Kit and method for detecting melamine by virtue of fast visual colorimetry

Abstract

The invention discloses a kit and method for detecting melamine by virtue of fast visual colorimetry. The kit comprises: A, nanogold solution with particle size of 5-10nm; B, 100-300g / L trichloroacetic acid aqueous solution; C, 3M NaOH aqueous solution; D, 2Ml and 5mL centrifuge tubes; E, 0.22mum filter; F, plastic suction pipe; and G, a standard melamine colorimetric card. In the kit disclosed by the invention, the structure is simple, the cost is low and the sensitivity is high; the detecting method disclosed by the invention is strong in interference resistance for sample matrix effect; for simplified pretreatment, not only is the efficiency improved, and the detection period is shortened, but also a centrifugal machine and special technicians are not needed, and the implementation is easy; and without using any instrument such as a spectrometer, whether the melamine is contained as well as the content of the melamine can be directly judged by color comparison with naked eyes, so that the detection cost is low, the carry is convenient and the detection time is short.

Description

Kit and detection method for rapid visual colorimetric detection of melamine technical field The invention relates to a method for visually detecting melamine based on unmodified nano-gold, in particular to a kit and a detection method for rapid visual colorimetric detection of melamine. Background technique Since a series of incidents caused by melamine in 2008, fatal flaws in dairy product inspection technology have been exposed, leading to a crisis of integrity in my country's dairy industry and making my country's dairy product safety inspections face huge challenges. Melamine is a triazine nitrogen-containing heterocyclic organic chemical raw material. It is often used to manufacture melamine-formaldehyde resin. It is a commonly used fireproof material in the construction industry and is widely used in plastics, decoration, coatings and papermaking industries. The third volume of the "International Chemical Safety Handbook" jointly compiled by the International Progr...

AI Technical Summary

Problems solved by technology

At present, the commonly used methods for the detection of melamine in raw milk and milk products at home and abroad include the US FDA method (GC-MS and HPLC) and the high performance liquid chromatography (HPLC) in the national standard method (GB / T22388-2008). -mass spectrometry / mass spectrometry (HPLC-MS / MS), gas chromatography-mass spectrometry (GC-MS), although these methods can realize the accurate detection of melamine, but it requires sophisticated instruments, complicated operations, high detection costs; in addition Including surface-enhanced Raman spectroscopy (SERS), enzyme-linked immunosorbent assay (ELISA), etc. Although relatively fast and simple, the detection time is long and the cost is high

Moreover, the above methods all require professional technicians to operate and the pre-processing is complicated

Some detection methods are difficult to popularize among most food manufacturers and ordinary consumers

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