Safety evaluation method for transgenic corns

Abstract

The invention discloses a safety evaluation method for transgenic corns. In the method, a holoprotein extract of the transgenic corns is acted on human lymphocytes through the subculture of a human lymphocytic series, and the safety of the transgenic corns is evaluated by detecting the damage degree of the human lymphocytes and the genetic damage degree. The evaluation method has the advantages of short period, low cost, simplicity, convenience, easy operation and high and accurate detection sensitivity, can be used for the safety evaluation of series models of the transgenic corns and relevant derived products thereof, and has the practical application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for evaluating the safety of transgenic corn. Background technique [0002] Whether genetically modified foods have genetic damage effects in the human body is the main reason why people doubt their safety. After the gene fragments modified by genetic engineering are introduced, the downstream gene transcription effect may be triggered, which may cause the final product to contain new components or change the content of existing components; the potential risk of regulating gene (promoter, terminator) transfer effects, such as transgene Soybean 35S promoter may be inserted into the upstream of an oncogene through horizontal gene transfer, activate and cause cancer. When multiple gene segments are introduced into an organism, the probability of unintended effects may be relatively high. The current technical research cannot fully grasp these unexpected unknown harmful factor...

AI Technical Summary

Problems solved by technology

[0003] At present, there are few studies on the safety evaluation of transgenic corn at home and abroad, mainly focusing on traditional toxicology methods (animal tests), using metabolite analysis, toxicokinetics, chronic toxicity, subchronic toxicity, reproduction tests and teratogenicity. In the process of safety evaluation, whole food feeding is mainly used to observe the impact on animal target organs.

However, the whole process is time-consuming and expensive. Due to the frequent fluctuations in the market price of corn, too long inspection time is obviously not of practical value, and the accuracy of the results is easily affected by many factors, such as: malnutrition caused by single feeding, and other factors in the food. Unknown inclusions, extrapolation of animal experiment results to human population differences, etc.

However, the detection level of traditional toxicology methods still stays at the level of pathological changes in tissues and organs, and does not involve the level of cells and genes

Images