Unlock instant, AI-driven research and patent intelligence for your innovation.

Specific sequence characterized amplified region (SCAR) marker for gametocidal chromosome 2C of aegilops

A technology for chromosomes and gametes, which is applied in the field of gameticidal chromosome 2C-specific SCAR markers of Capt.

Inactive Publication Date: 2013-04-10
HARBIN NORMAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromosome 2S lo , 2S sh , T2B-2S sp.au , 4S lo , 4S sh , 4S sh #2, causing complete sterility in "Chinese Spring" wheat in gametes without these chromosomes and therefore unable to be passed on to the next generation
Chromosome 3C exerts a strong gametocidal effect in 'Chinese Spring' but a mild, semi-lethal effect in other cultivars, suggesting the presence of repressor genes in these cultivars
Many scholars use the banding method to identify offspring, but this method is very cumbersome and requires the combination of related cytological techniques

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Specific sequence characterized amplified region (SCAR) marker for gametocidal chromosome 2C of aegilops
  • Specific sequence characterized amplified region (SCAR) marker for gametocidal chromosome 2C of aegilops
  • Specific sequence characterized amplified region (SCAR) marker for gametocidal chromosome 2C of aegilops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Acquisition of Specific SCAR Marker on Chromosome 2C of Capeatia genus Gametocidal

[0034] 1. Screening and cloning of the gametocidal chromosome 2C-specific RAPD marker OPF03 marker of the genus Goatia

[0035] Using 40 RAPD primers, amplified and screened common wheat "China Spring", common wheat "Chinese Spring"-gametocidal chromosome 2C disomy addition line, and Goatia stigmatis, and isolated and cloned 2C genome-specific amplified fragments .

[0036] Among them, the 20μl system amplified by PCR

[0037] Template DNA 50ng

[0038] 10xPCR buffer 2.5μl

[0039] 1.5mM MgCl 2 2.5μl

[0040] dNTP Mix (2.5mM) 1.5μl

[0041] Random Primer (10μM) 1μl

[0042] r Taq polymerase (5U / μl) 0.25μl

[0043] Distilled sterile water up to 20μl

[0044] 10x PCR buffer was purchased from Treasure Bioengineering (Dalian) Co., Ltd., catalog number R10T1.

[0045] PCR program: pre-denaturation at 94°C for 3 min, denaturation at 94°C for 15 s, anneali...

Embodiment 2

[0068] Example 2, the application of the gametocidal chromosome 2C-specific SCAR marker of the genus Goatia

[0069] with SCAR primers 2C-F 586 , 2C-R 586 F 1 and F 1 F 2 Generation was identified. The common wheat "China Spring" was used as the control.

[0070] The result is as image 3 As shown, 1. is the common wheat "China Spring"; 2-12. is the F obtained by crossing the "China Spring"-Thinopyrum elongatum 7E disomy addition line and "China Spring"-gametocidal chromosome 2C disomy addition line 1 generation; 13. is DL2000Marker; 14~25. is F 1 F 2 generation.

[0071] It can be seen from the figure that in F 1 A product of 586 bp was amplified in all the materials of the first generation, which was in line with theoretical expectations. After sequencing, the fragment had the sequence table sequence 2 from the 5' end of the 405th to 990th nucleotide, which is unique to the gametocidal chromosome 2C, F 1 All generations showed positive results.

[0072] while in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a specific sequence characterized amplified region (SCAR) marker for the gametocidal chromosome 2C of aegilops. The invention provides a kit for detecting the gametocidal chromosome 2C of a plant, a primer in a primer pair has a nucleotide represented by a sequence 3 in a sequence table and the other primer in the primer pair has a nucleotide represented by a sequence 4 inthe sequence table. The gametocidal chromosome 2C is an effective mode for inducing a translocation line, and through the gametocidal chromosome 2C, the excellent properties of many corps can be transferred into the wheat to improve the quality of the wheat. Experiments performed in the invention prove that the development of the SCAR marker has a high value for identifying the gametocidal chromosome 2C transferred into common wheat; and the screening and identification of a large number of hybrid offspring are very complicated, but the development of a gametocidal chromosome 2C molecular marker provides a very convenient and quick approach for tracing and identifying of offspring of the gametocidal chromosome 2C in common wheat and lays a good foundation for analyzing the chromosome configuration of the hybrid offspring.

Description

technical field [0001] The invention relates to a specific SCAR marker for gametocidal chromosome 2C of the genus Goatia, in particular to a kit for detecting gametocidal chromosome 2C under the genetic background of wheat and its application. Background technique [0002] Wheat is one of the important food crops in the world. In China, it is the second largest crop after rice, and wheat production is of great strategic significance to the development of the national economy. Due to the simplification of wheat cultivars, its genetic base is increasingly narrow and its resistance sources are scarce, which greatly limits the further improvement of its yield and quality. There are a large number of genetic variations in close relatives of wheat, which are beneficial gene resources that can be used for wheat improvement. The introduction of these beneficial genes into wheat has always been an enduring research topic for geneticists and breeders. Creating translocation lines is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 郭长虹徐国辉束永俊丛雯雯郭东林
Owner HARBIN NORMAL UNIVERSITY