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Kit for determining sex of sheep early embryo

A kit and embryo technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of limited application and reduced embryo activity, and achieve the effect of improving the success rate and shortening the detection time.

Inactive Publication Date: 2013-01-23
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of PCR sex identification requires the use of large and medium-sized equipment such as PCR instruments, high-speed centrifuges, electrophoresis instruments, and gel imaging systems during the amplification and detection of PCR products, and the entire detection time exceeds 3 hours, which reduces the activity of embryos and limits its application in clinical gender identification

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  • Kit for determining sex of sheep early embryo
  • Kit for determining sex of sheep early embryo
  • Kit for determining sex of sheep early embryo

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Embodiment Construction

[0021] The following are embodiments of the present invention:

[0022] 1) The two specific primers SRY gene primers and the internal standard Callipyge gene primers were synthesized by Dalian Bao Biological Company, of which:

[0023] SRY upstream primer 5′-ACGCCTTCATTGTGTGGTCTCG-3′,

[0024] SRY upstream primer 5'BIOTIN-CCTTTTCCACTCGTATCCCCAGC-3';

[0025] Callipyge upstream primer: 5′-CGTGTCCTGGTCTATTTTCGG-3′,

[0026] Callipyge downstream primer: 5'BIOTIN--ATCACCCACACCCTGCCTT-3';

[0027] 2) The two probes, SRY probe and Callipyge probe, were synthesized by Dalian Bao Biology Co., Ltd., of which:

[0028] The SRY probe is '-GGTGGCTCTAGAGAATCCCAAATTGCAAAACTC-3'-DIG,

[0029] The Callipyge probe is 5′-GGATCTGACAGGTGGTCCCAGCCCTCGGGCCCC3′-FITC;

[0030] 3) Preparation of gold standard test paper

[0031] The end-to-end connection of the PVC liner material is fixed sequentially on the sample-loading water-absorbing layer made of porous fiber material, the reaction pad mad...

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Abstract

The invention discloses a kit for determining the sex of a sheep early embryo. The kit disclosed by the invention is provided with two pairs of specific primers, two probes and colloidal gold test strips, wherein the two pairs of specific primers are respectively SRY gene primers and interior label Callipyge gene primers, and the two probes are respectively an SRY probe and a Callipyge probe; each colloidal gold test strip is composed of a conjugate pad and a reaction pad; the conjugate pad is formed by coating colloidal gold on a BIOTIN antibody; the reaction pad is connected with the tail end of the colloidal gold conjugate pad, composed of a nitrocellulose membrane and respectively coated with a detection area and a quality control area; and the detection area is coated with an FITC (fluoresceinisothiocyanate) antibody, and the quality control area is positioned downstream from the detection area and coated with a DIG (digoxigenin) antibody.

Description

technical field [0001] The invention relates to a detection kit and a method for using the detection kit. Specifically, the invention is a kit for identifying the sex of early sheep embryos. Background technique [0002] Animal sex control is a bioengineering technology that can significantly increase the reproductive rate of animals. It is of great significance to animal breeding, production and the prevention and treatment of genetic diseases. Sex determination of early embryos is the main route of sex control. By controlling the sex ratio of offspring, the maximum economic benefits of sex-restricted and sex-affected production traits can be brought into full play. For example, the production of some economic traits such as meat, eggs, milk, hair, and velvet all requires animals of a specific sex for production. Controlling the sex ratio of offspring can also increase the intensity of selection, speed up the breeding process, and promote the development of efficient anim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 岳耀敬杨博辉郎侠牛春娥刘建斌冯瑞林孙晓萍郭建郭婷婷
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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