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Method for detecting quantity of nitrogen fixed by soybean root nodules

A soybean root nodule and detection method technology, which is applied in the direction of botany equipment and methods, measuring devices, seed and rhizome treatment, etc., can solve the problems of poor precision and achieve the effect of improving detection efficiency and accurate data

Inactive Publication Date: 2012-06-27
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides a method for detecting nitrogen fixation in soybean nodules in order to solve the problem of poor accuracy of existing detection methods for nitrogen fixation in soybean nodules

Method used

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specific Embodiment approach 1

[0006] Specific embodiment one: the detection method of the soybean nodule nitrogen fixation amount of the present embodiment, carry out according to the following steps: one, at first determine the soybean variety, select the non-nodulation soybean line that the male parent is similar and / or the female parent is similar, and then from the selected Select the non-nodulation soybean line that is closer to the plant physiological traits of the tested variety from the non-nodulation soybean lines, and then determine the gene sequence by molecular biology, and select the non-nodulation soybean line that is the closest to the tested variety as the control. Strain, obtain the homologous near allele non-nodulation soybean variety of tested soybean variety; The seeds of soybean varieties use 1.8% to 2.3% H 2 o 2 Soak in aqueous solution for 0.5h-1h, and then wash it with water; 3. The soil is sieved with a sieve with a 5mm aperture, and the fine soil after sieving is put into a clean...

specific Embodiment approach 2

[0008] Specific implementation mode two: the difference between this implementation mode and specific implementation mode one is: in step two, H 2 o 2 The mass concentration of the aqueous solution is 1.9%-2.2%, and the soaking time is 0.6h-0.9h. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0009] Specific implementation mode three: the difference between this implementation mode and specific implementation mode one is: in step two, H 2 o 2 The mass concentration of the aqueous solution is 2.0%, and the soaking time is 0.8h. Others are the same as in the first embodiment.

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Abstract

The invention relates to a method for detecting the quantity of nitrogen fixed by soybean root nodules, which relates to a method for detecting the quantity of the nitrogen fixed by root nodules and solves the problem of low accuracy of the conventional method for detecting the nitrogen fixed by the soybean root nodules. The method comprises the following steps of: determining the variety of detected soybeans and the variety of homologous near-allele non-nodulating soybeans; soaking seeds of the detected soybeans and the homologous near-allele non-nodulating soybeans in aqueous solution of hydrogen peroxide (H2O2) and then sowing the seeds in culture pots respectively, wherein three strains are planted in each pot and cultured under the same growth condition; performing destructive sampling on the strains at the maturation stage; detecting the total nitrogen quantity of the detected soybeans and the homologous near-allele non-nodulating soybeans respectively; and subtracting the totalnitrogen quantity of the homologous near-allele non-nodulating soybeans from that of the detected soybeans to obtain the quantity of the nitrogen fixed by root nodules of the detected soybeans. The method has the detection accuracy of over 90 percent and can be used for detecting the quantity of the nitrogen fixed by the root nodules of leguminous crops.

Description

technical field [0001] The invention relates to a method for detecting the amount of nitrogen fixation in root nodules. technical background [0002] Soybean root nodule is the organ of soybean nitrogen fixation, its main function is to fix atmospheric N 2 Converted into organic nitrogen, providing nitrogen nutrition for the animal. The nitrogen-fixing ability of soybean nodules determines its ability to fix nitrogen sources in the atmosphere to adapt to the environment, and also determines the growth of soybeans. As the core evaluation index of soybean nodule function, nitrogen fixation ability of soybean nodule has become an important aspect of soybean physiological research. There are two existing methods for detecting the amount of nitrogen fixation in soybean nodules, one is to use no nitrogen fertilizer as a control, and the other is to use isotope 15 The N-marked fertilizer is the test fertilizer, and the amount of nitrogen fixation in root nodules is calculated by...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01C1/00G01N33/00
Inventor 苗淑杰刘晓冰
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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