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Micro-slit-structure-based full PDMS (polydimethylsiloxane) micro-fluidic cell capturing chip and manufacturing method thereof

A microfluidic, cell technology, applied in biochemical equipment and methods, enzymology/microbiology devices, bioreactors/fermenters for specific purposes, etc. Achieve the effects of high capture efficiency, simple and feasible modification and integration operations, and simple production methods

Inactive Publication Date: 2011-09-07
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantage is that it requires high micro-processing technology, which is difficult to achieve in ordinary chemical laboratories.

Method used

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  • Micro-slit-structure-based full PDMS (polydimethylsiloxane) micro-fluidic cell capturing chip and manufacturing method thereof
  • Micro-slit-structure-based full PDMS (polydimethylsiloxane) micro-fluidic cell capturing chip and manufacturing method thereof
  • Micro-slit-structure-based full PDMS (polydimethylsiloxane) micro-fluidic cell capturing chip and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Fabrication of a double-layer structure cell capture chip:

[0028] 1. Use as figure 1 Prepare PDMS microchannel chips in the manner shown.

[0029] Step 1. Fabrication of glass channels: glass chip channels are prepared using standard photolithography and chemical wet etching techniques. Using a commercial uniform colloidal chromium plate, photolithography is carried out according to the channel design pattern, and the etching depth is 30-50 μm;

[0030] Step 2. Make the upper and lower layers of polydimethylsiloxane (PDMS) microchannel sheets: use the above-mentioned glass chip as a negative template, cast a certain amount of PDMS on it, heat and solidify, and get a corresponding positive mold after peeling off structured PDMS chips. After the PDMS positive mold is treated with air plasma, the surface is quickly spin-coated with an aqueous solution of polyvinyl alcohol (PVA) with a mass percentage concentration of 0.1-0.5%, and dried under an infrared lamp...

Embodiment 2

[0034] Example 2. Using a double-layer structure cell capture chip to capture and release cells:

[0035] In order to characterize the capture effect of the chip on cells, we used different channel combinations to make micro-slits and captured the suspended BGC-823 cell samples. The results are as follows: image 3 shown. The steps are:

[0036] Step 1. Inject the cell suspension at the entrance of the upper channel. Due to the difference in hydrostatic pressure, the cells flow to the solution to the outlet and shunt to the lower channel. Blocked by the slit, the cells are continuously arranged and aggregated on the slit along the flow direction to form a capture. Excess cells are discharged from the outlet along with the suspension; the captured cells can also be washed while maintaining the pressure.

[0037] Step 2. Aspirate the sample solution from the inlet, inject the buffer solution into the inlet and outlet of the lower channel, reverse the pressure of the liquid, ev...

Embodiment 3

[0038] Example 3. Using a double-layer structure cell capture chip to capture several cells or a single cell:

[0039] In order to characterize the capture performance of the chip for a small number of cells, we captured several and single cells by reducing the length of the slit and using staggered bonding. The results are as follows Figure 4 shown.

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Abstract

The invention discloses a double-layer structure cell capturing chip, which is a micro-slit-structure-based full PDMS (polydimethylsiloxane) micro-fluidic cell capturing chip. The cell capturing chip consists of an upper solidified polydimethylsiloxane thin sheet and a lower solidified polydimethylsiloxane thin sheet, wherein micro-channels with different sizes and shapes are formed in the polydimethylsiloxane thin sheets respectively; the upper layer channel and the lower layer channel are staggered in parallel along edges; and 10+ / -5 mu m slits are formed by the channels in an aligning and bonding method, and the length and the quantity of the slits can be adjusted and controlled according to different channel combination and alignment methods so that the amount of captured cells can becontrolled. Based on a hydrodynamics principle, the chip blocks cells by using a slit structure so as to capture a limited quantity of cells, and simultaneously, the cell release can be controlled byadjusting the fluid pressure to realize repeated cell capturing. The chip is made by a conventional chip manufacturing method and can capture and release a single cell and a plurality of cells; moreover, the chip can be used for integrating a microelectrode for monitoring captured cells in real time. The invention also discloses a manufacturing method and process of the chip.

Description

technical field [0001] The invention relates to a PDMS microfluidic cell capture chip and a microfluidic cell capture control. Background technique [0002] Cells are very small basic units with unique life activities. Traditional cell research usually takes a large number of cell samples as the object, hoping to obtain the general properties of the same kind of cells. However, even cells of the same type are more or less variable, so this kind of analysis can only provide the average response of the whole cell sample, and ignore the detailed information of single cells and cells. Heterogeneity in cellular behavior is inherent in both bacterial and animal eukaryotic cells and is ubiquitous in all complex biological events such as cell growth, differentiation, and infection. Therefore, in biological and chemical research, the controllability of experimental conditions, especially the control of single cells, will help scientists understand basic life processes such as cell r...

Claims

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Application Information

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IPC IPC(8): C12M1/00
Inventor 徐静娟勾洪磊陈洪渊
Owner NANJING UNIV
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