Method for increasing content of nutrient components in rice by thickening aleurone layer

A technology for nutrient composition and aleurone layer, applied in the field of genetic engineering, can solve problems such as restricting the popularization and application of cultivated varieties, and achieve the effect of increasing grain width and thousand-grain weight

Inactive Publication Date: 2011-09-14
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these exogenous gene introduction methods can only change the content of s...

Method used

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  • Method for increasing content of nutrient components in rice by thickening aleurone layer
  • Method for increasing content of nutrient components in rice by thickening aleurone layer
  • Method for increasing content of nutrient components in rice by thickening aleurone layer

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1, utilize the thickening of aleurone layer to select and breed the paddy rice that nutrient component content improves

[0024] 8,000 Zhonghua 11 rice seeds (wild type, starting rice) were mutagenized with 60 mM ethyl methane sulfonate (EMS), and the seeds were planted to obtain 1,327 fertile M1 Plants (mutation first generation); a total of 8,927 single ears were harvested from M1 plants. Then 10 seeds were taken out from each single panicle, and preliminary screening was carried out by half-seed screening.

[0025] Wherein, the mutagenesis method is as follows:

[0026] Wash the seeds with water to remove dust, divide them into 200 grains each, put them into a 500ml blue cap bottle, add 100ml of water to soak, and shake at 125rpm overnight.

[0027] Pour off the water, add 60 mM EMS solution (prepared with 66 mM phosphate buffer, pH 7.0) for 24 hours of mutagenesis, and also shake at 125 rpm.

[0028] Remove the EMS solution, rinse with 100 ml of water ...

Embodiment 2

[0033] Embodiment 2, to ta-1 and ta-2 strain correlation yield character and nutrient composition determination

[0034] The other embryonated seeds of mutants ta-1 and ta-2 were planted. Use mercuric chloride to sterilize half of the seeds with embryos, sow them on 1 / 2MS medium, culture at 25°C, light 1,500-2,000lx, 16h / d, and wait until the seedlings grow 2-3 complete leaves. After 2-3 days of seedling hardening, move the seedlings to the soil, 30°C day / 20°C night, light 5,000-10,000lx, 12h / d and cultivate until firm. Harvest seeds (M2 generation) for further analysis and identification. The M2 generation is propagated to obtain the M3 generation and the M4 generation.

[0035] The yield shape and nutritional components of the ta-1 and ta-2 lines were determined, and the flowering period of the lines was two weeks later than that of the wild type. Investigating the grain length, grain thickness, grain width, and thousand-grain weight of the seeds, it was found that the se...

Embodiment 3

[0043] Embodiment 3, to the stability determination of ta-1 and ta-2 strain

[0044] Plant and reproduce the ta-1 and ta-2 strains screened out in Example 2, detect the content of each nutrient component according to the above-mentioned national standards respectively, evaluate and verify the stability of rice materials, the results show that ta-1 and ta-2 are in the greenhouse (planting M3 generation) and field planting (planting M4 generation) of two consecutive generations of materials have obvious high nutritional quality, and high nutritional and high-quality rice varieties have been obtained. Wherein, the mass percent content of the M3 generation protein of the ta-1 strain is 12.1%, the zinc content is 29.15%, the iron content is 16.55%, and the mass percentage composition of the fat is 4.45%; The mass percentage of M4 generation protein is 12%, the zinc content is 29.6%, the iron content is 44%, the mass percentage of fat is 3.7%; the mass percentage of M3 generation pr...

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Abstract

The invention discloses a method for increasing the content of nutrient components in rice by thickening an aleurone layer. The method for cultivating the rice with increased content of the nutrient components comprises a step of thickening the aleurone layer of initial rice to obtain target rice with higher content of nutrient components than the initial rice. In the invention, the high nutriment rice is bred by thickening the aleurone layer of the rice; the problem that only the content of special nutrient components can be changed when special exogenous genes are inducted at present is solved; and compared with wild-type rice, the length, the width and the thousand seed weight of seeds of the rice cultivated by using the method are all increased and the content of nutrient components such as proteins and materials of zinc, iron and the like in the seeds is obviously increased.

Description

technical field [0001] The invention relates to a genetic engineering method for cultivating high-nutrition and high-quality crops by increasing the thickness of the aleurone layer, in particular to a method for increasing the nutritional content of rice by thickening the aleurone layer. Background technique [0002] The endosperm of cereal crops, especially rice, wheat and corn, provides the largest food source for human beings. The triploid endosperm is the product of double fertilization. Taking rice as an example, its mature endosperm consists of two parts: the outer aleurone layer composed of 2-3 layers of cells and the inner starch endosperm that loses its cell structure. The aleurone endosperm, composed of living cells, acts like a pocket around the lifeless starchy endosperm that undergoes a programmed death process. The main component of starch endosperm is macromolecular carbohydrates composed of long-chain polysaccharides, which mainly provide energy for human b...

Claims

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Application Information

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IPC IPC(8): A01C1/00
Inventor 刘春明吴小坝龚化勤宋秀芬
Owner INST OF BOTANY CHINESE ACAD OF SCI
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