Biochemical detection method and device for dentin phosphoprotein

A biochemical detection technology for dentin phosphoprotein, which is applied in the field of biochemical detection of dentin phosphoprotein, can solve the problems of no application and achieve high sensitivity, early diagnosis and simple operation

Inactive Publication Date: 2013-05-22
BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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  • Abstract
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Problems solved by technology

However, it has not been applied in the field of dentin phosphoprotein biochemical detection

Method used

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  • Biochemical detection method and device for dentin phosphoprotein
  • Biochemical detection method and device for dentin phosphoprotein
  • Biochemical detection method and device for dentin phosphoprotein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0041] The method for determining the content of dentin phosphoprotein in the sample to be tested of the above-mentioned electrochemical detection system is:

[0042] Add a small amount of distilled water to a 10ml colorimetric tube, and add 1×10 -2 mol / L o-phenylenediamine solution 1ml, 0.1mol / L acetic acid-sodium acetate buffer solution with pH=4.8 2ml, 1×10 -3 mol / L hydrogen peroxide 4ml, dilute to 10ml standard scale, react at room temperature for 60 minutes. Take 3ml of this solution into another 10ml colorimetric tube, add 3ml of B-R buffer solution with pH=9, shake well and dilute to 10ml standard scale, pour 10ml of the final prepared solution into the aforementioned completed ELISA with In the polystyrene container of " double antibody sandwich structure ", react for 1 hour, insert working electrode 6, reference electrode 7 and auxiliary electrode 8 in the reaction solution (as shown in Figure 1), at -0.78V potential (compared to The reference electrode 7) measures ...

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Abstract

The invention discloses a biochemical detection method and a biochemical detection device for dentin phosphoprotein, belongs to the technical field of electrochemical enzyme-linked immunoassay and relates to the early diagnosis of dental root resorption. In a device formed by connecting an electrolytic cell consisting of a three-dimensional micro-column array structure working electrode, a platinum auxiliary electrode and a calomel reference electrode, an external power supply and a detecting meter, the dentin phosphoprotein in the oral gingival crevicular fluid of a patient is extracted by afilter paper sucking method; after being purified, the dentin phosphoprotein serving as a sample to be tested is put into a centrifugal seal pipe; and the precise content of the dentin phosphoproteinis precisely measured by combining a double antibody sandwich method enzyme-linked immunological reaction and an electrochemical detection method. The method and the device can precisely detect the change rule of the dentin phosphoprotein in the gingival crevicular fluid and can realize early diagnosis of dental root resorption for the patient. The detection method has the advantages of simple operation, high sensitivity, no radiation damage and the like and has good application prospect in the clinical diagnosis of root resorption.

Description

technical field [0001] The invention belongs to the technical field of electrochemical enzyme-linked immunoassay. In particular, the invention relates to a biochemical detection method and device for dentin phosphoprotein for early diagnosis of tooth root resorption. Background technique [0002] Most patients experience mild root resorption during the orthodontic process, and there will be no tooth loosening after the end of the orthodontic treatment. However, a small number of patients will experience severe root resorption, and the teeth will become loose or even fall out; orthodontic root resorption is the main complication of orthodontic treatment. If root resorption can be diagnosed early, it can prevent the patient's tooth from becoming loose or falling out. At present, imaging methods such as curved surface tomography, root tip radiograph, and CT are mainly used to diagnose tooth root resorption. It is urgent to find a more sensitive, safe and timely new method to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/545G01N27/27G01N27/28
Inventor 沙海亮白玉兴
Owner BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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