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Method for culturing Schizophyllum commune

A cultivation method and technology of schizophyllum, applied in the field of plant disease prevention and control, can solve the problems of low yield and achieve the effects of high yield, increased yield and low cost

Inactive Publication Date: 2011-09-07
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under this liquid culture process, every liter of fermentation liquid contains 200-300g mycelium (wet weight), but its output is still low

Method used

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  • Method for culturing Schizophyllum commune

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Effect test

Embodiment 1

[0033] The preparation of embodiment 1 Schizophyllum hyphae

[0034] 1. Strain activation: transfer the parent species of Schizophyllum to PDA solid medium (20% potato starch, 20% glucose, 20% agar, and the rest are water), cultivate it at 24°C for 10 days, and the hyphae are overgrown flat.

[0035] 2. Fermentation culture of Schizophyllum

[0036] (1) Preparation of liquid fermentation medium: Weigh the following components respectively, 60g soluble starch, 55g glucose, 30g wheat flour, 40g corn flour, 6g yeast extract, 10g soybean flour, 6g peptone, 1gKH 2 PO 4 , 0.5gMgSO 4 , 10mg VB 1 , add these substances to water and make the volume to 1000ml, and mix well.

[0037] (2) Preparation of first-level strains: the plate mycelium obtained in step 1 is cut into fritters less than 1 mm, and the Schizophyllum mycelium is transferred to a 100 ml fermentation medium according to the inoculum size of 0.4 g mycelia / 100 ml. In 500ml shake flask, at 24 ℃, 200rpm shake flask cult...

Embodiment 2

[0040] The preparation of embodiment 2 Schizophyllum hyphae

[0041] 1. Strain activation: transfer the parent species of Schizophyllum to PDA solid medium (20% potato starch, 20% glucose, 20% agar, and the rest are water), and cultivate it at 26°C for 10 days, and the mycelia are overgrown flat.

[0042] 2. Fermentation culture of Schizophyllum

[0043] (1) Preparation of fermentation medium: Weigh the following components respectively, 40g soluble starch, 40g glucose, 20g wheat flour, 20g corn flour, 3g yeast extract, 5g soybean flour, 2g peptone, 0.1g KH 2 PO 4 , 0.5gMgSO 4 , 10mg VB 1 , Add these substances to 1000ml water and mix well.

[0044] (2) First-level strain preparation: the plate mycelium that step 1 obtains is cut into the fritter that is less than 1mm, according to the inoculum amount of 0.6g mycelia / 100ml, Schizophyllum mycelium is transferred to the fermentation medium that 100ml is housed In a 500ml shake flask, the shake flask was cultured at 26°C an...

Embodiment 3

[0047] The preparation of embodiment 3 Schizophyllum hyphae

[0048] 1. Strain activation: transfer the parent species of Schizophyllum to PDA solid medium (20% potato starch, 20% glucose, 20% agar, and the rest are water), cultivate it at 28°C for 10 days, and the hyphae are overgrown flat.

[0049] 2. Fermentation culture of Schizophyllum

[0050] (1) Preparation of fermentation medium: Weigh the following components respectively, 60g soluble starch, 55g glucose, 30g wheat flour, 40g corn flour, 6g yeast extract, 10g soybean flour, 6g peptone, 1gKH 2 PO 4 , 0.5gMgSO 4 , 10mg VB 1 , Add these substances to water to make up to 1000ml and mix well.

[0051] (2) Preparation of first-level strains: the plate mycelium obtained in step 1 is cut into fritters less than 1mm, and the Schizophyllum mycelium is transferred to a 100ml fermentation medium according to the inoculum size of 0.8g mycelium / 100ml In a 500ml shake flask, cultivate it at 28°C and 260rpm for 6 days to obtai...

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Abstract

The invention discloses a method for culturing Schizophyllum commune. The method comprises the steps of activating a Schizophyllum commune mother strain to obtain a first-level strain; and then fermenting and culturing the first-level strain in a liquid fermentation medium for 4-6 days at temperature of 24-28 DEG C and rotation speed of 200-260 rpm / min to obtain a fermentation mixed solution; filtering the fermentation mixed solution; collecting Schizophyllum commune hyphae; drying and crushing the Schizophyllum commune hyphae and the like. The Schizophyllum commune with high yield is fermented and cultured by using the method disclosed by the invention; mycelia with wet weight of 400-480 g or dry weight of 54-60 g can be obtained from each liter of fermentation strain solution; and compared with the prior art, the Schizophyllum commune has largely increased yield.

Description

[0001] This application is a divisional application of the patent application with the application number: 200810247561.6, the application date is December 30, 2008, and the invention name is "Schizophyllum protein extract and its preparation method and application". technical field [0002] The invention belongs to the field of plant disease prevention and control, and specifically relates to a schizophyllum protein extract and its preparation method and application; in addition, it also relates to a schizophyllum culture method and culture medium. Background technique [0003] Plant virus disease is similar to human cancer, and there is no very effective prevention and treatment method at present. Tobacco mosaic virus (TMV) is one of the most economically important viruses, which can infect more than 300 species of plants such as Solanaceae, Cucurbitaceae, and Cruciferae, and cause serious damage. Rely on breeding and chemical agents to solve the problem, but the effect is...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 周莹李兴红严红燕继晔卢娜
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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