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Method for preparing silk fibroin/copolymer hydrogel

A technology of silk fibroin and hydrogel, applied in the field of hydrogel, can solve the problems of poor mechanical properties of hydrogel and no improvement of biocompatibility, etc., and achieve the effect of good mechanical properties

Inactive Publication Date: 2011-09-07
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its synthetic raw materials, polylactic acid and polyethylene glycol, have good biocompatibility, but due to their temperature-sensitive and reversible physical cross-linking properties, the mechanical properties of the formed hydrogel are poor.
Christine Hiemstra, Zhiyuan Zhong et al improved the shortcomings of PLA-PEG-PLA copolymer hydrogel mechanical properties by synthesizing PEG-PLA star copolymers (see: Christine Hiemstra, Zhiyuan Zhong et al. Biomacromolecules 7(2006) 2790-2795), but the biocompatibility of PLA-PEG-PLA copolymers has not been improved

Method used

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  • Method for preparing silk fibroin/copolymer hydrogel
  • Method for preparing silk fibroin/copolymer hydrogel
  • Method for preparing silk fibroin/copolymer hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Natural mulberry silk was degummed by boiling 0.05% sodium carbonate solution for 30 minutes, and the pure mulberry silk fibroin protein fiber was obtained after repeating 3 times. The silk fibroin was dissolved in LiBr:C 2 h 5 OH=40:60 (mass ratio) Stir and dissolve in a water bath at a constant temperature of 80±2°C to obtain an aqueous solution of regenerated silk fibroin. After dialysis treatment, a silk fibroin solution with a mass fraction of about 5% was formed.

[0026] (2) Using stannous octoate as a catalyst, lactide and polyethylene glycol (1000) in a ratio of 1:1, the PLA-PEG-PLA copolymer was synthesized by ring-opening reaction in an oil bath at 130°C for 24 hours, and the The resulting copolymer was subjected to NMR analysis to obtain figure 1 ,Depend on figure 1 It can be seen that the PLA-PEG-PLA copolymer was successfully prepared.

[0027] (3) Place the PLA-PEG-PLA copolymer solution and the silk fibroin solution prepared in steps (1) and (2)...

Embodiment 2

[0029] (1) Natural mulberry silk was degummed by boiling 0.05% sodium carbonate solution for 30 minutes, and the pure mulberry silk fibroin protein fiber was obtained after repeating 3 times. The silk fibroin was dissolved in LiBr:C 2 h 5 OH=40:60 (mass ratio) Stir and dissolve in a water bath at a constant temperature of 80±2°C to obtain an aqueous solution of regenerated silk fibroin. After dialysis treatment, a silk fibroin solution with a mass fraction of about 5% was formed.

[0030] (2) Using stannous octoate as a catalyst, lactide and polyethylene glycol (1000) at a ratio of 1:1, the PLA-PEG-PLA copolymer was synthesized by ring-opening reaction in an oil bath at 130°C for 24 hours.

[0031] (3) Add the PLA-PEG-PLA copolymer prepared in steps (1) and (2) to the silk fibroin solution and let it stand at about 37°C. When the mass ratio of the added PLA-PEG-PLA copolymer (molecular weight 1000) to silk fibroin is 5:1, the gelation time required to prepare the hydrogel i...

Embodiment 3

[0034] (1) Natural mulberry silk was degummed by boiling 0.05% sodium carbonate solution for 30 minutes, and the pure mulberry silk fibroin protein fiber was obtained after repeating 3 times. The silk fibroin was dissolved in LiBr:C 2 h 5 OH=40:60 (mass ratio) Stir and dissolve in a water bath at a constant temperature of 80±2°C to obtain an aqueous solution of regenerated silk fibroin. After dialysis treatment, a silk fibroin solution with a mass fraction of about 5% was formed.

[0035] (2) Using stannous octoate as a catalyst, lactide and polyethylene glycol (1000) at a ratio of 1:1, the PLA-PEG-PLA copolymer was synthesized by ring-opening reaction in an oil bath at 130°C for 24 hours.

[0036] (3) Add the PLA-PEG-PLA copolymer prepared in steps (1) and (2) to the silk fibroin solution and let it stand at about 37°C. When the mass ratio of the added PLA-PEG-PLA copolymer (molecular weight 1000) to silk fibroin is 3:1, the gel time required to prepare the hydrogel is about...

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Abstract

The invention discloses a method for preparing a silk fibroin / polylactic acid-polyethylene glycol-polylactic acid (PLA-PEG-PLA) copolymer hydrogel, which comprises the following steps of: (1) preparing silk fibroin aqueous solution and PLA-PEG-PLA copolymer; and (2) adding the copolymer into the silk fibroin aqueous solution, and standing at the temperature of between 35 and 39 DEG C to obtain the hydrogel. The hydrogel prepared by the method has high mechanical property and biocompatibility; in addition, the setting time of the hydrogel can be adjusted by adjusting the concentration of the added PLA-PEG-PLA copolymer, the ratio of PEG chain segments, and molecular weight, and the mechanical property of the hydrogel can be adjusted by adjusting the concentration of the added silk fibroin aqueous solution.

Description

technical field [0001] The invention belongs to the field of hydrogel materials, in particular to a hydrogel composed of silk fibroin and copolymer PLA-PEG-PLA. Background technique [0002] Among many biomedical polymer materials, polymer hydrogel is one of the research hotspots today. Polymer hydrogel is a polymer that can swell in water and retain a large amount of water without dissolving. Because the hydrogel polymer network is filled with a large amount of water, the whole material has a fluid property, which is similar to the body tissue filled with a large amount of aqueous solution, and the soft and wet surface greatly reduces the impact of the material on the surrounding tissue. Stimulation makes the hydrogel have good biocompatibility and has great development potential in the direction of drug release and human tissue engineering. [0003] In the prior art, the hydrogel materials that belong to research hotspots and have made some progress include collagen, chi...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L89/00C08L67/04C08G63/664
Inventor 钟天翼左保齐
Owner SUZHOU UNIV
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