Hard endosperm high-quality protein corn selfing line and breeding method of hybrid thereof

A high-quality protein, inbred line technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of low yield and poor disease resistance, and achieve high yield, strong resistance, and poor disease resistance. Effect

Inactive Publication Date: 2011-09-07
FOOD CROPS RES INST YUNNAN ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects and deficiencies of low yield and poor disease resistance caused by the floury endo

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0032] (1) Using the subtropical high-quality protein maize population Pool31QPM, self-breeding was carried out for 8 consecutive generations in Kaiyuan City, Yunnan Province in spring, Kunming City in summer, and Jinghong City, Yunnan Province in winter by ear row method , the endosperm modification phenotype was identified on a light table for each selfing generation, that is, the grains were placed on a general medical film viewing lamp to observe the degree of transparency of the grains, and the grains with an endosperm modification degree of 2 to 3 were selected (2=75% transparent, 3 =50% transparent); At the same time, the grains screened for each generation are planted in the field, and under the conditions of natural disease in the field, select resistance to maize leaf spot disease, that is, corn leaf spot disease level ≤ 3, and resistance to corn small spot disease, that is, corn small spot disease Disease level ≤ 3 and resistance to corn ear rot, i.e. ear rot disease...

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Abstract

The invention discloses a hard endosperm high-quality protein corn selfing line and a breeding method of a hybrid thereof. The breeding of the selfing line comprises the following steps: performing 5-8 generations of selfing breeding in different places and different seasons with a head progeny row method by use of the high-quality protein corn germ plasm; identifying the endosperm modification phenotype with a light table for each generation of selfing; selecting the kernels with 2-3 level of endosperm modification; planting the screened kernels of each generation in the field, and screening to resist the local major diseases; and performing selfing breeding of the individual strains with a proper plant height in the childbearing period to obtain the hard endosperm high-quality protein corn selfing line. The breeding of the hybrid comprises the following steps: comparing the hybrid combination obtained by the hybridization of the selfing line; performing regional and production test identification; selecting a new hybrid combination to perform quality analysis and identifying the endosperm modification phenotype with a light table; and selecting the kernel of which the lysine content is greater than or equal to 0.40% of and the endosperm modification degree is less than or equal to 2 as the hard endosperm high-quality protein corn hybrid. The bred selfing line or hybrid has strong adaptability, high yield and strong disease resistance, while the endosperm hardness can reach the level 1-2.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, and in particular relates to a method for breeding hard endosperm high-quality protein corn inbred lines and hybrids thereof. Background technique [0002] High-quality protein maize (Quality Protein Maize, abbreviated as QPM) refers to the Opaque-2 The new high-quality corn and its modified gene contain twice the lysine content of ordinary corn, which has higher application value and economic benefits than ordinary corn. It is an important high-efficiency livestock and poultry feed, and the high content of lysine Acid plays an important role in the growth and development of the human body. Feed tests at home and abroad show that raising pigs with high-quality protein corn as the main feed can increase daily weight gain by more than 30% compared with ordinary corn feed, and feeding chickens can increase egg production by more than 15%, and the benefits are very significant. [0003] Since...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 番兴明谭静陈洪梅
Owner FOOD CROPS RES INST YUNNAN ACADEMY OF AGRI SCI
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