Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing concentration-controllable broiler spleen transfer factor oral solution

A transfer factor and oral solution technology, applied in the field of oral products, can solve the problems of poor clinical effect, complicated preparation method and process, unstable product quality, etc., and achieve the effects of stable product performance, improved immunity and good clinical effect.

Inactive Publication Date: 2011-06-01
TIANJIN AGRICULTURE COLLEGE
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing broiler spleen transfer factor preparation method is complicated, the product quality is unstable, and the clinical effect is poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing concentration-controllable broiler spleen transfer factor oral solution
  • Method for preparing concentration-controllable broiler spleen transfer factor oral solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] See attached figure 1 .

[0025] The preparation method of the broiler spleen transfer factor oral solution with controllable concentration comprises washing the broiler spleen with physiological saline, and removing the fat and film with curved scissors. The spleen and distilled water were mixed according to the ratio of 1:1-1.5:1, and then pulverized with a high-speed tissue masher at 10,000 RPM for 5 minutes to ensure the fineness of the homogenate; put it into a 600mL plastic bottle, and put it in a -20°C refrigerator for at least 12hr. Dissolve quickly by rinsing with tap water and shaking several times. So repeated freezing and thawing more than 3 times. Put the frozen-thawed spleen tissue into a dialysis bag with a molecular cut-off of 10,000, seal it and put it into a white porcelain jar, and put the same volume of normal saline containing 0.2% formaldehyde solution in the container at the same time, and put it in a refrigerator at 4 °C Internal magnetic stir...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing concentration-controllable broiler spleen transfer factor oral solution, which belongs to the technical field of oral products. The method for preparing the concentration-controllable broiler spleen transfer factor oral solution comprises the following processes of: 1) cleaning, namely, cleaning the spleens of broilers and removing fat and capsules;2) crushing, namely, mixing the spleens with water according to the weight ratio of 0.5-3:1, and crushing the spleens by using a waring blender in a way of ensuring the fineness of homogenate; 3) repeated freeze thawing, namely, cooling spleen tissues at -15 to -30 DEG C for more than 10 hours, rapidly dissolving the cooled spleen tissues and repeating the freeze thawing for more than 3 times; and 4) dialysis, namely, placing the freeze thawing-treated spleen tissues into dialysis bags, sealing the dialysis bags, placing the sealed dialysis bags into a container filled with formaldehyde solution-containing normal saline, performing dialysis under magnetic stirring and performing dilution to obtain the clinically applied concentration-controllable broiler spleen transfer factor oral solution. The method has the advantages of simple process, economy, practicability, stable product performance, good clinical effects, wide application range and the like.

Description

technical field [0001] The invention belongs to the technical field of oral products, in particular to a method for preparing an oral solution of broiler spleen transfer factor with controllable concentration. Background technique [0002] At present, chicken transfer factor (Chicken Transfer Factor, referred to as CTF) is a kind of protein-free soluble and dialyzable protein composed of oligonucleotides and polypeptides, which is processed and prepared from broiler spleen as raw material. , Small molecular substances that can be ultrafiltered. The monitoring results of lymphocyte transfer test and serological test showed that: chicken transfer factor can significantly improve the level of cellular immunity (t>2.878, P<0.01), significantly improve the level of humoral immunity (t>2.101, P<0.05); CTF alone The injection has obvious therapeutic effects on Newcastle disease, bursal disease and Marek's disease, can reduce the mortality rate of affected chickens by 2...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/08A61P37/04A61K35/26
Inventor 李富桂张文生
Owner TIANJIN AGRICULTURE COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com