Method for separating and purifying L-alpha-glycerophosphorylcholine (L-alpha-GPC) by silica gel column chromatography

Abstract

The invention discloses a method for separating and purifying L-alpha-GPC by silica gel column chromatography, which belongs to the technical field of lipid development and application and comprises the following steps: removing Ca<2+> and Cl<-> from enzymatic hydrolysis reaction solution serving as a raw material by using ion exchange resin, converting a water phase into an alcohol phase, separating L-alpha-GPC from glycerol polyglycidyl ether (GPE), lysophosphatidylcholine (LPC) and other byproducts, decolorizing by active carbon, and dewatering through vacuum concentration to obtain a colorless and transparent product; directly passing alcoholysis reaction solution serving as a raw material through a silica gel column for separation, removing Na<+> by cation exchange resin, decolorizing by active carbon, and dewatering through vacuum concentration to obtain a product. According to the test of the indexes of the product, the chemical purity is 99.6 percent, the optical purity ee is 99 percent, and the melting point (mp) is 142 to 143 DEG C (wherein C is equal to 2.6, the H2O content is 16 percent, and the pH value is 5.8). The invention provides a new way of thought and a new method for separating and purifying L-alpha-GPC, and realizes the application of the silica gel column chromatographic separation and purification method in lipid science.

Description

A method for separating and purifying glycerophosphocholine by silica gel column chromatography technical field The invention discloses a method for separating and purifying glycerophosphorylcholine by silica gel column chromatography, and relates to the technical fields of medicinal chemistry and separation and purification. Background technique Glycerophosphatidylcholine (L-α-Glycerophosphorylcholine, L-α-GPC) is a naturally occurring water-soluble phospholipid metabolite in the body, as well as a choline source for the synthesis of acetylcholine and phosphatidylcholine. Its atomic structure is shown in Figure 1 Show. L-α-GPC has important medical application value. It can improve the cognitive ability of the brain, and even repair the partially damaged cognitive ability of patients with early senile dementia. It can protect liver tissue from toxic carbon tetrachloride and The fatty acid permeation produced by high-fat protein foods has the effects of anti-hyperlipidem...

AI Technical Summary

Problems solved by technology

The invention provides a method for separating and purifying L-α-GPC by silica gel chromatography column method, which solves the problems of cumbersome process, residual toxic ions, insufficient purity, insufficient optical rotation, and the existence of a large number of accompanying substances in the purification process.

The separation and purification method of silica gel column chromatography has the advantages of simple purification process, reusable eluent and silica gel, high purity of the product, and low cost. It is a convenient, economical, environmentally friendly, and commercializable method. Large-scale purification and separation methods, there are no reports on this aspect at home and abroad

Images