Method for culturing Schizophyllum commune

A cultivation method and technology of schizophyllum, applied in the field of plant disease prevention and control, can solve the problems of low yield and achieve the effects of high yield, increased yield and low cost

Inactive Publication Date: 2011-09-07
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Under this liquid culture process, every liter of fermentation liquid

Method used

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  • Method for culturing Schizophyllum commune

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Example Embodiment

[0033] Example 1 Preparation of Schizophyllum hyphae

[0034] 1. Strain activation: transfer the mother species of Schizophyllum to PDA solid medium (potato starch 20%, glucose 20%, agar 20%, the rest is water), cultured at 24°C for 10 days, the hyphae are overgrown flat.

[0035] 2. Fermentation culture of Schizophyllum

[0036] (1) Preparation of liquid fermentation medium: Weigh the following components respectively, 60g soluble starch, 55g glucose, 30g wheat flour, 40g corn flour, 6g yeast extract, 10g soybean flour, 6g peptone, 1gKH 2 PO 4 , 0.5gMgSO 4 , 10mg VB 1 , Add these substances into water and dilute to 1000ml, and mix well.

[0037] (2) Preparation of first-level strains: cut the plate hyphae obtained in step 1 into small pieces less than 1mm, and transfer the Schizophyllum hyphae into a 100ml fermentation medium at an inoculum of 0.4g hyphae / 100ml In a 500ml shake flask, culture in a shake flask at 24° C. and 200 rpm for 6 days to obtain a first-level strain, with a co...

Example Embodiment

[0040] Example 2 Preparation of Schizophyllum hyphae

[0041] 1. Strain activation: transfer the mother species of Schizophyllum to PDA solid medium (potato starch 20%, glucose 20%, agar 20%, the rest is water), cultured at 26℃ for 10 days, the hyphae are overgrown flat.

[0042] 2. Fermentation culture of Schizophyllum

[0043] (1) Preparation of fermentation medium: Weigh the following ingredients respectively, 40g soluble starch, 40g glucose, 20g wheat flour, 20g corn flour, 3g yeast extract, 5g soybean flour, 2g peptone, 0.1gKH 2 PO 4 , 0.5gMgSO 4 , 10mg VB 1 , Just add these substances into 1000ml water and mix well.

[0044] (2) Preparation of primary strain: cut the flat hyphae obtained in step 1 into small pieces less than 1mm, and transfer the Schizophyllum hyphae into a 100ml fermentation medium at an inoculum of 0.6g hyphae / 100ml In a 500ml shake flask, culture in a shake flask at 26°C and 240rpm for 6 days to obtain a first-class strain (concentration of 4.2g / 100ml).

[00...

Example Embodiment

[0047] Example 3 Preparation of Schizophyllum hyphae

[0048] 1. Strain activation: transfer the mother species of Schizophyllum to PDA solid medium (potato starch 20%, glucose 20%, agar 20%, the rest is water), cultured at 28°C for 10 days, the hyphae are overgrown flat.

[0049] 2. Fermentation culture of Schizophyllum

[0050] (1) Preparation of fermentation medium: Weigh the following components respectively, 60g soluble starch, 55g glucose, 30g wheat flour, 40g corn flour, 6g yeast extract, 10g soybean flour, 6g peptone, 1gKH 2 PO 4 , 0.5gMgSO 4 , 10mg VB 1 , Add these substances to water and dilute to 1000ml and mix well.

[0051] (2) Preparation of primary strain: cut the flat hyphae obtained in step 1 into small pieces less than 1mm, and transfer the Schizophyllum hyphae into a 100ml fermentation medium at an inoculum of 0.8g hyphae / 100ml In a 500ml shake flask, cultured in a shake flask at 28°C and 260rpm for 6 days to obtain a first-level strain (concentration 4.1g / 100ml). ...

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Abstract

The invention discloses a method for culturing Schizophyllum commune. The method comprises the steps of activating a Schizophyllum commune mother strain to obtain a first-level strain; and then fermenting and culturing the first-level strain in a liquid fermentation medium for 4-6 days at temperature of 24-28 DEG C and rotation speed of 200-260 rpm/min to obtain a fermentation mixed solution; filtering the fermentation mixed solution; collecting Schizophyllum commune hyphae; drying and crushing the Schizophyllum commune hyphae and the like. The Schizophyllum commune with high yield is fermented and cultured by using the method disclosed by the invention; mycelia with wet weight of 400-480 g or dry weight of 54-60 g can be obtained from each liter of fermentation strain solution; and compared with the prior art, the Schizophyllum commune has largely increased yield.

Description

[0001] This application is a divisional application of the patent application with the application number: 200810247561.6, the application date is December 30, 2008, and the invention name is "Schizophyllum protein extract and its preparation method and application". technical field [0002] The invention belongs to the field of plant disease prevention and control, and specifically relates to a schizophyllum protein extract and its preparation method and application; in addition, it also relates to a schizophyllum culture method and culture medium. Background technique [0003] Plant virus disease is similar to human cancer, and there is no very effective prevention and treatment method at present. Tobacco mosaic virus (TMV) is one of the most economically important viruses, which can infect more than 300 species of plants such as Solanaceae, Cucurbitaceae, and Cruciferae, and cause serious damage. Rely on breeding and chemical agents to solve the problem, but the effect is...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 周莹李兴红严红燕继晔卢娜
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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