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Pseudomonas stutzeri and application thereof to water body dephosphorization

A technology of Pseudomonas stutzeri and water body, applied in the field of Pseudomonas stutzeri and its phosphorus removal in water body, can solve the problems of few species of phosphorus accumulating bacteria and insufficient attention inside and outside, and achieve environmental adaptation Strong ability, prevention and control of water eutrophication, and the effect of saving production costs

Inactive Publication Date: 2012-11-14
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still very few isolated phosphorus-accumulating bacteria, and the method of using efficient bacteria to remove phosphorus is still in the exploratory stage, which is still far from meeting the needs of practical applications.
Moreover, the removal of low-concentration phosphorus (≤1.0mg / L) has not received enough attention at home and abroad, so qualitative and quantitative methods are used to screen high-efficiency phosphorus-accumulating strains for low-concentration phosphorus removal, and to explore its further application in phosphorus. The application in removal has important theoretical and practical significance

Method used

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  • Pseudomonas stutzeri and application thereof to water body dephosphorization
  • Pseudomonas stutzeri and application thereof to water body dephosphorization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, discovery of Pseudomonas stutzeri (Pseudomonas stutzeri) YG-26

[0026] 1. Isolation of strains

[0027] In September 2008, the water samples (surface sediment) of Taihu Lake in Wuxi City, Jiangsu Province were collected. On the way, the samples were placed in an ice box below 4°C, and after being brought back to the laboratory, 5g of the sample was weighed and placed in a triangle filled with 45mL of sterile water. Shake the bottle at 28°C and 180rpm for 1 hour. After the sample is evenly dispersed in sterile water, draw 0.5mL of the suspension into a test tube filled with 4.5mL of sterile water, mix well, and make a concentration of 10-1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 100 μL of each concentration of the suspension was drawn and spread on the YG solid medium plate, each sample was repeated three times, and cultured in a constant temperature incubator at 28°C for 3 days. Colonies with different shapes were streaked and purified, and labeled as YG-...

Embodiment 2

[0039] Embodiment 2, the phosphorus removal ability of immobilized Pseudomonas stutzeri YG-26 to natural wastewater

[0040] 1. Preparation of immobilized Pseudomonas stutzeri YG-26

[0041] Sodium alginate: purchased from Sinopharm Chemical Reagent Company, catalog number 30164428, molecular weight 222. Polyvinyl alcohol (PVA): purchased from Sinopharm Chemical Reagent Company, catalog number 30153083, with an average degree of polymerization (DP) of 2400-2500. Solution A (pH6.5): composed of water and solute; the solute and its concentration are: sodium alginate 1g / 100mL, polyvinyl alcohol 2g / 100mL. Solution B (pH6.5): composed of water and solute; the solute and its concentration are: CaCl24g / 100mL, boric acid5g / 100mL (volume percentage).

[0042] 1. Inoculate Pseudomonas stutzeri YG-26 into YG liquid medium, shake and culture at 180rpm at 28°C until logarithmic phase (OD 600 = 0.2).

[0043] 2. Take 20mL of bacterial liquid, centrifuge (6000rpm, 10min) to collect bacte...

Embodiment 3

[0055] Embodiment 3, the phosphorus removal ability of immobilized Pseudomonas stutzeri YG-26 to natural lake water

[0056] The natural lake water is taken from Taihu Lake, and the total phosphorus concentration is 0.2mg / L.

[0057] 1. Preparation of immobilized Pseudomonas stutzeri YG-26

[0058] Sodium alginate: purchased from Sinopharm Chemical Reagent Company, catalog number 30164428, molecular weight 222. Polyvinyl alcohol (PVA): purchased from Sinopharm Chemical Reagent Company, catalog number 30153083, with an average degree of polymerization (DP) of 2400-2500. Solution A (pH6.5): composed of water and solute; the solute and its concentration are: sodium alginate 1g / 100mL, polyvinyl alcohol 2g / 100mL. Solution B (pH6.5): composed of water and solute; the solute and its concentration are: CaCl24g / 100mL, boric acid5g / 100mL (volume percentage).

[0059] 1. Inoculate Pseudomonas stutzeri YG-26 into YG liquid medium, shake and culture at 180rpm at 28°C until logarithmic p...

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Abstract

The invention discloses a Pseudomonas stutzeri and application thereof to water body dephosphorization. The Pseudomonas stutzeri YG-26 provided by the invention has a preservation number of CGMCC No.4093. An immobilized bacterium can be obtained by using the YG-26 embedded by sodium alginate and polyvinyl alcohol. The strain YG-26 and the immobilized bacterium thereof can be used for maximumly removing phosphorus in sewage so as to effectively prevent and control the occurrence of water body nutrition enrichment. By using the strain or the immobilized bacterium provided by the invention to perform water body treatment, the removal ratio is high, the method is simple in operation, and the cost is low along with economical efficiency and environmental protection, so the strain or the immobilized bacterium can be widely applied to further treatment of the sewage, and have significant economic, environmental and social benefits.

Description

technical field [0001] The invention relates to a strain of Pseudomonas stutzeri and its application in phosphorus removal from water bodies. Background technique [0002] Eutrophication of water body has become one of the problems that seriously affect the quality of water body in the world. In my country, the area of ​​lakes with eutrophication has reached 5,000 square kilometers, and the area of ​​lakes with conditions for eutrophication has reached 14,000 square kilometers. Therefore, the control of eutrophication has become one of the technical difficulties that researchers at home and abroad focus on. [0003] A large number of studies have shown that phosphorus is the limiting factor for water eutrophication. In August 2008, based on 37 years of limnological experimental research, scientists from Canada and the United States jointly published an article in the Proceedings of the National Academy of Sciences (PNAS), proposing that the control of eutrophication in fre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 袁红莉李海峰杨金水李宝珍
Owner CHINA AGRI UNIV