Method for preparing silk fibroin/copolymer hydrogel
A technology of silk fibroin and hydrogel, applied in the field of hydrogel, can solve the problems of poor mechanical properties of hydrogel and no improvement of biocompatibility, etc., and achieve the effect of good mechanical properties
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Embodiment 1
[0025] (1) Natural mulberry silk was degummed by boiling 0.05% sodium carbonate solution for 30 minutes, and the pure mulberry silk fibroin protein fiber was obtained after repeating 3 times. The silk fibroin was dissolved in LiBr:C 2 h 5 OH=40:60 (mass ratio) Stir and dissolve in a water bath at a constant temperature of 80±2°C to obtain an aqueous solution of regenerated silk fibroin. After dialysis treatment, a silk fibroin solution with a mass fraction of about 5% was formed.
[0026] (2) Using stannous octoate as a catalyst, lactide and polyethylene glycol (1000) in a ratio of 1:1, the PLA-PEG-PLA copolymer was synthesized by ring-opening reaction in an oil bath at 130°C for 24 hours, and the The resulting copolymer was subjected to NMR analysis to obtain figure 1 ,Depend on figure 1 It can be seen that the PLA-PEG-PLA copolymer was successfully prepared.
[0027] (3) Place the PLA-PEG-PLA copolymer solution and the silk fibroin solution prepared in steps (1) and (2)...
Embodiment 2
[0029] (1) Natural mulberry silk was degummed by boiling 0.05% sodium carbonate solution for 30 minutes, and the pure mulberry silk fibroin protein fiber was obtained after repeating 3 times. The silk fibroin was dissolved in LiBr:C 2 h 5 OH=40:60 (mass ratio) Stir and dissolve in a water bath at a constant temperature of 80±2°C to obtain an aqueous solution of regenerated silk fibroin. After dialysis treatment, a silk fibroin solution with a mass fraction of about 5% was formed.
[0030] (2) Using stannous octoate as a catalyst, lactide and polyethylene glycol (1000) at a ratio of 1:1, the PLA-PEG-PLA copolymer was synthesized by ring-opening reaction in an oil bath at 130°C for 24 hours.
[0031] (3) Add the PLA-PEG-PLA copolymer prepared in steps (1) and (2) to the silk fibroin solution and let it stand at about 37°C. When the mass ratio of the added PLA-PEG-PLA copolymer (molecular weight 1000) to silk fibroin is 5:1, the gelation time required to prepare the hydrogel i...
Embodiment 3
[0034] (1) Natural mulberry silk was degummed by boiling 0.05% sodium carbonate solution for 30 minutes, and the pure mulberry silk fibroin protein fiber was obtained after repeating 3 times. The silk fibroin was dissolved in LiBr:C 2 h 5 OH=40:60 (mass ratio) Stir and dissolve in a water bath at a constant temperature of 80±2°C to obtain an aqueous solution of regenerated silk fibroin. After dialysis treatment, a silk fibroin solution with a mass fraction of about 5% was formed.
[0035] (2) Using stannous octoate as a catalyst, lactide and polyethylene glycol (1000) at a ratio of 1:1, the PLA-PEG-PLA copolymer was synthesized by ring-opening reaction in an oil bath at 130°C for 24 hours.
[0036] (3) Add the PLA-PEG-PLA copolymer prepared in steps (1) and (2) to the silk fibroin solution and let it stand at about 37°C. When the mass ratio of the added PLA-PEG-PLA copolymer (molecular weight 1000) to silk fibroin is 3:1, the gel time required to prepare the hydrogel is about...
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