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Method for producing enramycin by fermentation

A technology of enramycin and fermentation liquid, which is applied in the field of fermentation, can solve problems such as insufficient dissolved oxygen, and achieve the effect of improving the fermentation level

Active Publication Date: 2011-09-07
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method for fermentative production of enramycin, in order to solve the problem of insufficient dissolved oxygen in the fermentation liquid of existing fermentative production of enramycin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) Incline cultivation:

[0016] Streptomyces fungicidious CGMCC 4.1312 T (purchased from the Institute of Microbiology, Chinese Academy of Sciences) were inoculated on a solid slant medium under sterile conditions, and incubated at a constant temperature of 28°C for 8 days;

[0017] Wherein, the solid slant medium includes the following components: corn flour 20g / L, peptone 1g / L, ZnSO 4 .7H 2 O 0.1g / L, MnCl 2 .4H 2 O 0.02g / L, agar 15g / L.

[0018] 2) Fermentation culture:

[0019] Inoculate the bacteria cultured in step 1) on the fermentation medium in the fermentation bottle under aseptic conditions, ferment for 40 hours at 28° C., 300 r / min, and replenish water in the fermentation broth. The amount of water replenishment is 5% of the volume of the fermentation broth , the number of fermentation days is 8 days, and the average fermentation level is 850U / ml;

[0020] Wherein, the fermentation medium includes the following components: 100 g / L of corn flour, 10 g / L...

Embodiment 2

[0022] 1) Incline cultivation:

[0023] Streptomyces fungicidious CGMCC 4.1312 T ) was inoculated on a solid slant medium under sterile conditions, and incubated at a constant temperature of 28°C for 8 days;

[0024] Wherein, the solid slant medium includes the following components: corn flour 20g / L, peptone 1g / L, ZnSO 4 .7H 2 O 0.1g / L, MnCl 2 .4H 2 O 0.02g / L, agar 15g / L.

[0025] 2) Fermentation culture:

[0026] Inoculate the bacteria cultured in step 1) on the fermentation medium in the fermentation bottle under aseptic conditions, ferment for 72 hours at 28° C., 300 r / min, and replenish water in the fermentation broth. The amount of water replenishment is 10% of the volume of the fermentation broth , the number of fermentation days is 8 days, and the average fermentation level is 1210U / ml;

[0027] Wherein, the fermentation medium includes the following components: 100 g / L of corn flour, 10 g / L of corn steep liquor, 3 g / L of ammonium sulfate, 0.1 g / L of zinc sulfate...

Embodiment 3

[0029] 1) Incline cultivation:

[0030] Streptomyces fungicidious CGMCC 4.1312 T ) was inoculated on a solid slant medium under sterile conditions, and incubated at a constant temperature of 28°C for 8 days;

[0031] Wherein, the solid slant medium includes the following components: corn flour 20g / L, peptone 1g / L, ZnSO 4 .7H 2 O 0.1g / L, MnCl 2 .4H 2 O 0.02g / L, agar 15g / L.

[0032] 2) Fermentation culture:

[0033] Inoculate the bacteria cultured in step 1) on the fermentation medium in the fermentation bottle under aseptic conditions, ferment for 96 hours at 28° C., 300 r / min, and replenish water in the fermentation broth. The amount of water replenishment is 20% of the volume of the fermentation broth , the number of fermentation days is 8 days, and the average fermentation level is 960U / ml;

[0034] Wherein, the fermentation medium includes the following components: 100 g / L of corn flour, 10 g / L of corn steep liquor, 3 g / L of ammonium sulfate, 0.1 g / L of zinc sulfate,...

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Abstract

The invention discloses a method for producing enramycin by fermentation. Cabicidin streptomyces avermitilis is used as a fermentation strain in the method; and water is supplied to the fermentation solution in the fermentation process of constant temperature culture in a fermentation medium, so that the dissolved oxygen quantity of the fermentation solution is improved, and the fermentation level of the enramycin is improved. The method is simple and feasible; and after a proper amount of water is supplied at proper time in the fermentation culture process, the fermentation level of the enramycin can be improved by about 130 percent.

Description

technical field [0001] The invention relates to the technical field of fermentation, in particular to a method for fermenting and producing enramycin. Background technique [0002] Enramycin (Enramycin), also known as Enlaimycin, Ensu, Anlemycin, Persistomycin, is a cyclic polypeptide antibiotic composed of 13 different types of 17 amino acid molecules and fatty acid molecules. Amino acids are composed of cyclic polypeptides, and fatty acid molecules are connected to the terminal aspartic acid. According to the difference of the terminal fatty acid molecules, it is divided into enramycin A and enramycin B. Enramycin is a mixture of these two components , a polypeptide antibiotic produced by fermentation of actinomyces (Streptomyces fungieidious) isolated from soil. Enramycin has a strong inhibitory effect on Gram-positive bacteria, mainly hindering the synthesis of bacterial cell walls. The sensitive bacteria include various Gram-positive cocci such as Staphylococcus aureu...

Claims

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Application Information

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IPC IPC(8): C12P21/04C12R1/465
Inventor 李荣杰崔刚尚海涛付松
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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