Device for absorbing proteins from body fluids

A body fluid and protein technology, applied in the field of protein devices, can solve the problems that the diagnostic medium has not yet been carried out

Inactive Publication Date: 2011-02-16
丹通格诺斯蒂科斯有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0027] Another consequence of this is that the analysis of said samples is reserved for only a few universities, while the general use of GCF, for example, as a diagnostic medium has not yet been carried out or has not been possible.

Method used

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  • Device for absorbing proteins from body fluids
  • Device for absorbing proteins from body fluids
  • Device for absorbing proteins from body fluids

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0067] Example 1: Description of Hydrophilic Receiving Elements

[0068] The film is a uniform film that is integrally cast. The membrane can be sterilized by autoclaving at 135°C, 30 psi for 45 minutes. The integrity of the membrane can be determined by the bubble point test.

[0069] characteristic product design

[0070] Material The membrane is made of modified polyvinylidene fluoride

[0071] Measuring Rollstock width ≥ 280mm

[0072] Thickness 90μm≤x≤140μm

[0073] bubble point (H 2 O) 8hPa≤x≤13hPa

[0074] Flow time (H 2 O) x ≤ 20 seconds, 25°C, 1.9 bar (500ml through 47mm pan)

[0075] Pyrogenicity E.Coli Reference endotoxin (BVPP00000 only)

[0076] Shrinkage ≤2.2% in 45 minutes at 126°C, no value >2.7%

[0077] Wetability Filter paper is fully wetted in <30 seconds in 10 wt.% NaCl (Aq.)

[0078] Strength X≥771g

[0079] Elongation x≥15%

[0080] Extractability Oxidizable substances: After 100ml WFI flush (47mm), the effluent must be

[0081] ...

example 2

[0084] Example 2: Description of the device

[0085] Bottom element 70: Melinex 539 polyester film (175 [mu]m) produced by ICI.

[0086] Bonding Material 80: Medical Grade Adhesive

[0087] Support element 20:

[0088] Product number: ARCARE 7815

[0089] Adhesive: AS110, acrylic medical grade adhesive

[0090] Substrate: 51μm polyethylene film (PET)

[0091] Coating: Silicon PET coating

[0092] Identification element 40: Color film

example 3

[0093] Example 3: Sample Collection and Elution Method

[0094] GCF samples were collected according to standard methods by placing the GCF collection strip in the tooth pocket with forceps for 30 seconds. The GCF strips should be placed in the bag such that the identification element 40 faces its front and only 2-3mm of strips remain in the bag. After 30 seconds, the strips containing GCF were placed in 1.5ml eppendorf tubes.

[0095] Elution: Use a pipette to transfer the elution buffer (containing 15 mM Na 2 HPO 4 *12H 2 O, 7mM NaH 2 PO 4 *H 2 O, 550 nM NaCl, 0,05% 5-bromo-5-nitro-1,3-dioxane (BND), 0,2% bovine serum albumin (BSA) and 0,3% Tween 20 (Polysorbat 20) or Tetronic 1307 (BASFSE, block copolymer of ethylene oxide / propylene oxide)) was injected into the eppendorf tube containing the strip and incubated for 5 min at room temperature. Manually invert the tube at least 5 times, then gently remove the strip with tweezers. The sample (after elution) should be...

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PUM

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Abstract

A planar device for absorbing substances, especially proteins, such as enzymes, from body fluids, especially from gingival crevicular fluid or lacrimal fluid, comprising a receptor element (10), a support element (20), characterized in that - said receptor element (10) is hydrophilic and has a pore size of from 0.22[mu]m to 5[mu]m, especially from 0.5[mu]m to 3[mu]m, and consists of a plastic material or mixture of plastic materials, especially of an inert plastic material or a mixture of inert plastic materials; - said support element (20) is hydrophobic and covers one surface of the receptor element (10) at least partially, and - wherein the device further comprises a discriminator element (40) which is situated at the opposing surface of the receptor element (10).

Description

technical field [0001] The present invention relates to a device for absorbing proteins from bodily fluids, and to the use of said device. Background technique [0002] Gingival crevice fluid (GCF), saliva, tears or wound exudate are media that can be recovered in a non-invasive manner, thus requiring no surgical intervention, for example, at the dentist's office, at the pillow or during police control. Diagnostically, they play an important role because they may contain both human proteins and proteins from microorganisms, pharmaceuticals and their metabolites, stimulants (illicit drugs) and their metabolites, or free radicals. [0003] In general, the concentration of the substance in the fluid obtained in a non-invasive manner can be considered to be similar to the content of the substance in mammalian serum. Additionally, locally present substances, such as inflammatory markers, can reveal local pathological changes at the sampling site, such as periodontal inflammation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00A61B10/00
CPCH04L1/0027A61F2013/8473B01L3/5023H04L1/0026A61B10/0051B01L2300/0887
Inventor 库尔特·迈尔萨尔万·库马尔·穆尼亚尔
Owner 丹通格诺斯蒂科斯有限公司
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