Biochemical detection method and device for dentin phosphoprotein

A biochemical detection technology for dentin phosphoprotein, which is applied in the field of biochemical detection of dentin phosphoprotein, can solve the problems of no application and achieve high sensitivity, early diagnosis and simple operation
CN101949938BInactive Publication Date: 2013-05-22BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
Publication Date
2013-05-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a biochemical detection method and a biochemical detection device for dentin phosphoprotein, belongs to the technical field of electrochemical enzyme-linked immunoassay and relates to the early diagnosis of dental root resorption. In a device formed by connecting an electrolytic cell consisting of a three-dimensional micro-column array structure working electrode, a platinum auxiliary electrode and a calomel reference electrode, an external power supply and a detecting meter, the dentin phosphoprotein in the oral gingival crevicular fluid of a patient is extracted by afilter paper sucking method; after being purified, the dentin phosphoprotein serving as a sample to be tested is put into a centrifugal seal pipe; and the precise content of the dentin phosphoproteinis precisely measured by combining a double antibody sandwich method enzyme-linked immunological reaction and an electrochemical detection method. The method and the device can precisely detect the change rule of the dentin phosphoprotein in the gingival crevicular fluid and can realize early diagnosis of dental root resorption for the patient. The detection method has the advantages of simple operation, high sensitivity, no radiation damage and the like and has good application prospect in the clinical diagnosis of root resorption.
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Description

technical field

[0001] The invention belongs to the technical field of electrochemical enzyme-linked immunoassay. In particular, the invention relates to a biochemical detection method and device for dentin phosphoprotein for early diagnosis of tooth root resorption. Background technique

[0002] Most patients experience mild root resorption during the orthodontic process, and there will be no tooth loosening after the end of the orthodontic treatment. However, a small number of patients will experience severe root resorption, and the teeth will become loose or even fall out; orthodontic root resorption is the main complication of orthodontic treatment. If root resorption can be diagnosed early, it can prevent the patient's tooth from becoming loose or falling out. At present, imaging methods such as curved surface tomography, root tip radiograph, and CT are mainly used to diagnose tooth root resorption. It is urgent to find a more sensitive, safe and timely new method to ...

Claims

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