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Method for detecting trace lipid-soluble tea polyphenol in vegetable oil

A technology of fat-soluble tea polyphenol and detection method, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by chemical reaction of the material, etc., can solve the problem that there are not many fat-soluble tea polyphenols and no reports and other problems, to achieve the effect of convenient supervision and management, easy operation and good application prospects

Active Publication Date: 2011-07-20
SHANDONG LUHUA GROUP
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  • Application Information

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Problems solved by technology

[0004] The research on water-soluble tea polyphenols has been very extensive, and there are not many reports on fat-soluble tea polyphenols. The detection of its higher content can refer to the method of water-soluble tea polyphenols, but adding a small amount of fat-soluble tea polyphenols to vegetable oils The detection technology has not been reported at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1, a method for detecting trace fat-soluble tea polyphenols in vegetable oil is:

[0034] Make the standard curve of ethyl gallate and analyze the content of the active ingredients of fat-soluble tea polyphenols: prepare more than 6 standard solutions of ethyl gallate with a concentration of 0.01-0.05 mg / mL. In this example, 0.015 mg / mL is selected. , 0.02mg / mL, 0.025mg / mL, 0.03mg / mL, 0.035mg / mL, 0.04mg / mL, 0.045mg / mL ethyl gallate standard solution, using ferrous tartrate colorimetric method, under 540nm wavelength conditions Measure the absorbance A1 with a spectrophotometer, take the concentration of ethyl gallate x as the abscissa, and the absorbance y as the ordinate, draw the standard working curve as y=14.248x, R 2 =0.9994; accurately weigh 0.2g of fat-soluble tea polyphenols, add 2-15mL of absolute ethanol, dissolve with ultrasonic aid, dilute to a 25mL volumetric flask with water, take 1mL and dilute with water into a 50mL volumetric flask to be tested, use...

Embodiment 2

[0037] Example 2, a method for detecting trace amounts of fat-soluble tea polyphenols in vegetable oils, making a standard curve of ethyl gallate and the method for analyzing the content of active ingredients of fat-soluble tea polyphenols are the same as in Example 1, making fat-soluble tea polyphenols The standard addition curve is:

[0038] Add fat-soluble tea polyphenols to pure peanut oil to prepare 2-15g of more than 6 oil samples containing 0-400ppm fat-soluble tea polyphenols, of which one blank sample of vegetable oil must be included, that is, 0ppm fat-soluble tea polyphenols In this embodiment, the concentrations of the other seven oil samples are 100ppm, 150ppm, 200ppm, 250ppm, 300ppm, 350ppm, 400ppm, and each oil sample goes through the following steps: Take a chromatography tube and weigh 1g layer Use silica gel for analysis, suspend in petroleum ether and wet-pack the column. Accurately weigh 5g of the oil sample, dissolve it with 25mL petroleum ether and load the ...

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Abstract

The invention discloses a method for detecting trace lipid-soluble tea polyphenol in vegetable oil, which comprises the following steps of: making a gallic acid ethyl ester standard curve, analyzing effective ingredient content of the lipid-soluble tea polyphenol, making a lipid-soluble tea polyphenol labeled curve, then detecting the absorbance of an oil sample to be detected, and substituting the detected value of the absorbance into the labeled curve to obtain the lipid-soluble tea polyphenol content of the oil sample. The method has the characteristics of simple operation, low cost, good repeatability and high accuracy.

Description

Technical field [0001] The invention belongs to the technical field of detection of vegetable oils, in particular to a method for detecting trace amounts of tea polyphenols in vegetable oils, and more specifically to a method for detecting fat-soluble tea polyphenols in vegetable oils. Background technique [0002] Tea polyphenols (Tea PolypHenols) is a general term for polyphenols in tea. It is one of the main components that form the color and fragrance of tea, and it is also one of the main components of tea with health care functions. Fat-soluble tea polyphenols are the effective extracts of tea tea polyphenols modified by chemical truncation. It is brown oily or powdery, non-toxic and odorless, and is a highly effective bio-antibiotic with specific physiological functions. Oxidant, which can be added to vegetable fats and oils, its antioxidant capacity is comparable to tert-butyl hydroquinone (TBHQ) and greater than propyl gallate (PG), 2,6-di-tert-butyl-p-toluene (BHT), Te...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 崔同王洋王珊珊王晓玲
Owner SHANDONG LUHUA GROUP
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