Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Marburg and Ebola dual-virus fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and dual-virus detection PCR system

A technology of Marburg virus and Ebola virus, applied in the fields of fluorescence/phosphorescence, microbiological determination/inspection, biochemical equipment and methods, etc.

Active Publication Date: 2013-10-30
CHINESE ACAD OF INSPECTION & QUARANTINE
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are ELISA antigen detection, indirect immunofluorescence serological detection, virus isolation, and RT-PCR nucleic acid detection methods for Marburg and Ebola virus detection methods, but the fluorescent quantitative PCR nucleic acid detection method is recognized as a more intuitive method. , a faster detection method suitable for early diagnosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Marburg and Ebola dual-virus fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and dual-virus detection PCR system
  • Marburg and Ebola dual-virus fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and dual-virus detection PCR system
  • Marburg and Ebola dual-virus fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and dual-virus detection PCR system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0041] Marburg of the present invention, the working principle of the new method of fluorescent quantitative PCR detection of Ebola double virus promptly utilizes the qualitative analysis of the change of fluorescent signal, detects in real time the change of the amplification product amount of each cycle in the PCR amplification reaction, through Ct value and The relationship to the standard curve was quantified against the starting template. The present invention also relates to all the contents contained in the above-mentioned detection system.

[0042] The method includes the following steps:

[0043] 1) Design primer probes;

[0044] 2) Select fluorescein according to the instrument;

[0045] 3) Synthetic primers and probes;

[0046] 4) Prepare positive plasmid standard;

[0047] 5) run PCR;

[0048] 6) Data analysis;

[0049] 7) Extract viral RNA for system verification.

[0050] 1. Design of primers and probes

[0051] According to the name of the gene to be stu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Marburg and Ebola dual-virus fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and system, wherein the detection system comprises primers, probes, a Premix EX Taq reaction solution and sterilizing Tris water. As two pairs of primers and probes have very good specificity, the detection system has high sensitivity and is suitable for simultaneously detecting Marburg and Ebola viruses without having cross reaction with other kinds of hemorrhagic fever arbovirus, such as yellow fever, dengue and rift valley fever.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a rapid qualitative and quantitative detection method for dual viruses of Marburg and Ebola, each of which provides a pair of specific primers and probes. Background technique [0002] Marburg virus (MbV), also known as green monkey disease virus and green monkey factor, is the first filovirus discovered by humans, which can cause high mortality in humans and other primates. Hemorrhagic fever, Marburg hemorrhagic fever has a sudden onset, often accompanied by severe headache and discomfort. Muscle pain is a common feature. The incubation period is generally 3 to 9 days, and the longer one can exceed 2 weeks. It can be used as a potential biological disease. The use of terrorist weapons or biological warfare agents is a virulent virus that requires the highest level of biological safety protection (P4 level). The WTO issued an alert saying, "Marburg virus is one of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68G01N21/64
CPCY02A50/30
Inventor 杨宇王静白琳胡孔新徐宝梁
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com