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Method for detecting single nucleotide polymorphism of cattle SH2B1 gene

A single nucleotide polymorphism, gene technology, applied in the field of molecular genetics

Inactive Publication Date: 2012-10-31
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At home and abroad (except SH2B1 and human obesity) there is no research on the genetic variation of SH2B1 gene in animals

Method used

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  • Method for detecting single nucleotide polymorphism of cattle SH2B1 gene
  • Method for detecting single nucleotide polymorphism of cattle SH2B1 gene
  • Method for detecting single nucleotide polymorphism of cattle SH2B1 gene

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Embodiment Construction

[0026] The invention detects the single nucleotide polymorphism that may result in a change in the conformation of the encoded protein due to the missense mutation at the 2795th position of the SH2B1 gene of yellow cattle, so as to be used for marker-assisted selection of growth traits of Chinese yellow beef and to quickly establish genetic resources Excellent cattle population. In the following, the present invention will be further described in detail in combination with the detection of specific samples and the association of traits, which is an explanation of the present invention rather than a limitation.

[0027] a. Design of PCR-RFLP primers for the first exon region of cattle SH2B1 gene

[0028] Taking the bovine (NW_001494303) sequence published by NCBI as a reference, Primer 5.0 was used to design PCR primers capable of amplifying the SNP site in the first exon region of the cattle SH2B1 gene. The primer sequences are as follows:

[0029] Upstream primer: cagcaactcc...

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Abstract

The invention discloses a method for detecting single nucleotide polymorphism of a cattle SH2B1 gene, which comprises the following steps: by using the whole genome DNA (deoxyribonucleic acid) of cattle to be detected, which contains an SH2B1 gene, as the template and using a primer pair P as the primers, carrying out PCR (polymerase chain reaction) amplification on the cattle SH2B1 gene; digesting the PCR amplification product by using a restriction endonuclease BglI, and carrying out agarose gel electrophoresis on the amplified segment which is subjected to enzyme digestion; and identifyingthe single nucleotide polymorphism of the 2795th site of the cattle SH2B1 gene according to the result of the agarose gel electrophoresis. Since the functions of the SH2B1 gene are related to four important growth traits, including body weight, daily weight gain, body oblique length and chest circumference, the method disclosed by the invention lays foundation for the establishment of the relationship between the SNP (single mucleotide polymorphism) of the SREBPlc gene and the growth traits, thereby facilitating the marker-assisted selection of growth traits for Chinese cattle beef and quickly establishing cattle populations with excellent genetic resources.

Description

technical field [0001] The invention belongs to the field of molecular genetics and relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a method for detecting the 2795th single nucleotide polymorphism of cattle SH2B1 gene. Background technique [0002] Gene polymorphism refers to the difference in genome sequence between different species or different individuals within the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, mainly including base substitutions, insertions, deletions and duplications. Changes in sequence copy number. [0003] Single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP) is a type of genetic marker system proposed by Lander (1996), a scholar at the Human Genome Research Center of the Massachusetts Institute of Technology. Polymorphism caused by acid (A / T / C / G) substitution. Its variant forms include: transversion, conversion, insertion and deletion, etc., w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 陈宏杨明娟屈炼刘俊霞蓝贤勇雷初朝
Owner NORTHWEST A & F UNIV