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Method for extracting alkaloid from Kratom

A technology of alkaloids and extracts, which is applied in the directions of drug combinations, pharmaceutical formulations, plant raw materials, etc., to achieve the effect of simple methods

Active Publication Date: 2011-05-11
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Kratom leaves can also be extracted by water and separated by resin to obtain Kratom crude extract, but there is no relevant report on extracting alkaloids from Kratom leaves with ethanol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) Take Kratom leaves, crush them to 10-20 meshes, extract with ethanol equivalent to 7 times (W / V) and 90% (v / v) of the weight of raw materials at room temperature for 20 hours, filter, and then use the residue equivalent to 7 times the weight of raw materials times (W / V), 95% (v / v) ethanol at room temperature, extract for 24 hours, filter, combine the extracts, concentrate to 0.5 times the weight of the raw materials, and obtain the concentrated solution for later use;

[0016] 2) adding absolute ethanol to the concentrated solution so that the concentration of ethanol therein is 90% (by mass), standing for 1 hour, centrifuging, collecting the supernatant, concentrating until there is no alcohol smell, and obtaining the concentrated supernatant;

[0017] 3) Gained concentrated supernatant was first diluted with water to 0.6 times the weight of the raw material, then extracted twice with ethyl acetate (the amount of ethyl acetate was 2 times the weight of the raw materi...

Embodiment 2

[0020] 1) Take Kratom leaves, crush them to 10-20 meshes, extract at room temperature with ethanol equivalent to 9 times (W / V) and 95% (v / v) of the weight of the raw materials for 24 hours, filter, and concentrate the extract to 20% of the weight of the raw materials 0.7 times, the resulting concentrated solution is for subsequent use;

[0021] 2) Add 95% (v / v) ethanol to the concentrated solution to make the ethanol concentration 90% (mass), leave it for 3 hours, centrifuge, collect the supernatant, concentrate until there is no alcohol smell, and obtain the concentrated supernatant liquid;

[0022] 3) The resulting concentrated supernatant is extracted 3 times with ethyl acetate (the amount of ethyl acetate is 3 times the weight of the raw material), the extracts are combined, and concentrated until there is no ethyl acetate in the extract, and the obtained extraction of ethyl acetate is removed. The solution was extracted twice with chloroform (the amount of chloroform was...

Embodiment 3

[0025] 1) Take Kratom leaves, crush them to 20-30 meshes, extract with ethanol equivalent to 8 times (W / V) and 95% (v / v) of the weight of raw materials at room temperature for 24 hours, filter, and then use the residue equivalent to 7 times the weight of raw materials times (w / v), 95% (v / v) ethanol at room temperature for 24 hours, filtered, and the residue was extracted with 7 times (w / v) of raw material weight (w / v), 95% (v / v) ethanol at room temperature for 24 hours hour, filter, combine extracts, concentrate to 0.8 times of raw material weight, and the gained concentrated solution is subsequent use;

[0026] 2) Add 95% (v / v) ethanol to the concentrated solution to make the ethanol concentration 90% (by mass), leave it for 2 hours, centrifuge, collect the supernatant, concentrate until there is no alcohol smell, and obtain the concentrated supernatant liquid;

[0027] 3) Gained concentrated supernatant was first diluted with water to 0.5 times the weight of the raw materia...

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Abstract

The invention discloses a method for extracting alkaloid from Kratom, which comprises the following steps of: 1) smashing Kratom leaves, extracting with 90 to 95 percent ethanol at normal temperature, combining extracts, and concentrating to obtain concentrate; 2) adding ethanol into the concentrate until the ethanol concentration is 90 (mass) percent, standing for 1 to 3 hours, centrifuging, collecting supernate, concentrating until alcohol is removed completely, and obtaining concentrated supernate; and 3) extracting the concentrated supernate with ethyl acetate for 1 to 3 times, re-extracting with chloroform for 1 to 2 times, concentrating the chloroform extract, removing the chloroform, freezing and drying to obtain Kratom alkaloid extract. The method is simple and easy to control, the total mass of the alkaloid in the obtained extract is more than 70 percent (assayed by High performance liquid chromatography (HPLC)), and the yield is 0.6 to 0.8 percent.

Description

technical field [0001] The invention relates to a method for extracting active ingredients in plants, in particular to a method for extracting alkaloids from Kratom. Background technique [0002] Kratom (Mitragyna specilsa) is a tree in the genus Mitragyna specilsa of the Rubiaceae family, native to Southeast Asia (Thailand, Malaysia, and Myanmar). Since ancient times, people in various countries of Southeast Asia have used the leaves of Kratom as herbal medicine. It is used as a stimulant (in low doses), a sedative (in high doses, it can cause hallucinations), a recreational drug, a pain reliever, a diarrhea medicine, and to treat opiate addiction. [0003] The active ingredients of Kratom are the alkaloids contained in it. At present, more than 25 alkaloids have been isolated and identified from kratom, among which the most abundant alkaloids include three indoles and two oxindoles, the three indoles are mitragynine , glamorous mitraphylline (paynanthine) and speciogyni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61P1/12A61P25/04A61P25/16A61P25/20A61P25/36A61K127/00
Inventor 宋云飞梁远盛赵军孙步祥汤凌志
Owner GUILIN NATURAL INGREDIENTS CORP
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