Corn oil rich in coenzyme Q10 and production method thereof
A production method and technology of corn oil, applied in the direction of edible oil/fat, food science, application, etc., to achieve the effect of preventing and treating cardiovascular diseases, maintaining vitality, and safe and reliable curative effect
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Embodiment 1
[0030] Preparation of coenzyme Q10: First culture Alcaligenes strains in nutrient agar medium, then inoculate them into fermenters, supplement the glucose fermentation liquid, and make the cell density reach 100 g / L of cell dry weight, at 15 ℃ for fermentation, the fermentation time is 15 days, and then the cells are collected by centrifugation to obtain the cell concentrate; 2% cellulase is added to the cell concentrate, and the temperature is kept at 50°C for 36 hours to dissolve the cells, and then add Ethanol, extract coenzyme Q10, and then use membrane separation method to remove impurities, and separate the mixed solution of ethanol and water; the above mixed solution of ethanol and water containing coenzyme Q10 is reduced at a temperature of 35°C and a vacuum of 80Pa. Pressure distillation to remove water and ethanol to obtain coenzyme Q10.
[0031] To prepare the corn oil of the present invention: first squeeze the corn germ to obtain crude oil, then process the crude ...
Embodiment 2
[0033] Prepared coenzyme Q10: first culture Alcaligenes strains in nutrient agar medium, then inoculate them into fermenters, supplement the glucose fermentation liquid, and make the cell density reach 110 g / L of cell dry weight, at 28 ℃ for fermentation, the fermentation time is 3 days, and then the cells are collected by centrifugation to obtain the cell concentrate; 1.5% cellulase is added to the cell concentrate, kept at 75°C for 12 hours, the cells are dissolved, and then added Ethanol, extract coenzyme Q10, and then use membrane separation method to remove impurities, and separate the mixed solution of ethanol and water; the above mixed solution of ethanol and water containing coenzyme Q10 is reduced at a temperature of 65°C and a vacuum of 20Pa. Pressure distillation to remove water and ethanol to obtain coenzyme Q10.
[0034] To prepare the corn oil of the present invention: first squeeze the corn germ to obtain crude oil, then process the crude oil through degumming, ...
Embodiment 3
[0036] Prepared coenzyme Q10: first culture Alcaligenes strains in nutrient agar medium, then inoculate them into fermenters, supplement the glucose fermentation liquid, and make the cell density reach 90 g / L of cell dry weight, at 25 ℃ for fermentation, the fermentation time is 13 days, and then the cells are collected by centrifugation to obtain the cell concentrate; 0.5% cellulase is added to the cell concentrate, kept at 60°C for 48 hours, the cells are dissolved, and then added Ethanol, extract coenzyme Q10, and then use membrane separation method to remove impurities, and separate the mixed solution of ethanol and water; the above mixed solution of ethanol and water containing coenzyme Q10 is reduced at a temperature of 50 ° C and a vacuum degree of 60 Pa. Pressure distillation to remove water and ethanol to obtain coenzyme Q10.
[0037] To prepare the corn oil of the present invention: first squeeze the corn germ to obtain crude oil, then process the crude oil through d...
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