%, at 28°C, the rotating speed is 180rpm, and the ventilation rate is 0.3-1v / v. Cultivate under the condition of min until the number of cells is 2.5-4.5×10 9 Individual / mL, that is, a single-strain preparation of three yeasts.
[0034] 2) Preparation o...
Embodiment 3
[0036] The preparation of embodiment 3 nitrogen-fixing bacteria composite bacteria agent
[0037] 1) The preparation process of two single-strain preparations of Alcaligenes faecalis and Azotobacter sphaeroides
[0038] In Asbby nitrogen-free medium (mannitol 10g, calcium carbonate 5g, potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.2g, sodium chloride 0.2g, calcium sulfate 0.1g, distilled water 1000mL, pH6.8-7.0) solid slant medium Inoculate Alcaligenes faecalis and Azotobacter sphaeroides that have been activated twice in the medium into the liquid medium Erlenmeyer flask equipped with Asbby nitrogen-free medium, and cultivate them at 32°C and 180rpm, so that the number of cells reaches 5-7× 10 8 seeds / mL to obtain the seed liquid, inoculate the seed liquid into Asbby nitrogen-free medium according to the inoculum amount of 6-8%, and cultivate until the cells are grown at 32°C, with a rotation speed of 180rpm and an air flow of 0.5-1v / v·min. Number 3-5.5×10 9 Eac...
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