Gordonia alkanivorans and application in oil degradation
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A strain and alkane source technology, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as oil pollution, achieve good utilization ability, and broad application prospects of bioremediation
Inactive Publication Date: 2012-02-08
DALIAN UNIV OF TECH
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[0004] In order to solve the problem of environmental bioremediation of various oil pollution, especially the bioremediation of marine oil and diesel pollution, the present invention utilizes marine microbial resources to provide an alkane-derived Gordonia strain, which is effective for a variety of Hydrocarbons, aromatic compounds and liquid paraffin as the only carbon source growth and applications with degraded oils
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Embodiment 1
[0020] Implementation Example 1: The specific implementation examples of the present invention are decane, undecane, hexadecane, nonadecane, docosane, octacosane, Gordonia S104 can be 1.7% (v / v) of the above The substance grows as the only carbon source (Table 1), and can grow in a carbon-free medium containing aromatic compounds such as naphthalene, anthracene, phenanthrene, fluorene, and terphenyl (Table 2), and can be used to decompose a variety of hydrocarbon pollution things.
[0021] Table 1: The growth of alkane source Gordonia S104 in the medium with different alkanes as the sole carbon source *
[0022]
[0023] *Control is nutrient salt medium without any carbon. + means growth, ++ means good growth; +++ means vigorous growth, the same below, C10-C28 represents the number of carbon atoms of different alkanes
[0024] Table 2: The growth of alkane source Gordonia S104 in the medium with different aromatic compounds as the sole carbon source
[0025]
Embodiment 2
[0026] Implementation example 2: Gordonella S104 can utilize 10g / L diesel oil, soybean oil, liquid paraffin, olive oil to be the only carbon source growth, grow best in diesel oil ( image 3 ), and can grow in a medium containing contaminated crude oil from Xingang, Dalian ( Figure 4 ), can be used to degrade various oil pollution such as crude oil and diesel oil.
Embodiment 3
[0027] Implementation example 3: Gordonella S104 can grow well in seawater medium with 1% diesel oil without adding any inorganic salts, has floating characteristics, and can effectively promote the decomposition of oil pollution. Since the strain is isolated from sea mud samples, it can be effectively used in the treatment of marine diesel pollution.
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Abstract
The invention discloses a Gordonia alkanivorans and application in oil degradation, and belongs to the field of biotechnology. The Gordonia alkanivorans S104 is separated from a sea mud sample in Dalian area, and was collected in the Chinese General Microbial Culture Collection (CGMCC) on October 12th, 2010, wherein the collection number is 4224. The Gordonia alkanivorans is gram positive bacteria, has a rodlike shape, is determined by 16SrDNA sequence, and has high similarity (99.7 percent) with Gordonia alkanivorans, so the Gordonia alkanivorans is named Gordonia alkanivorans; however, the colonial morphology and the physiological and biochemical characters of the Gordonia alkanivorans are different from those of the model strains of the Gordonia alkanivorans. A plurality of hydrocarbons and aromatic compounds can be used by the Gordonia alkanivorans as sole carbon source, which comprises decane, undecane, hexadecane, nonadecane, docosane, octacosane, naphthalene, anthracene, phenanthrene, fluorene and terphenyl; the Gordonia alkanivorans can degrade oil, such as crude oil, diesel oil, soybean oil, olive oil and the like; and the Gordonia alkanivorans grows better in the diesel oil at 2 percent concentration, can degrade the crude oil, and has a promising application prospect in bioremediation of pollution of the crude oil and the diesel oil.
Description
technical field [0001] The invention relates to an alkane source Gordon's strain and its application in degrading oil, in particular to the application in bioremediation of petroleum and diesel pollution, and the application as a surfactant producing bacterium, which belongs to the field of biotechnology. Background technique [0002] With the continuous increase of social and economic development needs, the rapid development of oil extraction and processing industry, the environmental pollution caused by oil leakage in the process of oil extraction, transportation and use, and the discharge of oily waste is becoming more and more serious. With the increase of offshore oil exploration and transportation, offshore oil spills continue to occur, such as the offshore pollution caused by the Dalian oil pipeline explosion in July 2010, and the Gulf of Mexico pollution caused by the collapse of the BP offshore drilling platform in April 2010 etc., according to the US National Ocean...
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