Gene combination, primer and probe for detecting alcoholic liver disease susceptibility and application

Abstract

The invention discloses a gene combination, a primer and a probe for detecting alcoholic liver disease susceptibility and application. The gene combination comprises a combination of three genes closely related with an alcoholic liver disease, namely an ADH2 gene of alcohol dehydrogenase, an ALDH2 gene of acetaldehyde dehydrogenase and a CYP2E1 gene of cytochrome P4502. The gene combination comprises the following SNP sites: rs1229984 site of the ADH2 gene, rs671 site of the ALDH2 gene and rs2031920 site of the CYP2E1 gene. Whether the detected crowds carry 'alcoholic liver disease susceptible genes' are comprehensively detected and analyzed by detecting a group of genes and sites related with the alcoholic liver disease susceptibility, using the specific primer and the probe and combining a mononucleotide extending technique and a micro array chip technique so as to screen the alcoholic liver disease susceptible crowd from the crowds, change unhealthy lifestyles and fulfill the purpose of preventing.

Description

technical field [0001] The present invention relates to a gene combination, primer, probe and application, especially the gene combination, primer, probe and application for detecting alcoholic liver disease susceptibility. Background technique [0002] Alcoholic liver disease (ALD) is liver damage caused by long-term drinking, which can be manifested in three forms: alcoholic fatty liver, alcoholic hepatitis, and alcoholic cirrhosis. These three forms may exist alone or in admixture. According to statistics, there are about 15 million to 20 million people in the world who are alcoholics, and about 10% to 20% (1.5 million to 4 million) of them have alcoholic liver disease in varying degrees. In my country, with the continuous improvement of people's living standards and economic conditions, the incidence of ALD has also been increasing year by year in recent years. Alcohol has become the second leading cause of liver damage after viruses. [0003] After drinking, ethanol i...

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is, by detecting a group of genes and loci related to the susceptibility of alcoholic liver disease, using specific primers and probes, combining single nucleotide extension technology with microarray chip technology, to comprehensively detect and Analyze whether the tested population carries the "alcohol susceptibility gene", screen out the alcohol susceptibility population from the crowd, change bad living habits, and achieve the purpose of prevention