Inverted digital holographic microscope

A digital holographic and inverted technology, applied in the field of digital holography, can solve the problems of inability to observe living cells in a petri dish with an upright structure, and the large size and weight of a digital holographic microscope, achieving high integration, few optical components, and small size. Effect

Inactive Publication Date: 2011-07-13
BEIJING UNIV OF TECH
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Problems solved by technology

[0003] In order to solve the technical problem that the digital holographic microscope has a large volume and weight, and adopts an upright struc

Method used

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Embodiment Construction

[0017] Below in conjunction with accompanying drawing and specific embodiment the present invention will be further described:

[0018] The layout of this embodiment is as follows figure 1 Shown:

[0019] A fiber coupler 4 is placed in front of the laser 5, and the fiber coupler 4 is connected to the fiber beam splitter 3 through an optical fiber, and the fiber beam splitter 3 connects two optical fibers connected to the fiber collimator 1 and the fiber collimator 6, respectively. A sample stage 10 for holding a sample 11 is placed under the fiber collimator 1, and a microscopic objective lens 9 is placed under the sample stage 10. side. A CCD camera 7 is placed below the beam combining crystal 8 .

[0020] The light emitted by the laser 5 is coupled into the optical fiber through the optical fiber coupler 4, and is divided into two paths by the optical fiber beam splitter 3: the first path is the object light, and the divergent spherical light wave emitted by the optical f...

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Abstract

The invention discloses an inverted digital holographic microscope, which belongs to the digital holographic technical field, and can be used for three-dimensional real-time appearance measurement and biological cell imaging. In the inverted digital holographic microscope, an optical fiber coupler 4 is arranged in front of a laser 5 and connected with an optical fiber beam splitter 3 through an optical fiber; the optical fiber beam splitter 3 is connected with two paths of optical fibers respectively connected with an optical fiber collimator 1 and an optical fiber collimator 6, a sample stage 11 for placing a sample 13 is arranged under the optical fiber collimator 1, and the sample stage 11 is connected with a two-dimensional translation stage 12; a micro objective mounted on a one-dimensional translation stage 10 is located under the sample stage 11, and the micro objective 9 and the optical fiber collimator 6 are aligned with two mutually vertical sides of a beam combination crystal 8; and a CCD (charge-coupled device) camera 7 is located under the beam combination crystal 8. The inverted digital holographic microscope can be used for observing the living cell which is located at the bottom of a culture dish and grows along a wall of the culture dish with a high resolution ratio for long time, and is highly integrated and small in volume; moreover, with the optical fiber connection, the laser can be freely mounted on other parts of the system.

Description

technical field [0001] The invention discloses an inverted digital holographic microscope, which belongs to the technical field of digital holography and can be used for three-dimensional real-time shape measurement and biological cell imaging. Background technique [0002] In recent years, the development of biomedicine has promoted the development of observation technology at the scale of biological cells. Traditional optical microscopes cannot measure the three-dimensional morphology of biological cells; while confocal microscopes have high resolution, but due to the need to calibrate biological samples, it will affect them, which is not conducive to the need for non-destructive observation. As a microscopic imaging technology, digital holography is non-destructive, real-time, and can obtain quantitative phase distribution, which is exactly its advantage in imaging biological samples. Living biological cells are generally transparent structures, so their phase images can...

Claims

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Application Information

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IPC IPC(8): G02B21/00G02B21/36G03H1/08G01B9/04G01N21/84
Inventor 张亦卓王大勇王云新江竹青万玉红
Owner BEIJING UNIV OF TECH
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