Method for extracting and separating pueraria flavonid and pueraria polysaccharide
A technology of Pueraria flavonoids and Pueraria polysaccharides, applied in the direction of organic chemistry, can solve the problems of difficult separation of Pueraria flavonoids and Pueraria polysaccharides, large amount of ethanol, and many impurities, and achieve the effects of convenient operation, increased content, and simple process
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0020] 20 g of Pueraria lobata produced in Yunnan, extracted Pueraria flavonoids with 75% ethanol solution (mass ratio), according to the solid-to-liquid ratio of Pueraria lobata (g) and ethanol solution (mL) 1:20, in a 1 L flask, reflux extraction at 80°C 4 h, filter to obtain ethanol extract A and primary filter residue, and then use 75% ethanol solution (mass ratio) to reflux extraction at 80°C for 4 h at a solid-to-liquid ratio of 1:20 for the primary filter residue, and obtain ethanol extract B and the secondary filter residue, and combine it with ethanol extract A to obtain 750 mL of pueraria flavonoids extract; extract the pueraria polysaccharide from the secondary filter residue with water, according to the solid-liquid ratio of filter residue (g) to water (mL) 1:20, 100°C Extract under reflux for 4 h, filter to obtain 380 mL of water extract, and discard the filter residue; concentrate the water extract to obtain 10 mL of water extract concentrate and recover 368 mL of...
Embodiment 2
[0022] 20 g of Pueraria lobata produced in Hubei, extracted Pueraria flavonoids with 80% ethanol solution (mass ratio), according to the solid-liquid ratio of Pueraria lobata (g) and ethanol solution (mL) 1:5, in a 500 mL flask, reflux extraction at 80 °C 1 h, filter to obtain ethanol extract A and primary filter residue, and then use 80% ethanol solution (mass ratio) to reflux extraction at 80°C for 4 h at a solid-liquid ratio of 1:5 for the primary filter residue, and filter to obtain ethanol extract B and the secondary filter residue, the ethanol extract B and the ethanol extract A were combined to obtain 190 mL of Pueraria flavonoids extract; the secondary filter residue was extracted with water, and the solid-liquid ratio of the filter residue (g) to water (mL) was 1:5. Reflux extraction at 100°C for 4 h, filter to obtain 90 mL of water extract, and discard the filter residue; concentrate the water extract to obtain 3 mL of water extract concentrate and recover 86 mL of wa...
Embodiment 3
[0024] 20 g of Pueraria lobata produced in Zhejiang, extracted Pueraria flavonoids with 75% ethanol solution (mass ratio), according to the solid-to-liquid ratio of Pueraria lobata (g) and ethanol solution (mL) 1:10, in a 500 mL flask, reflux extraction at 80 °C 2 h, filter to obtain the ethanol extract A and the primary filter residue, the primary filter residue is then extracted with 75% ethanol solution (mass ratio) according to the solid-to-liquid ratio of 1:20 at 80°C for 2 h, and filtered to obtain the ethanol extract B and secondary filter residue, ethanol extract B and ethanol extract A were combined to obtain 380 mL of kudzu root flavonoids extract; the filter residue after the second extraction of ethanol solution was extracted with water to extract kudzu root polysaccharides, and solidified according to the filter residue (g) and water (mL) Liquid ratio 1:10, reflux extraction at 80°C for 2 h, filter to obtain 190 mL of water extract, discard the filter residue; conc...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap